CAJ | 학술논문

为了研究木瓜蛋白酶在[Cnmim]BF4-NaH2PO4双水相体系中的分配行为，在298.15 K条件下利用浊点法测定了[Cnmim]BF4-NaH2PO4双水相体系的双节线和液液相平衡数据，建立了木瓜蛋白酶在该体系中的三维分配模型。利用Merchuk方程、Othmer-Tobias方程和Bancroft方程分别关联了双节线数据、液液相平衡数据，拟合度分别在0.975、0.994以上，结果满意。木瓜蛋白酶分配系数的对数与该体系上相的离子液体浓度和下相的盐浓度相关度都较高，故通过Matlab建立了三者之间的分配模型，实验值和预测值之间的相对偏差均在5%以内，表明该模型能有效地预测木瓜蛋白酶在[Cnmim]BF4-NaH2PO4双水相体系中的分配行为。
위료연구목과단백매재[Cnmim]BF4-NaH2PO4쌍수상체계중적분배행위，재298.15 K조건하이용탁점법측정료[Cnmim]BF4-NaH2PO4쌍수상체계적쌍절선화액액상평형수거，건립료목과단백매재해체계중적삼유분배모형。이용Merchuk방정、Othmer-Tobias방정화Bancroft방정분별관련료쌍절선수거、액액상평형수거，의합도분별재0.975、0.994이상，결과만의。목과단백매분배계수적대수여해체계상상적리자액체농도화하상적염농도상관도도교고，고통과Matlab건립료삼자지간적분배모형，실험치화예측치지간적상대편차균재5%이내，표명해모형능유효지예측목과단백매재[Cnmim]BF4-NaH2PO4쌍수상체계중적분배행위。
To investigate the partition behavior of papain in the ionic liquids aqueous two-phase system containing [Cnmim]BF4+NaH2PO4, the binodal curve and liquid-liquid equilibrium was measured by means of cloud point at T=298.15 K and a 3D theoretical model was established. The data fitting of binodal and the equilibrium was based on Merchuk, Othmer-Tobias and Bancroft equations with a high degree of fitting of above 0.975 and 0.994, respectively. Then, the correlation between logarithm of the partition coefficient and both the concentration of ionic liquids in upper phase and the salt concentration in lower phase was studied, and a partition model was established by Matlab. The mean relative deviations between the experimental and calculated data were <5%, which was sufficient to prove the reliability of this model.