CAJ | 학술논문

目的：研究电针是否是通过影响LRP1 mRNA水平、提高LRP1的表达，从而促进脑内Aβ清除。方法：将4月龄APP／PS1转基因鼠，随机分为模型组、电针治疗组，以同窝同背景转基因阴性小鼠为正常对照组。电针干预“涌泉”、“百会”0．1 mA，15 min／次，隔日1次，治疗6周。治疗后，以免疫组化法观察脑组织LRP1阳性表达，以Western blotting法检测海马LRP1表达，以Real－time PCR法检测海马LRP1mRNA表达。结果：LRP1表达于脑微血管内皮细胞、胶质细胞、神经元等处。模型组海马LRP1蛋白、LRP1 mRNA相对表达量低于正常对照组（ P＜0．05），电针治疗组海马LRP1及LRP1 mRNA比模型组有上升趋势，但差异无统计学意义（ P＞0．05）。结论：电针干预可能影响5月龄APP／PS1转基因小鼠海马内所有细胞LRP1及LRP1 mRNA表达，可能是电针干预AD发病的潜在靶点，但实验方案还需进一步完善。
목적：연구전침시부시통과영향LRP1 mRNA수평、제고LRP1적표체，종이촉진뇌내Aβ청제。방법：장4월령APP／PS1전기인서，수궤분위모형조、전침치료조，이동와동배경전기인음성소서위정상대조조。전침간예“용천”、“백회”0．1 mA，15 min／차，격일1차，치료6주。치료후，이면역조화법관찰뇌조직LRP1양성표체，이Western blotting법검측해마LRP1표체，이Real－time PCR법검측해마LRP1mRNA표체。결과：LRP1표체우뇌미혈관내피세포、효질세포、신경원등처。모형조해마LRP1단백、LRP1 mRNA상대표체량저우정상대조조（ P＜0．05），전침치료조해마LRP1급LRP1 mRNA비모형조유상승추세，단차이무통계학의의（ P＞0．05）。결론：전침간예가능영향5월령APP／PS1전기인소서해마내소유세포LRP1급LRP1 mRNA표체，가능시전침간예AD발병적잠재파점，단실험방안환수진일보완선。
Objective:To observe whether LRP1 mRNA could be affected by electroacupuncture to improve the expression of LRP1 and strengthen the Aβclearance.Methods:4-month-old Alzheimer's disease models of APP/PS1 transgenic mice were randomly divided into a model group(M)and an electro-acupuncture group (EA).Non-transgenic litter-mate mice were used as a normal control group(C).Mice in EA group were given acupuncture at “Yongquan”,“Baihui” 0.1 mA, 15 min per time, 1 time every other day,for 6 weeks. After the treatment,immunohistochemistral method was used to observe the LRP1 expression.Western blotting was used to detect LRP1.Real-time PCR was used to detect LRP1mRNA expression in hippocampus.Results:LRP1 immunostaining was positive in microvascular endothelial cells, neurons, glial cells etc.In the model group, LRP1 protein, LRP1mRNA relative expression quantity were lower than those in the normal control group (P<0.05).LRP1 and LRP1mRNA in the EA group and the model group had an upward trend, but there was no significant difference ( P >0.05 ) .Conclusion:Electroacupuncture may affect all kinds of cells LRP1 and LRP1 mRNA expression in hippocampus of 5-month-old APP/PS1 mice models with Alzheimer's disease.It is probable that LRP1 may be a potential target of electroacupuncture intervention on AD.