CAJ | 학술논문

目的：探讨慢性运动性中枢疲劳大鼠海马5-羟色胺(5-HT)与血清中色氨酸和支链氨基酸的含量变化特点。方法建立慢性运动性中枢疲劳大鼠模型，采用高效液相色谱-质谱方法测定模型大鼠的血清色氨酸和支链氨基酸水平，以及海马组织的5-HT含量。结果慢性运动性中枢疲劳大鼠血清色氨酸水平明显降低；3种支链氨基酸的测定水平虽差异无统计学意义，但其含量与色氨酸的比值均出现明显升高。结论慢性运动性中枢疲劳大鼠血清色氨酸水平的降低有利于支链氨基酸进入中枢，该机制可能影响中枢5-HT含量的变化。
목적：탐토만성운동성중추피로대서해마5-간색알(5-HT)여혈청중색안산화지련안기산적함량변화특점。방법건립만성운동성중추피로대서모형，채용고효액상색보-질보방법측정모형대서적혈청색안산화지련안기산수평，이급해마조직적5-HT함량。결과만성운동성중추피로대서혈청색안산수평명현강저；3충지련안기산적측정수평수차이무통계학의의，단기함량여색안산적비치균출현명현승고。결론만성운동성중추피로대서혈청색안산수평적강저유리우지련안기산진입중추，해궤제가능영향중추5-HT함량적변화。
Aim To analyze the relationship of hippocampus serotonin with alternations of serum tryptophan and branched chain amino acids (BCAA) on the basis of improved Rats’ Chronic Exercise-induced Central Fatigue Model.Methods① Rats’ Chronic Exercise-induced Central Fatigue Model was established.② The method of HPLC-MC to measure the level of tryptophan and BCAA in rat’s serum was adopted and the relationship between them was analyzed.③ The method of HPLC-ECD to measure the content of 5-HT in rat hippocampus was used.Results It was easily found that there was a signiifcant decrease of serum tryptophan in rats with central fatigue after running (P<0.01), compared with the control group, while BCAA (serum leucine, isoleucine and valine), the rival competitor of tryptophan when entered into the central nervous system, were not signiifcantly changed. Ratios of leucine, isoleucine and valine to tryptophan were also signiifcantly increased (P<0.01), and the ratio of sum of BCAA to tryptophan as well. Conclusion Rats’ level of 5-HT had a sharp increase with both chronic central fatigue after exercise and after one-time exhaustive exercise. But there was some difference in regulation mechanism between them. The fall of serum tryptophan and the rise of the ratio of BCAA/TRP were favorable for BCAA to enter the central nerve. And at the same time, the measurement of tryptophan in serum was likely to become the biochemical indicators of the extreme fatigue of organism.