CAJ | 학술논문

目的：观察复方红景天喂食2型糖尿病肾病大鼠尿微量白蛋白（ mALB ）和血晚期氧化蛋白产物（ AOPPs）的变化，并探究其保护肾脏的作用机制。方法将SPF级8周龄雄性Wistar大鼠50只随机分为正常对照组5只和模型组45只。将成功制作的43只糖尿病肾病模型再随机分为模型对照组10只、小剂量红景天组11只、中剂量红景天组11只、大剂量红景天组11只。小、中、大剂量红景天组分别给予复方红景天3、6、12 g／（kg· d ），模型对照组给予等体积生理盐水，连续灌胃4周。治疗前后尾静脉取血检测FBG、FINS、HOMR-IR、TG、TC、AOPPs及12 h尿mALB水平，并观察大鼠体质量（ BW）的变化。结果治疗前，糖尿病肾病模型大鼠BW低于正常对照组，FPG、FINS、HOMR-IR、TG、TC、mALB、AOPPs水平均高于正常对照组（ P＜0．05或＜0．01）。治疗4周后，小、中、大剂量红景天组BW均高于模型对照组，FPG、AOPPs、mALB水平低于模型对照组（ P均＜0．01），小、中、大剂量红景天组BW、AOPPs、mALB水平比较差异无统计学意义（P均＞0．05）。结论复方红景天可能通过降低T2DN大鼠尿mALB、血AOPPs水平，从而发挥肾脏保护作用。
목적：관찰복방홍경천위식2형당뇨병신병대서뇨미량백단백（ mALB ）화혈만기양화단백산물（ AOPPs）적변화，병탐구기보호신장적작용궤제。방법장SPF급8주령웅성Wistar대서50지수궤분위정상대조조5지화모형조45지。장성공제작적43지당뇨병신병모형재수궤분위모형대조조10지、소제량홍경천조11지、중제량홍경천조11지、대제량홍경천조11지。소、중、대제량홍경천조분별급여복방홍경천3、6、12 g／（kg· d ），모형대조조급여등체적생리염수，련속관위4주。치료전후미정맥취혈검측FBG、FINS、HOMR-IR、TG、TC、AOPPs급12 h뇨mALB수평，병관찰대서체질량（ BW）적변화。결과치료전，당뇨병신병모형대서BW저우정상대조조，FPG、FINS、HOMR-IR、TG、TC、mALB、AOPPs수평균고우정상대조조（ P＜0．05혹＜0．01）。치료4주후，소、중、대제량홍경천조BW균고우모형대조조，FPG、AOPPs、mALB수평저우모형대조조（ P균＜0．01），소、중、대제량홍경천조BW、AOPPs、mALB수평비교차이무통계학의의（P균＞0．05）。결론복방홍경천가능통과강저T2DN대서뇨mALB、혈AOPPs수평，종이발휘신장보호작용。
Objective To observe the changes of microalbuminuria ( mALB) and advanced oxidation protein products ( AOPPs ) of rats with type 2 diabetic nephropathy ( T2DN) by feeding compound rhodiola sachalinensis ( Fufang Hongjin-tian) and to investigate its mechanism of protecting the kidney.Methods Fifty male 8-week-old Wistar rats were random-ly divided into the normal control group (A group, n=5) and model group (n=45).During the 45 rats, 43 rat models with DN were successfully made.Then, they were randomly divided into the model control group (group B, n=10), low dose compound rhodiola sachalinensis group ( group C) , medium-dose compound rhodiola sachalinensis group ( group D, n=11), and high-dose compound rhodiola sachalinensis group (group E, n=11).Rats in the groups C, D and E were treated by 3, 6 and 12 g/( kg· d) compound rhodiola sachalinensis and rats in the group B was treated by intragastric ad-ministration of the same amount of normal saline for 4 weeks.The FBG, FINS, HOMR-IR, TG, TC, AOPPs and 12-hour mALB level in the caudal vein of rats were detected before and after treatment and the body weight ( BW) was also ob-served.Results Before treatment, the BW of group B was significantly lower than that of group A, but FPG, FINS, HOMR-IR, TG, TC, mALB and AOPPs level was higher than that of group A (P<0.05 or P<0.01).After treatment of 4 weeks, the BW of groups C, D and E was higher than that of group B, but FPG, AOPPs and mALB was lower than that of the group B (all P<0.01).No statistical difference was found in FPG, AOPPs and mALB among groups C, D and E (all P>0.05).Conclusion Compound rhodiola sachalinensis (Fufang Hongjingtian) has the protective effect on kidney of T2DN rats by reducing the levels of mALB and AOPPs.