CAJ | 학술논문

背景与目的：有研究证实，正丁酸钠(sodium butyrate，NaB)对肿瘤细胞具有抑制增殖、诱导分化和促凋亡的作用，但对涎腺腺样囊性癌(salivary adenoid cystic carcinoma，SACC)的作用机制尚不明确。该研究主要探讨不同浓度NaB对SACC细胞株ACC-M侵袭、迁移的影响及其作用机制。方法：MTT法探索NaB作用ACC-M细胞的最佳浓度并观察细胞的生长情况，Transwell小室实验检测NaB对ACC-M细胞侵袭、迁移能力的影响，实时荧光定量PCR(real-time PCR，RT-PCR)和蛋白质印迹法(Western blot)分别检测5组浓度药物作用后ACC-M细胞中高迁移率蛋白-1(high mobility group box-1，HMGB1)、toll样受体-4(toll like receptor-4，TLR4) mRNA和蛋白的表达。结果：与对照组相比，加入NaB 0.625、1.25、2.5、5及10 mmol/L的5组均能抑制ACC-M细胞增殖且呈明显浓度依赖性(P<0.05)。5组浓度NaB均可抑制ACC-M细胞体外侵袭和迁移能力(P<0.05)，同时能降低ACC-M细胞HMGB1、TLR4 mRNA及蛋白的表达(P<0.05)；相关性分析显示TLR4蛋白表达的降低与HMGB1的抑制呈正相关(r=0.810，P<0.05)。结论：NaB可抑制ACC-M细胞增殖，显著降低ACC-M细胞的侵袭和迁移能力，同时降低HMGB1、TLR4 mRNA和蛋白的表达，且2者表达量呈明显正相关，提示NaB可能是通过下调HMGB1、TLR4 mRNA和蛋白的表达来实现对ACC-M细胞侵袭、迁移能力的抑制。
배경여목적：유연구증실，정정산납(sodium butyrate，NaB)대종류세포구유억제증식、유도분화화촉조망적작용，단대연선선양낭성암(salivary adenoid cystic carcinoma，SACC)적작용궤제상불명학。해연구주요탐토불동농도NaB대SACC세포주ACC-M침습、천이적영향급기작용궤제。방법：MTT법탐색NaB작용ACC-M세포적최가농도병관찰세포적생장정황，Transwell소실실험검측NaB대ACC-M세포침습、천이능력적영향，실시형광정량PCR(real-time PCR，RT-PCR)화단백질인적법(Western blot)분별검측5조농도약물작용후ACC-M세포중고천이솔단백-1(high mobility group box-1，HMGB1)、toll양수체-4(toll like receptor-4，TLR4) mRNA화단백적표체。결과：여대조조상비，가입NaB 0.625、1.25、2.5、5급10 mmol/L적5조균능억제ACC-M세포증식차정명현농도의뢰성(P<0.05)。5조농도NaB균가억제ACC-M세포체외침습화천이능력(P<0.05)，동시능강저ACC-M세포HMGB1、TLR4 mRNA급단백적표체(P<0.05)；상관성분석현시TLR4단백표체적강저여HMGB1적억제정정상관(r=0.810，P<0.05)。결론：NaB가억제ACC-M세포증식，현저강저ACC-M세포적침습화천이능력，동시강저HMGB1、TLR4 mRNA화단백적표체，차2자표체량정명현정상관，제시NaB가능시통과하조HMGB1、TLR4 mRNA화단백적표체래실현대ACC-M세포침습、천이능력적억제。
Background and purpose: Researches demonstrated that the butyric acid sodium salt (sodium butyrate, NaB) has effect on the inhibition of tumor cell proliferation, differentiation and apoptosis-promoting, while the mechanism on salivary adenoid cystic carcinoma(SACC) is still uncertain. This study mainly probed into the impact of different concentration of sodium butyrate on the migration and invasion of SACC cell line ACC-M, and its mechanism of action. Methods:MTT assay explored the optimal concentration of sodium butyrate on the cell ACC-M and the observation of cell growth. Transwell assay was used to detect the effects of sodium butyrate on the ACC-M cells on the aspact of invasion and migration ability. Fluorescence real-time quantitative PCR (RT-PCR) and Western blot were used to test respectively the expression of HMGB1, TLR4 mRNA and protein in ACC-M after functioned by 5 group drugs with different concentrations. Results:Compared with the control group, on the one hand, the concentration 0.625, 1.25, 2.5, 5 and 10 mmol/L of sodium butyrate could effectively inhibit cell proliferation and apparently showing concentra-tion-dependence (P<0.05);On the other hand, 5 sets concentration of sodium butyrate could also effectively inhibit invasion and migration ability of ACC-M cells in vitro (P<0.05), as well as reducing the expression of HMGB1, TLR4 mRNA and protein in ACC-M cells (P<0.05). Furthermore related analysis showed that the decline of TLR4 protein expression was positively correlated with inhibition of HMGB1 (r=0.810, P<0.05). Conclusion:Sodium butyrate has an effect on inhibiting ACC-M cell proliferation, signiifcantly reducing ACC-M cell invasion and migration capabilities, and reducing expression of HMGB1, TLR4 mRNA and protein, and both expression amount are positively correlated, Meanwhile the positively correlation suggests that sodium butyrate probably achieve the inhibition ability by lowering the expression of HMGB1, TLR4 mRNA and protein in ACC-M cell.