中医药学报
中醫藥學報
중의약학보
ACTA CHINESE MEDICINE AND PHARMACOLOGY
2015年
3期
13-16
,共4页
孙黎明%梁军%王杰姝%郭信东%夏永刚%匡海学
孫黎明%樑軍%王傑姝%郭信東%夏永剛%劻海學
손려명%량군%왕걸주%곽신동%하영강%광해학
护肝片%HPLC%荧光检测器%联苯环辛二烯型木脂素
護肝片%HPLC%熒光檢測器%聯苯環辛二烯型木脂素
호간편%HPLC%형광검측기%련분배신이희형목지소
Hugan tablets%HPLC%Fluorescence Detection%Dibenzo ( a,c) cyclooctene Lignans
目的:建立同时测定护肝片中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素和五味子丙素含量的HPLC-FLD方法。方法:用Cosmosil C18(4.6mm ×250mm,5μm)色谱柱,甲醇(A)-水(B)(85:15)等度洗脱;流速:0.6mL/min;柱温:35℃;进样量5μL,检测波长:254nm和330nm分别为激发波长和发射波长。结果:5个活性成分分离度良好,各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(R2≥0.9990);加样回收率在84.21%~105.04%范围内;RSD值在1.17%~2.18%范围内。结论:本研究建立的方法简单可靠,具有良好的精密度、重现性和稳定性,可用于护肝片中主要活性成分的同时定量分析,为不同产地、不同批次的护肝片的质量评价和质量控制提供了科学有效的依据。
目的:建立同時測定護肝片中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素和五味子丙素含量的HPLC-FLD方法。方法:用Cosmosil C18(4.6mm ×250mm,5μm)色譜柱,甲醇(A)-水(B)(85:15)等度洗脫;流速:0.6mL/min;柱溫:35℃;進樣量5μL,檢測波長:254nm和330nm分彆為激髮波長和髮射波長。結果:5箇活性成分分離度良好,各成分質量濃度與峰麵積在測定範圍內均呈良好的線性關繫(R2≥0.9990);加樣迴收率在84.21%~105.04%範圍內;RSD值在1.17%~2.18%範圍內。結論:本研究建立的方法簡單可靠,具有良好的精密度、重現性和穩定性,可用于護肝片中主要活性成分的同時定量分析,為不同產地、不同批次的護肝片的質量評價和質量控製提供瞭科學有效的依據。
목적:건립동시측정호간편중오미자순갑、오미자순을、오미자갑소、오미자을소화오미자병소함량적HPLC-FLD방법。방법:용Cosmosil C18(4.6mm ×250mm,5μm)색보주,갑순(A)-수(B)(85:15)등도세탈;류속:0.6mL/min;주온:35℃;진양량5μL,검측파장:254nm화330nm분별위격발파장화발사파장。결과:5개활성성분분리도량호,각성분질량농도여봉면적재측정범위내균정량호적선성관계(R2≥0.9990);가양회수솔재84.21%~105.04%범위내;RSD치재1.17%~2.18%범위내。결론:본연구건립적방법간단가고,구유량호적정밀도、중현성화은정성,가용우호간편중주요활성성분적동시정량분석,위불동산지、불동비차적호간편적질량평개화질량공제제공료과학유효적의거。
Objective:To establish a simultaneous determination of five markers, namely schisandrol A and B, schisan-drin A-C for evaluation and quality control of Hugan tablets by high performance liquid chromatography (HPLC) com-bined with fluorescence detection ( FLD) .Methods:The simultaneous HPLC-FLD analysis of Hugan tablets was recor-ded at excitation wavelength of 254nm and emission wavelength of 330nm by using a reversed phase C18 column (4.6mm ×250mm, 5 μm) by an isocratic elution mobile phase consisting of methanol and water (85:15) within 25min at 35℃with a flow rate of 0.6mL/min.Results: In the quantitative analysis, all calibration curves of five selected markers showed good linear regression ( R2≥0.999 0) within the tested ranges, and the recovery of the method was in the range of 84.21%~105.04%, with RSD values ranging from 1.17%to 2.18%.Conclusion:The results demonstrate that the developed method is a rapid and effective analytical method for the quality assessment of Hugan tablets.