CAJ | 학술논문

以多花勾儿茶果实、叶及自制绿茶为原料，采用水浴法提取儿茶素，研究其最佳提取工艺．提取液经石油醚脱脂溶性色素后，采用大孔吸附树脂D941进行吸附分离，静态吸附和解析条件研究得出纯净水洗脱效果较好．多花勾儿茶果实、叶及自制绿茶吸附分离的纯化液，用色谱柱：Boston Green ODS PC18（4．6×250 mm ，5μm），检测波长279 nm ，流动相乙腈0．4％磷酸（w （乙腈）∶ w （0．4％磷酸）＝13∶87），柱温35℃，流速1 mL／min ，一次进样10μL来进行高效液相色谱（HPLC）检测，其结果显示，多花勾儿茶果实中儿茶素含量最低，自制绿茶中的儿茶素含量最高．
이다화구인다과실、협급자제록다위원료，채용수욕법제취인다소，연구기최가제취공예．제취액경석유미탈지용성색소후，채용대공흡부수지D941진행흡부분리，정태흡부화해석조건연구득출순정수세탈효과교호．다화구인다과실、협급자제록다흡부분리적순화액，용색보주：Boston Green ODS PC18（4．6×250 mm ，5μm），검측파장279 nm ，류동상을정0．4％린산（w （을정）∶ w （0．4％린산）＝13∶87），주온35℃，류속1 mL／min ，일차진양10μL래진행고효액상색보（HPLC）검측，기결과현시，다화구인다과실중인다소함량최저，자제록다중적인다소함량최고．
In this study ,the extraction process of catechins was investigated with a certain concentration of ethanol as solvent from man‐made green tea ,fruits and leaves of Berchemia floribunda ,using the method of waterbath .After the fat soluble pigment was removed by petroleum ether from extracting solution ,the catechins was purified by porous resin adsorption method .The static adsorption and desorption tests of catechin with macroporous resin showed that D941 was the optimal resin for purification and the pure water was the optimum eluant .Purified liquid of fruit ,leaves and home‐made green tea extracts were de‐tected by HPLC .The catechins was separated on PC18 chromatographic column (4 .6 × 250 mm ,5 μm) at 35 ℃ ,with nitrile‐0 .4% phosphoric acid (13∶87) as mobile phase at a flow rate of 1 mL/min ,and de‐tected at the wavelength of 279 nm (UV scan) .The results showed that the catechin content of fruit was the lowest ,while the content of man‐made green tea was significantly higher than that of both the fruit and leaves through the optimized extraction processes .