中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2015年
5期
305-309
,共5页
陈诗赟%朴月善%付永娟%李卓%刘翠翠%卢德宏
陳詩赟%樸月善%付永娟%李卓%劉翠翠%盧德宏
진시빈%박월선%부영연%리탁%류취취%로덕굉
皮质发育畸形%自噬%癫痫
皮質髮育畸形%自噬%癲癇
피질발육기형%자서%전간
Malformations of cortical development%Autophagy%Epilepsy
目的:检测自噬相关蛋白Beclin-1、LC3及p62在难治性癫痫相关皮质发育畸形病变中的表达情况,初步探讨皮质发育畸形出现细胞水平异常的可能机制。方法选用18例手术切除的致痫灶标本,包括结节硬化综合征( TSC)、局灶性皮质发育不良( FCD)Ⅱb型及FCDⅠ型各6例。采用免疫组织化学染色( EnVison二步法)对3种蛋白分别在3组病变中细胞水平的表达情况进行定性分析,并计数Beclin-1、LC3染色阳性细胞数进行比较;应用Western blot方法对LC3蛋白在3组病变中的表达进行定量比较分析。结果免疫组织化学染色结果显示3种蛋白均主要表达于FCDⅡb型及TSC组中的形态异常神经元和气球样细胞/巨大细胞内。 Beclin-1为胞质内颗粒状或弥漫阳性,另可见到轴索强阳性表达;LC3为胞质内弥漫阳性或核周阳性;p62为胞质内弥漫阳性,部分为核及核周阳性。此外,形态异常神经元中Beclin-1、LC3及p62阳性程度高于气球样细胞/巨大细胞。而作为对照的FCDⅠ组中仅有个别或部分细胞呈弱阳性表达。 Beclin-1和LC3染色中阳性细胞数均为TSC组>FCDⅡb组>FCDⅠ组,且TSC组、FCDⅡb组与FCDⅠ组的差异均具有统计学意义( P<0.05)。Western blot结果显示LC3蛋白在病灶组织内的表达量为 TSC组<FCDⅡb组<FCDⅠ组。结论TSC、FCDⅡb中的形态异常神经元和气球样细胞/巨大细胞中存在自噬抑制,推测自噬的异常可能与蛋白质的异常堆积密切相关;但形态异常神经元和气球样细胞中自噬异常具体机制不完全一致。
目的:檢測自噬相關蛋白Beclin-1、LC3及p62在難治性癲癇相關皮質髮育畸形病變中的錶達情況,初步探討皮質髮育畸形齣現細胞水平異常的可能機製。方法選用18例手術切除的緻癇竈標本,包括結節硬化綜閤徵( TSC)、跼竈性皮質髮育不良( FCD)Ⅱb型及FCDⅠ型各6例。採用免疫組織化學染色( EnVison二步法)對3種蛋白分彆在3組病變中細胞水平的錶達情況進行定性分析,併計數Beclin-1、LC3染色暘性細胞數進行比較;應用Western blot方法對LC3蛋白在3組病變中的錶達進行定量比較分析。結果免疫組織化學染色結果顯示3種蛋白均主要錶達于FCDⅡb型及TSC組中的形態異常神經元和氣毬樣細胞/巨大細胞內。 Beclin-1為胞質內顆粒狀或瀰漫暘性,另可見到軸索彊暘性錶達;LC3為胞質內瀰漫暘性或覈週暘性;p62為胞質內瀰漫暘性,部分為覈及覈週暘性。此外,形態異常神經元中Beclin-1、LC3及p62暘性程度高于氣毬樣細胞/巨大細胞。而作為對照的FCDⅠ組中僅有箇彆或部分細胞呈弱暘性錶達。 Beclin-1和LC3染色中暘性細胞數均為TSC組>FCDⅡb組>FCDⅠ組,且TSC組、FCDⅡb組與FCDⅠ組的差異均具有統計學意義( P<0.05)。Western blot結果顯示LC3蛋白在病竈組織內的錶達量為 TSC組<FCDⅡb組<FCDⅠ組。結論TSC、FCDⅡb中的形態異常神經元和氣毬樣細胞/巨大細胞中存在自噬抑製,推測自噬的異常可能與蛋白質的異常堆積密切相關;但形態異常神經元和氣毬樣細胞中自噬異常具體機製不完全一緻。
목적:검측자서상관단백Beclin-1、LC3급p62재난치성전간상관피질발육기형병변중적표체정황,초보탐토피질발육기형출현세포수평이상적가능궤제。방법선용18례수술절제적치간조표본,포괄결절경화종합정( TSC)、국조성피질발육불량( FCD)Ⅱb형급FCDⅠ형각6례。채용면역조직화학염색( EnVison이보법)대3충단백분별재3조병변중세포수평적표체정황진행정성분석,병계수Beclin-1、LC3염색양성세포수진행비교;응용Western blot방법대LC3단백재3조병변중적표체진행정량비교분석。결과면역조직화학염색결과현시3충단백균주요표체우FCDⅡb형급TSC조중적형태이상신경원화기구양세포/거대세포내。 Beclin-1위포질내과립상혹미만양성,령가견도축색강양성표체;LC3위포질내미만양성혹핵주양성;p62위포질내미만양성,부분위핵급핵주양성。차외,형태이상신경원중Beclin-1、LC3급p62양성정도고우기구양세포/거대세포。이작위대조적FCDⅠ조중부유개별혹부분세포정약양성표체。 Beclin-1화LC3염색중양성세포수균위TSC조>FCDⅡb조>FCDⅠ조,차TSC조、FCDⅡb조여FCDⅠ조적차이균구유통계학의의( P<0.05)。Western blot결과현시LC3단백재병조조직내적표체량위 TSC조<FCDⅡb조<FCDⅠ조。결론TSC、FCDⅡb중적형태이상신경원화기구양세포/거대세포중존재자서억제,추측자서적이상가능여단백질적이상퇴적밀절상관;단형태이상신경원화기구양세포중자서이상구체궤제불완전일치。
Objective To study the expression of autophagy-related proteins ( Beclin-1, LC3 and p62) in brain tissue with malformations of cortical development and related molecular pathogenesis. Methods The brain tissue of 18 cases with epileptogenic foci resection, including 6 cases of tuberous sclerosis complex (TSC), 6 cases of focal cortical dysplasia type Ⅱb (FCD Ⅱb) and 6 cases of focal cortical dysplasia type Ⅰ ( FCD Ⅰ) , were retrieved.Immunohistochemical study for Beclin-1, LC3 and p62 proteins was performed.The degree of positivity for Beclin-1 and LC3 proteins was compared.Western blot was used to quantitatively analyze the LC3 protein in focal lesion of each disease groups.Results Immunohistochemical study showed that the three proteins were mainly expressed in the dysmorphic neurons and balloon cells/giant cells of TSC and FCD Ⅱb.The positivity was more intense in the dysmorphic neurons than the other cell types.Immunostaining for Beclin-1 showed granular or diffuse cytoplasmic positivity, in addition to the strong expression in axons.On the other hand, LC3 showed diffuse or perinuclear cytoplasmic expression.The staining for p62 was mainly cytoplasmic or perinuclear and sometimes nuclear.In FCD typeⅠ, only individual cells showed positive expression for the three proteins.The number of Beclin-1 and LC3-positive cells was larger in TSC group, followed by FCDⅡb group and FCDⅠgroup.And there were significant differences between TSC group and FCDⅠgroup, as well as FCDⅡb group and FCDⅠgroup (P<0.05).Quantitative expression of LC3 protein by Western blot showed smaller amount in TSC group, followed by FCD Ⅱb group and FCDⅠ group.Conclusions The dysmorphic neurons and balloon cells/giant cells of TSC and FCD Ⅱb show abnormality in autophagy, resulting in intracytoplasmic protein accumulation.There are differences in molecular pathogenesis in these cell types.