CAJ | 학술논문

目的：探讨流式细胞术检测支气管肺泡灌洗液(BALF)淋巴细胞免疫表型的临床意义。方法应用流式细胞仪双色直接免疫荧光法对15例肺癌(肺癌组)和20例肺良性病变患者(良性病变组)的支气管肺泡灌洗液T淋巴细胞亚群进行分析。结果肺癌组肺泡灌洗液中CD3+、CD3+CD4+、 CD4+/CD8+比值均低于良性病变组，差异有统计学意义(P<0.05)，肺癌组肺泡灌洗液中CD3+CD8+高于良性病变组，差异有统计学意义(P<0.05)。结论肺部恶性肿瘤细胞免疫功能低下，可以通过流式细胞术检测肺泡灌洗液淋巴细胞免疫表型，为临床诊断肺部良、恶性病变提供有效依据。
목적：탐토류식세포술검측지기관폐포관세액(BALF)림파세포면역표형적림상의의。방법응용류식세포의쌍색직접면역형광법대15례폐암(폐암조)화20례폐량성병변환자(량성병변조)적지기관폐포관세액T림파세포아군진행분석。결과폐암조폐포관세액중CD3+、CD3+CD4+、 CD4+/CD8+비치균저우량성병변조，차이유통계학의의(P<0.05)，폐암조폐포관세액중CD3+CD8+고우량성병변조，차이유통계학의의(P<0.05)。결론폐부악성종류세포면역공능저하，가이통과류식세포술검측폐포관세액림파세포면역표형，위림상진단폐부량、악성병변제공유효의거。
Objective To investigate clinical significance of flow cytometry for lymphocyte immunophenotyping detection in bronchial alveolar lavage fluid (BALF).MethodsFlow cytometry double color direct immunofluorescence method was applied in analysis of T lymphocytes subset in bronchial alveolar lavage fluid in 15 lung cancer cases (lung cancer group) and 20 lung benign lesion cases (benign lesion group). Results The lung cancer group had lower values of CD3+, CD3+CD4+, CD4+/CD8+ ratio in alveolar lavage fluid than the benign lesion group, and their difference had statistical significance (P<0.05). The lung cancer group had higher CD3+CD8+ value than the benign lesion group, and their difference had statistical significance (P<0.05). Conclusion The immunity function in lung cancer patients significantly decreases, and their lymphocyte immunophenotyping in alveolar lavage fluid can detected by flow cytometry, for providing effective reference for clinical diagnosis of benign and malignant lesion.