中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2015年
4期
269-274
,共6页
口腔肿瘤%增殖%侵袭%转移
口腔腫瘤%增殖%侵襲%轉移
구강종류%증식%침습%전이
Oral squamous cell carcinoma%Proliferation%Invasion%Migration
背景与目的：口腔颌面部恶性肿瘤中约80%以上为口腔鳞状细胞癌(oral squamous cell carcino-ma，OSCC)，虽过去的几十年诊断及治疗技术不断改进，其存活率却没有得到明显改善，5年生存率仍小于50%。该研究旨在探讨斯钙素2(stanniocalcin 2，STC2)对口腔鳞癌细胞KB的增殖、凋亡及侵袭迁移的影响。方法：构建STC2的RNA干扰载体，将其转染KB细胞使STC2基因沉默后，采用CCK8实验检测STC2对KB细胞增殖的影响，通过APC Annexin V/7-AAD染色、流式细胞术检测细胞凋亡情况，分析STC2对细胞凋亡的影响。细胞划痕和细胞小室(Transwell)试验分析比较STC2基因沉默后对口腔鳞癌细胞侵袭和迁移的差异。最后用蛋白[质]印迹法(Western blot)检测凋亡、转移相关蛋白。结果：成功构建KB细胞STC2敲除细胞系，CCK8增殖实验结果显示，STC2沉默后，细胞增殖被明显抑制，其生长速度低于对照组(P<0.001)。在顺铂诱导细胞凋亡过程中，STC2沉默后KB细胞凋亡一定程度被促进。与KB细胞相比，STC2沉默后的细胞迁移和侵袭性明显减弱。Western blot检测发现，沉默STC2后Bcl-2、促细胞迁移和侵袭蛋白Caveolin-1和β-catenin表达下调，bax表达上升。结论：STC2可能参与调控口腔鳞癌细胞KB的增殖凋亡，促进KB细胞的侵袭转移能力，同时一定程度上减弱KB对化疗药物顺铂的敏感性。
揹景與目的：口腔頜麵部噁性腫瘤中約80%以上為口腔鱗狀細胞癌(oral squamous cell carcino-ma，OSCC)，雖過去的幾十年診斷及治療技術不斷改進，其存活率卻沒有得到明顯改善，5年生存率仍小于50%。該研究旨在探討斯鈣素2(stanniocalcin 2，STC2)對口腔鱗癌細胞KB的增殖、凋亡及侵襲遷移的影響。方法：構建STC2的RNA榦擾載體，將其轉染KB細胞使STC2基因沉默後，採用CCK8實驗檢測STC2對KB細胞增殖的影響，通過APC Annexin V/7-AAD染色、流式細胞術檢測細胞凋亡情況，分析STC2對細胞凋亡的影響。細胞劃痕和細胞小室(Transwell)試驗分析比較STC2基因沉默後對口腔鱗癌細胞侵襲和遷移的差異。最後用蛋白[質]印跡法(Western blot)檢測凋亡、轉移相關蛋白。結果：成功構建KB細胞STC2敲除細胞繫，CCK8增殖實驗結果顯示，STC2沉默後，細胞增殖被明顯抑製，其生長速度低于對照組(P<0.001)。在順鉑誘導細胞凋亡過程中，STC2沉默後KB細胞凋亡一定程度被促進。與KB細胞相比，STC2沉默後的細胞遷移和侵襲性明顯減弱。Western blot檢測髮現，沉默STC2後Bcl-2、促細胞遷移和侵襲蛋白Caveolin-1和β-catenin錶達下調，bax錶達上升。結論：STC2可能參與調控口腔鱗癌細胞KB的增殖凋亡，促進KB細胞的侵襲轉移能力，同時一定程度上減弱KB對化療藥物順鉑的敏感性。
배경여목적：구강합면부악성종류중약80%이상위구강린상세포암(oral squamous cell carcino-ma，OSCC)，수과거적궤십년진단급치료기술불단개진，기존활솔각몰유득도명현개선，5년생존솔잉소우50%。해연구지재탐토사개소2(stanniocalcin 2，STC2)대구강린암세포KB적증식、조망급침습천이적영향。방법：구건STC2적RNA간우재체，장기전염KB세포사STC2기인침묵후，채용CCK8실험검측STC2대KB세포증식적영향，통과APC Annexin V/7-AAD염색、류식세포술검측세포조망정황，분석STC2대세포조망적영향。세포화흔화세포소실(Transwell)시험분석비교STC2기인침묵후대구강린암세포침습화천이적차이。최후용단백[질]인적법(Western blot)검측조망、전이상관단백。결과：성공구건KB세포STC2고제세포계，CCK8증식실험결과현시，STC2침묵후，세포증식피명현억제，기생장속도저우대조조(P<0.001)。재순박유도세포조망과정중，STC2침묵후KB세포조망일정정도피촉진。여KB세포상비，STC2침묵후적세포천이화침습성명현감약。Western blot검측발현，침묵STC2후Bcl-2、촉세포천이화침습단백Caveolin-1화β-catenin표체하조，bax표체상승。결론：STC2가능삼여조공구강린암세포KB적증식조망，촉진KB세포적침습전이능력，동시일정정도상감약KB대화료약물순박적민감성。
Background and purpose:About 80%patients with oral and maxillofacial malignant tumor are oral squamous cell carcinoma (OSCC). OSCC is one of the most common cancers in the world with less than 50%survival rate over 5 years. This experiment aimed to explore the effect of stanniocalcin 2 (STC2) on apoptosis, proliferation, migration and invasion of OSCC cell. Methods:RNA interference (RNAi) vector pLKO.1-shSTC2 was constructed and transfected into KB cells. Cell proliferation and cell apoptosis were then assessed by CCK8, APC Annexin V/7-AAD and lfow cytometry. Differences of migration and invasion between KB scr and KB STC2i were examined by cell scratch and transwell tests. Finally, this study detected the apoptosis-associated proteins and metastasis-associated proteins by Western blot. Results:STC2 down-regulation plasmid was constructed and transfected into KB cells. CCK8 prolifera-tion assay revealed that the STC2 down-regulation inhibited KB cells proliferation. By treating with cisplatin, this study found that STC2 silence could facilitate cell apoptosis signiifcantly. With the knock down of STC2 gene, the expressions of Bcl-2, Caveolin-1 andβ-catenin were decreased but the expression of bax was obviously increased. Conclusion:These data suggest that STC2 may be involved in the apoptosis, proliferation, migration and invasion of OSCC KB cells. Simultaneously, it can signiifcantly weaken the sensitivity of KB cells to chemotherapeutic drug cisplatin.
