CAJ | 학술논문

目的：探讨胎盘组织中白细胞介素37（IL-37）的表达及其与重度子痫前期患者发病的关系。方法选取2012年11月至2013年11月在山东大学齐鲁医院住院分娩的重度子痫前期孕妇30例为重度子痫前期组，同时选取正常妊娠孕妇31例作为健康对照组。免疫组化SP法检测两组胎盘组织中IL-37蛋白的定位表达，蛋白印迹法检测两组胎盘组织中IL-37蛋白的表达水平，逆转录（RT）-PCR技术检测两组胎盘组织中IL-37 mRNA的表达水平，对分娩孕周和体质指数（BMI）与胎盘组织中IL-37 mRNA表达的相关性进行分析。结果（1）重度子痫前期组和健康对照组孕妇胎盘组织中均有IL-37蛋白的表达，表达部位均主要集中在胎盘合体滋养细胞的胞质内，少量则表达在细胞滋养细胞的胞质内。（2）重度子痫前期组和健康对照组孕妇胎盘组织中IL-37蛋白的表达水平分别为0.59±0.39及0.88±0.22,两组比较，差异有统计学意义（P<0.05）。重度子痫前期组和健康对照组胎盘组织中IL-37 mRNA的表达水平分别为0.55±0.17及1.11±0.21，两组比较，差异有统计学意义（P<0.05）。（3）重度子痫前期组孕妇分娩孕周与胎盘组织中IL-37 mRNA表达无相关性（r=0.209，P>0.05），健康对照组孕妇分娩孕周与胎盘组织中IL-37 mRNA表达也无相关性（r=-0.053，P>0.05）；重度子痫前期组孕妇BMI与胎盘组织中IL-37 mRNA表达无相关性（r=0.102，P>0.05），健康对照组孕妇BMI与胎盘组织中IL-37mRNA表达也无相关性（r=-0.115，P>0.05）。结论 IL-37在重度子痫前期孕妇胎盘组织中表达水平明显降低，可能对重度子痫前期的发病过程起到一定的保护性作用。
목적：탐토태반조직중백세포개소37（IL-37）적표체급기여중도자간전기환자발병적관계。방법선취2012년11월지2013년11월재산동대학제로의원주원분면적중도자간전기잉부30례위중도자간전기조，동시선취정상임신잉부31례작위건강대조조。면역조화SP법검측량조태반조직중IL-37단백적정위표체，단백인적법검측량조태반조직중IL-37단백적표체수평，역전록（RT）-PCR기술검측량조태반조직중IL-37 mRNA적표체수평，대분면잉주화체질지수（BMI）여태반조직중IL-37 mRNA표체적상관성진행분석。결과（1）중도자간전기조화건강대조조잉부태반조직중균유IL-37단백적표체，표체부위균주요집중재태반합체자양세포적포질내，소량칙표체재세포자양세포적포질내。（2）중도자간전기조화건강대조조잉부태반조직중IL-37단백적표체수평분별위0.59±0.39급0.88±0.22,량조비교，차이유통계학의의（P<0.05）。중도자간전기조화건강대조조태반조직중IL-37 mRNA적표체수평분별위0.55±0.17급1.11±0.21，량조비교，차이유통계학의의（P<0.05）。（3）중도자간전기조잉부분면잉주여태반조직중IL-37 mRNA표체무상관성（r=0.209，P>0.05），건강대조조잉부분면잉주여태반조직중IL-37 mRNA표체야무상관성（r=-0.053，P>0.05）；중도자간전기조잉부BMI여태반조직중IL-37 mRNA표체무상관성（r=0.102，P>0.05），건강대조조잉부BMI여태반조직중IL-37mRNA표체야무상관성（r=-0.115，P>0.05）。결론 IL-37재중도자간전기잉부태반조직중표체수평명현강저，가능대중도자간전기적발병과정기도일정적보호성작용。
Objective To investigate the expression of interleukin-37 (IL-37) in placenta tissue and its relationship with the pathogenesis of severe preeclampsia. Methods All the patients were recruited in Qilu Hospital of Shangdong University from November 2012 to November 2013. Among them, thirty patients with severe preeclampsia were assigned to the preeclampsia group, and thirty-one healthy pregnant women were assigned to the control group. Immunohistochemistry of SP was used to detect the IL-37 protein expression in placenta tissue of the two groups. The expression level of IL-37 in placenta tissue of the two groups was detected by western blot. Besides, reverse transcription(RT)-PCR was used to detect the expression of IL-37 mRNA. The correlation between the expression of IL-37 mRNA and the delivery gestational age, body mass index(BMI)was analyzed. Results (1) IL-37 were detected in the placenta of both the preeclampsia group and the control group, and the expression site mainly located in the syncytiotrophoblast, with a small amount in cytotrophoblast.(2)The expression levels of IL-37 protein in the preeclampsia group and the control group were 0.59 ± 0.39 and 0.88 ± 0.22, respectively. The IL-37 mRNA levels in the preeclampsia group and the control group were 0.55 ± 0.17 and 1.11 ± 0.21, respectively. Both decreased significantly when compared to the control group (P<0.05). (3) Significant correlation between the expression of IL-37 mRNA and the delivery gestational weeks(r=0.209, P>0.05) was seen neither in the preeclampsia group nor in the control group(r=-0.053, P>0.05). In the severe preeclampsia group, the pregnant women′s BMI had no significant correlation with IL-37 mRNA expression of placenta tissue(r=0.102, P>0.05), neither did the control group(r=-0.115, P>0.05). Conclusion IL-37 expression is significantly lower in severe preeclampsia placenta tissue than that in the normal pregnant women, which may play a protective role in the course of severe preeclampsia.