CAJ | 학술논문

目的：明确乙醇联合亚低温治疗对心肺复苏后家兔脑组织的协同保护作用。方法通过体外致颤法建立家兔心肺复苏模型，80只家兔随机分为五组：假手术组（ A组）、常规复苏组（B组）、局部亚低温组（C组）、乙醇组（D组）、乙醇联合局部亚低温组（E组），每组16只。 A组家兔常温下行麻醉、手术、气管切开并插入T型气管套管但不致颤；B组致颤并行常规复苏；C组自主循环恢复（ ROSC）即刻给予局部亚低温；D组ROSC即刻给予1．5 g／kg乙醇（用0．9％盐水稀释至10 mL）微量泵颈内静脉泵入（15 min内）；E组ROSC后即刻给予1．5 g／kg乙醇（用0．9％盐水稀释至10 mL）微量泵颈内静脉泵入（15 min内）＋局部亚低温。各组于实验前、实验结束后12 h测定血清S100β蛋白含量，并于实验结束后12 h处死动物，测定脑组织含水量、脑坏死体积。结果①血清中S100β蛋白含量：与A组比较，其余四组该指标明显增高（P＜0．05），其中E组升高幅度明显低于B、C、D组（P＜0．05）；②脑组织含水量、脑坏死体积：与A组比较，其余四组脑组织含水量明显增多（P＜0．05），脑坏死体积明显增大（P＜0．05），其中E组脑组织含水量增多幅度、脑坏死体积增大幅度均明显低于B、C、D组（P＜0．05）。结论乙醇联合亚低温治疗对心脏骤停（ CA）后脑组织具有协同保护作用。
목적：명학을순연합아저온치료대심폐복소후가토뇌조직적협동보호작용。방법통과체외치전법건립가토심폐복소모형，80지가토수궤분위오조：가수술조（ A조）、상규복소조（B조）、국부아저온조（C조）、을순조（D조）、을순연합국부아저온조（E조），매조16지。 A조가토상온하행마취、수술、기관절개병삽입T형기관투관단불치전；B조치전병행상규복소；C조자주순배회복（ ROSC）즉각급여국부아저온；D조ROSC즉각급여1．5 g／kg을순（용0．9％염수희석지10 mL）미량빙경내정맥빙입（15 min내）；E조ROSC후즉각급여1．5 g／kg을순（용0．9％염수희석지10 mL）미량빙경내정맥빙입（15 min내）＋국부아저온。각조우실험전、실험결속후12 h측정혈청S100β단백함량，병우실험결속후12 h처사동물，측정뇌조직함수량、뇌배사체적。결과①혈청중S100β단백함량：여A조비교，기여사조해지표명현증고（P＜0．05），기중E조승고폭도명현저우B、C、D조（P＜0．05）；②뇌조직함수량、뇌배사체적：여A조비교，기여사조뇌조직함수량명현증다（P＜0．05），뇌배사체적명현증대（P＜0．05），기중E조뇌조직함수량증다폭도、뇌배사체적증대폭도균명현저우B、C、D조（P＜0．05）。결론을순연합아저온치료대심장취정（ CA）후뇌조직구유협동보호작용。
Objective To investigate the protect affect of ethanol with hypothermia for cardiopulmonary resuscitation ( CPR ) of rabbit brain.Methods Ventricular fibrillation ( VF ) was induced by the external electricity, eighty adult rats were randomly divided into five groups ( n=16 ):sham-operated group (group A), control group (group B), hypothermia group (group C), alcohol group ( group D ) , alcohol combined with hypothermia group ( group E ) . Group A rabbits were endotracheal intubation, anesthesia and surgery at room temperature, Group B cause cardiac arrest and parallel conventional recovery.Group C give local mild hypothermia after ROSC.Group D were given 1.5 g/kg ethanol ( Diluted with 0.9% saline to 10 mL, pumped through the internal jugular vein by micro-pump in 15 min), Group E were given 1.5 g/kg ethanol (Diluted with 0.9%saline to 10 mL, pumped through the internal jugular vein by micro-pump in 15 min) with local mild hypothermia after ROSC.Determine the serum content of protein S100βcontent of each group before the trial and 12 h after the trial.Twelve hours after the trial, the rabbits were sacrificed to determine the brain water content ,brain dead volume .Results ①The serum S100βcontent:compared with group A, group B、C、D、E showed a significantly higher serum S100βcontent, especially group B (P<0.05);and group E showed the lowest serum S100βcontent among group B、C、D、E ( P <0.05 ); ②The brain water content, brain dead volume:compared with group A, group B、C、D、E showed significantly higher brain water content and brain dead volume, especially group B (P<0.05); and group E showed the lowest brain water content, brain dead volume among group B、C、D、E (P<0.05).Conclusion Ethanol combined with hypothermia show synergic effects on cerebral resuscitation after cardiac arrest.