CAJ | 학술논문

目的：研究红芪总黄酮对大鼠离心运动后骨骼肌抗氧化能力的影响。方法：72只Wistar大鼠随机分为安静对照组（ Control group，CG）、离心运动组（ Eccentric Group，EG）和离心运动＋药物预处理组（Drug pretreatment group，DPR）。依据运动后时间点不同再次分为运动前1 h和运动后1，24，48 h 4个亚组，每组6只。离心运动前3天，药物预处理组大鼠每日定时给予0．2mg／kg红芪总黄酮灌胃，其余各组大鼠给予等量生理盐水灌胃。力竭运动后，取各组大鼠腓肠肌，分别检测乳酸脱氢酶（LDH）、活性氧（ROS）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。结果：离心运动后，大鼠骨骼肌组织中LDH，ROS和MDA含量与运动后明显升高，而SOD活性下降（ P＜0．01～0．05）。红芪总黄酮可延缓离心运动后大鼠骨骼肌ROS、MDA及LDH含量的积累并提高SOD活性，与相应对照组比较，差异具有显著性（ P＜0．05）。结论：红芪总黄酮可有效防止高强度运动后氧化应激所导致的骨骼肌损伤。
목적：연구홍기총황동대대서리심운동후골격기항양화능력적영향。방법：72지Wistar대서수궤분위안정대조조（ Control group，CG）、리심운동조（ Eccentric Group，EG）화리심운동＋약물예처리조（Drug pretreatment group，DPR）。의거운동후시간점불동재차분위운동전1 h화운동후1，24，48 h 4개아조，매조6지。리심운동전3천，약물예처리조대서매일정시급여0．2mg／kg홍기총황동관위，기여각조대서급여등량생리염수관위。력갈운동후，취각조대서비장기，분별검측유산탈경매（LDH）、활성양（ROS）、병이철（MDA）함량급초양화물기화매（SOD）활성。결과：리심운동후，대서골격기조직중LDH，ROS화MDA함량여운동후명현승고，이SOD활성하강（ P＜0．01～0．05）。홍기총황동가연완리심운동후대서골격기ROS、MDA급LDH함량적적루병제고SOD활성，여상응대조조비교，차이구유현저성（ P＜0．05）。결론：홍기총황동가유효방지고강도운동후양화응격소도치적골격기손상。
Objective:To investigate the antioxidative effect of total flavonoids of Hedysarum polybotry on skeletal muscle of rats after eccentric exercise.Methods:Wistar rats were randomly divided into a control group,an eccentric exercise group,a drug pretreatment plus eccentric exercise group.All groups were then randomized into 1h subgroup before exer-cise,1h,24h and 48 h subgroups after exercise respectively,with 6 rats in each subgroup.Rats in the drug pretreatment plus eccentric exercise group were administered by oral total flavonoids of Hedysarum polybotry ( 0 .2 mg/kg ) , once a day.Rats,gastrocnemius muscles in all groups were obtained at different time points.Lactate dehydrogenase ( LDH) , malondialdehyde ( MDA) , reactive oxygen species ( ROS) and superoxide dismutase ( SOD) in the gastrocnemius mus-cles were measured by spectroscopy, respectively.Results: After eccentric exercise, the contents of LDH, MDA and ROS were significantly increased in rats,gastrocnemius muscles (P<0.05), and gradually increased over time after ex-ercise.SOD was significantly decreased over time after exercise (P<0.05).Total flavonoids of Hedysarum polybotry could decrease the accumulation of LDH, MDA and ROS, and enhance activity of SOD in rats,gastrocnemius muscles. Conclusion:Total flavonoids of Hedysarum polybotry could prevent skeletal muscle injury caused by oxidative stress after high intensity exercise.