中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2015年
11期
1639-1642,1643
,共5页
结直肠肿瘤%TNF 相关凋亡诱导配体%信号传导
結直腸腫瘤%TNF 相關凋亡誘導配體%信號傳導
결직장종류%TNF 상관조망유도배체%신호전도
Colorectal Neoplasms%TNF -Related Apoptosis -Inducing Ligand%Signal Transduction
目的：探讨肿瘤坏死因子相关诱导凋亡配体（TRAIL）信号通路在结直肠癌发病机制中的作用。方法取41例结直肠癌组织（结直肠癌组）和癌旁正常组织（正常组），免疫组化法测定两组组织中TRAIL 及 TRAILR3、TRAILR4蛋白的表达。结果结直肠癌组织 TRAIL、TRAILR3的阳性率与正常组差异均无统计学意义（88％与90％；95％与93％，χ2＝0．125、0．213，均 P ＞0．05），但两者的阳性表达强度显著低于正常组[（0．237±0．036）与（0．289±0．069）；（0．226±0．052）与（0．281±0．068），t ＝4．125、4.025，均 P ＜0.01]；两者的阳性表达强度在低分化癌组织中显著低于高中分化癌[（0．205±0．021）与（0．245±0．034）；（0．185±0．032）与（0．236±0．051），t ＝4．025、2．664，P ＜0．01、P ＜0．05]，但与淋巴结转移无关[（0．235±0.035）与（0．241±0．338）；（0．216±0．048）与（0．231±0．054），t ＝0.448、0．862，均 P ＞0．05]。结直肠癌组TRAILR4蛋白的阳性率和阳性表达强度与正常组差异均无统计学意义[93％与98％；（0．196±0．085）与（0.219±0．061），χ2＝1．051、t ＝1．353，均 P ＞0．05]，还与癌分化程度、淋巴结转移无关[（0．176±0．052）与（0．201±0．091）；（0．194±0．054）与（0．197±0．100），t ＝0．667、0．448，均 P ＞0．05]。结论TRAIL 和TRAILR3在结直肠癌组织中表达下降，且与 Gleason 分级相关，它们可作为结直肠癌的预后指标。
目的：探討腫瘤壞死因子相關誘導凋亡配體（TRAIL）信號通路在結直腸癌髮病機製中的作用。方法取41例結直腸癌組織（結直腸癌組）和癌徬正常組織（正常組），免疫組化法測定兩組組織中TRAIL 及 TRAILR3、TRAILR4蛋白的錶達。結果結直腸癌組織 TRAIL、TRAILR3的暘性率與正常組差異均無統計學意義（88％與90％；95％與93％，χ2＝0．125、0．213，均 P ＞0．05），但兩者的暘性錶達彊度顯著低于正常組[（0．237±0．036）與（0．289±0．069）；（0．226±0．052）與（0．281±0．068），t ＝4．125、4.025，均 P ＜0.01]；兩者的暘性錶達彊度在低分化癌組織中顯著低于高中分化癌[（0．205±0．021）與（0．245±0．034）；（0．185±0．032）與（0．236±0．051），t ＝4．025、2．664，P ＜0．01、P ＜0．05]，但與淋巴結轉移無關[（0．235±0.035）與（0．241±0．338）；（0．216±0．048）與（0．231±0．054），t ＝0.448、0．862，均 P ＞0．05]。結直腸癌組TRAILR4蛋白的暘性率和暘性錶達彊度與正常組差異均無統計學意義[93％與98％；（0．196±0．085）與（0.219±0．061），χ2＝1．051、t ＝1．353，均 P ＞0．05]，還與癌分化程度、淋巴結轉移無關[（0．176±0．052）與（0．201±0．091）；（0．194±0．054）與（0．197±0．100），t ＝0．667、0．448，均 P ＞0．05]。結論TRAIL 和TRAILR3在結直腸癌組織中錶達下降，且與 Gleason 分級相關，它們可作為結直腸癌的預後指標。
목적：탐토종류배사인자상관유도조망배체（TRAIL）신호통로재결직장암발병궤제중적작용。방법취41례결직장암조직（결직장암조）화암방정상조직（정상조），면역조화법측정량조조직중TRAIL 급 TRAILR3、TRAILR4단백적표체。결과결직장암조직 TRAIL、TRAILR3적양성솔여정상조차이균무통계학의의（88％여90％；95％여93％，χ2＝0．125、0．213，균 P ＞0．05），단량자적양성표체강도현저저우정상조[（0．237±0．036）여（0．289±0．069）；（0．226±0．052）여（0．281±0．068），t ＝4．125、4.025，균 P ＜0.01]；량자적양성표체강도재저분화암조직중현저저우고중분화암[（0．205±0．021）여（0．245±0．034）；（0．185±0．032）여（0．236±0．051），t ＝4．025、2．664，P ＜0．01、P ＜0．05]，단여림파결전이무관[（0．235±0.035）여（0．241±0．338）；（0．216±0．048）여（0．231±0．054），t ＝0.448、0．862，균 P ＞0．05]。결직장암조TRAILR4단백적양성솔화양성표체강도여정상조차이균무통계학의의[93％여98％；（0．196±0．085）여（0.219±0．061），χ2＝1．051、t ＝1．353，균 P ＞0．05]，환여암분화정도、림파결전이무관[（0．176±0．052）여（0．201±0．091）；（0．194±0．054）여（0．197±0．100），t ＝0．667、0．448，균 P ＞0．05]。결론TRAIL 화TRAILR3재결직장암조직중표체하강，차여 Gleason 분급상관，타문가작위결직장암적예후지표。
Objective To investigate the potential roles of tumor necrosis factor -related apoptosis -indu-cing ligand(TRAIL)signaling system in the pathogenesis of colorectal cancer.Methods Immunohistochemistry tech-niques were used,the TRAIL and its receptor(TRAILR3,TRAILR4)protein were analyzed in both 41 colorectal cancer samples(colorectal cancer group)and normal samples beside cancer tissue(normal group).Results The lev-els of TRAIL and TRAILR3 protein expression in the colorectal cancer group were significantly lower than those in the normal group[(0.237 ±0.036)vs.(0.289 ±0.069);(0.226 ±0.052)vs.(0.281 ±0.068),t =4.125,4.025,all P <0.01],while the positive rate of them were nonsignificant difference between the two groups(88% vs.90%;95%vs.93%,χ2 =0.125,0.213,all P >0.05).The expression levels of them in the colorectal cancer group with poorl differentiation were notably lower than those with high -moderate differentiation[(0.205 ±0.021 )vs (0.245 ± 0.034);(0.185 ±0.032)vs (0.236 ±0.051),t =4.025,2.664,P <0.01,P <0.05],but they were not strongly correlated to lymph nodes metastasis[(0.235 ±0.035 )vs (0.241 ±0.338);(0.216 ±0.048)vs (0.231 ± 0.054),t =0.448,0.862,all P >0.05].Moreover,the positive rate and expression levels of TRAILR4 protein was non -statistic significant difference between the two groups[93% vs 98%;(0.196 ±0.085)vs (0.219 ±0.061),χ2 =1.051,t =1.353,all P >0.05],and it was also non -significantly correlated with cancer cell differentiation and lymph nodes metastasis[(0.176 ±0.052)vs (0.201 ±0.091);(0.194 ±0.054)vs (0.197 ±0.100),t =0.667, 0.448,all P >0.05].Conclusion The levels of TRAIL and TRAILR3 expression are attenuated at colorectal cancer tissue.The expression of them are correlated with cancer cell differentiation grade.These findings indicate that TRAIL system may be associated with the malignant phenotype in colorectal cancer.
