中医药信息
中醫藥信息
중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2015年
3期
66-70
,共5页
曲池%神门%足三里%Bcl-2%Bax%电针%心肌肥厚
麯池%神門%足三裏%Bcl-2%Bax%電針%心肌肥厚
곡지%신문%족삼리%Bcl-2%Bax%전침%심기비후
Quchi%Shenmen%Zusanli%Bcl-2%Bax%Electroacupuncture%Cardiac hypertrophy
目的：探讨电针曲池穴、神门穴、足三里穴对心肌肥厚模型大鼠Bcl－2及Bax影响。方法：50只Wistar大鼠随机分为模型组、假手术组、曲池＋足三里组、神门＋足三里组、曲池＋神门＋足三里组，每组10只，胸主动脉缩窄法建立大鼠心肌肥厚模型，电针组连续治疗14天，通过实时定量PCR及West-ern blot测定心肌组织中Bcl－2、Bax mRNA及蛋白的表达水平。结果：曲池＋足三里组、神门＋足三里组、曲池＋神门＋足三里组Bcl－2及Bax mRNA及蛋白的表达水平在治疗后与模型组相比，有显著性差异（P＜0．05）。与模型组对比各电针组血流动力学均有明显改善。结论：同时电针曲池穴＋神门穴＋足三里穴可明显抑制胸主动脉缩窄法所引起的心肌肥厚，其机制可能是通过改善心脏血流动力学、以及促进Bcl－2的表达同时抑制Bax的表达进而抑制心肌细胞的凋亡，从而产生延缓及抑制心肌肥厚进展的作用。
目的：探討電針麯池穴、神門穴、足三裏穴對心肌肥厚模型大鼠Bcl－2及Bax影響。方法：50隻Wistar大鼠隨機分為模型組、假手術組、麯池＋足三裏組、神門＋足三裏組、麯池＋神門＋足三裏組，每組10隻，胸主動脈縮窄法建立大鼠心肌肥厚模型，電針組連續治療14天，通過實時定量PCR及West-ern blot測定心肌組織中Bcl－2、Bax mRNA及蛋白的錶達水平。結果：麯池＋足三裏組、神門＋足三裏組、麯池＋神門＋足三裏組Bcl－2及Bax mRNA及蛋白的錶達水平在治療後與模型組相比，有顯著性差異（P＜0．05）。與模型組對比各電針組血流動力學均有明顯改善。結論：同時電針麯池穴＋神門穴＋足三裏穴可明顯抑製胸主動脈縮窄法所引起的心肌肥厚，其機製可能是通過改善心髒血流動力學、以及促進Bcl－2的錶達同時抑製Bax的錶達進而抑製心肌細胞的凋亡，從而產生延緩及抑製心肌肥厚進展的作用。
목적：탐토전침곡지혈、신문혈、족삼리혈대심기비후모형대서Bcl－2급Bax영향。방법：50지Wistar대서수궤분위모형조、가수술조、곡지＋족삼리조、신문＋족삼리조、곡지＋신문＋족삼리조，매조10지，흉주동맥축착법건립대서심기비후모형，전침조련속치료14천，통과실시정량PCR급West-ern blot측정심기조직중Bcl－2、Bax mRNA급단백적표체수평。결과：곡지＋족삼리조、신문＋족삼리조、곡지＋신문＋족삼리조Bcl－2급Bax mRNA급단백적표체수평재치료후여모형조상비，유현저성차이（P＜0．05）。여모형조대비각전침조혈류동역학균유명현개선。결론：동시전침곡지혈＋신문혈＋족삼리혈가명현억제흉주동맥축착법소인기적심기비후，기궤제가능시통과개선심장혈류동역학、이급촉진Bcl－2적표체동시억제Bax적표체진이억제심기세포적조망，종이산생연완급억제심기비후진전적작용。
Objective:To discuss the effect of electroacupuncture at Quchi, Shenmen and Zusanli on Bcl-2 and Bax in rats with cardiac hypertrophy.Methods:Fifty Wistar rats were randomly divided into five groups ( a model group,a sham operation group,a Quchi & Zusanli group,a Shenmen & Zusanli group and a Quchi &Shenmen &Zusanli group) , including ten rats in each group.The rat models with cardiac hypertrophy were prepared and rats in electroacupuncture groups received a continuous wave for the treatment of 2 weeks. Through the method of real time PCR and western blot, the expression levels of Bcl-2 and Bax mRNA and protein in heart tissue were detected.Results:After treatment,the expression levels of Bcl-2 and Bax mRNA and protein in heart tissue were significantly different in Quchi & Zusanli group, Shenmen & Zusanli group and Quchi & Shenmen&Zusanli group compared with model group(P<0.05).The hemodynamics of elec-troacupuncture groups improved significantly compared with model group.Conclusion: Electroacupuncture at Quchi &Shenmen&Zusanli acupoints can prevent the myocardial hypertrophy.The mechanism may be by im-proving cardiac hemodynamics and promoting the expression of Bcl-2 and inhibiting the expression of Bax to inhibit the apoptosis of myocardial cells,then playing a role in delaying and inhibiting the process of myocardi-al hypertrophy.
