电子测量与仪器学报
電子測量與儀器學報
전자측량여의기학보
JOURNAL OF ELECTRONIC MEASUREMENT AND INSTRUMENT
2015年
5期
662-668
,共7页
高跃明%李天麒%林传阳%潘少恒%韦孟宇%杜民
高躍明%李天麒%林傳暘%潘少恆%韋孟宇%杜民
고약명%리천기%림전양%반소항%위맹우%두민
免疫层析%定量检测%特征量%基线漂移
免疫層析%定量檢測%特徵量%基線漂移
면역층석%정량검측%특정량%기선표이
immune-chromatographic%quantitative detection%characteristic value%baseline drift
荧光免疫层析定量检测技术是一种正在兴起的即时检验技术。在前期荧光免疫层析定量检测仪的研究基础上,采用小波算法对A/D采样后的荧光信号进行去噪处理,选用中值滤波剔除基线漂移,最后比较了采用测试线峰值和面积作为特征量的定量检测效果。数据显示,去噪后信噪比提高10 dB,相对均方差减小30.4%;采用峰值和面积值作为定量特征量的测量CV值分别为5.1%和0.4%,测量误差分别为9.1%和0.9%。结果表明采用积分面积作为特征量对荧光信号进行定量检测具有更好的准确度和重复性。
熒光免疫層析定量檢測技術是一種正在興起的即時檢驗技術。在前期熒光免疫層析定量檢測儀的研究基礎上,採用小波算法對A/D採樣後的熒光信號進行去譟處理,選用中值濾波剔除基線漂移,最後比較瞭採用測試線峰值和麵積作為特徵量的定量檢測效果。數據顯示,去譟後信譟比提高10 dB,相對均方差減小30.4%;採用峰值和麵積值作為定量特徵量的測量CV值分彆為5.1%和0.4%,測量誤差分彆為9.1%和0.9%。結果錶明採用積分麵積作為特徵量對熒光信號進行定量檢測具有更好的準確度和重複性。
형광면역층석정량검측기술시일충정재흥기적즉시검험기술。재전기형광면역층석정량검측의적연구기출상,채용소파산법대A/D채양후적형광신호진행거조처리,선용중치려파척제기선표이,최후비교료채용측시선봉치화면적작위특정량적정량검측효과。수거현시,거조후신조비제고10 dB,상대균방차감소30.4%;채용봉치화면적치작위정량특정량적측량CV치분별위5.1%화0.4%,측량오차분별위9.1%화0.9%。결과표명채용적분면적작위특정량대형광신호진행정량검측구유경호적준학도화중복성。
Immunofluorescence chromatographic quantitative detection technology is an emerging technique of the point of care test (POCT).Based on the previous work of immunofluorescence quantitative chromatography detector, the wavelet algorithm was used to denoise the fluorescence signal after the A/D sampling in this paper.Then the baseline drift was removed by the median filter method.Finally, the peak height and the area were sellected as the characteristic value for the quantitative detection.The experimental data shown that the SNR improved 10dB and the SRMS reduced by 30.4%after denoising.In the quantitatively detect step, the CV were 5.1% and 0.4%, and the test error were 9.1%and 0.9%, for the peak hight and area characteristic value, respectively.The results show that the peak area of the test line as the characteristic value has better accuracy and repeatability than the peak height .