CAJ | 학술논문

目前酶制剂生产过程中常用的浓缩方法存在工艺复杂、能耗较高、成本高昂等缺点，并成为酶制剂工业进一步发展的关键步骤。本文综述了蒸发、超滤、吸附和冷冻等常用的酶制剂浓缩方法，以及较新的离子液体和水合物浓缩酶制剂方法等，并比较了这些方法的优缺点。蒸发浓缩酶制剂技术成熟，但是能耗较高、酶失活率高。超滤和吸附浓缩酶制剂方法能耗较低，但是膜和吸附材料成本较高、再生困难。冷冻酶制剂浓缩方法酶失活少，但是浓缩率低。离子液体酶制剂浓缩方法具有保持酶活性、易于放大、可连续操作等优点，但是技术仍不成熟。水合物酶制剂浓缩方法目前尚处于实验室研究阶段。因此，开发出工艺简单、性能可靠、能耗较低、成本低廉的高纯度酶制剂浓缩方法依然是未来的研究方向。
목전매제제생산과정중상용적농축방법존재공예복잡、능모교고、성본고앙등결점，병성위매제제공업진일보발전적관건보취。본문종술료증발、초려、흡부화냉동등상용적매제제농축방법，이급교신적리자액체화수합물농축매제제방법등，병비교료저사방법적우결점。증발농축매제제기술성숙，단시능모교고、매실활솔고。초려화흡부농축매제제방법능모교저，단시막화흡부재료성본교고、재생곤난。냉동매제제농축방법매실활소，단시농축솔저。리자액체매제제농축방법구유보지매활성、역우방대、가련속조작등우점，단시기술잉불성숙。수합물매제제농축방법목전상처우실험실연구계단。인차，개발출공예간단、성능가고、능모교저、성본저렴적고순도매제제농축방법의연시미래적연구방향。
Presently,the enzyme concentrating method as the critical procedure in production of enzyme has many disadvantages,including complex process and high energy consumption. The common methods,including evaporation,ultrafiltration,adsorption,freezing are compared with the latest methods,including ionic liquid,hydrate,and their advantages and disadvantages are analyzed. Evaporation technology is mature but consumes excessive energy and keeps less enzyme activity. Ultrafiltration and adsorption consume less energy but have high material costs and are difficult to regenerate. The disadvantage of freezing concentration is the limit of concentration,though it can keep more activity. Concentrating by the ionic liquid method is easy to scale up and has the advantage of continuous operation,but the technology is not mature enough. The hydrate method is still in the laboratory. So developing a method of simple process,reliable performance,low energy consumption and low cost will be a research direction in the future.