陕西医学杂志
陝西醫學雜誌
협서의학잡지
SHAANXI MEDICAL JOURNAL
2015年
6期
656-659
,共4页
曹艳%何利红%孙赟%于泳
曹豔%何利紅%孫赟%于泳
조염%하리홍%손빈%우영
黄芪甲苷%胃肿瘤%肿瘤转移%基质金属蛋白酶%细胞外信号调节激酶
黃芪甲苷%胃腫瘤%腫瘤轉移%基質金屬蛋白酶%細胞外信號調節激酶
황기갑감%위종류%종류전이%기질금속단백매%세포외신호조절격매
Astragaloside IV%Gastric neoplasms%Neoplasm metastasis%Matrix metalloproteinases ERK
目的：研究黄芪甲苷对胃癌细胞SGC7901侵袭能力的影响并探讨其可能的机制。方法：用终浓度为100μg ／m l黄芪甲苷处理实验组细胞48 h ，之后采用流式细胞仪检测对照组和实验组细胞凋亡情况，T ransw ell细胞侵袭实验检测黄芪甲苷对细胞侵袭能力的影响，并采用PCR和Western‐blot方法检测黄芪甲苷对细胞MMP‐2和MMP‐9表达的影响，最后采用Western‐blot方法检测黄芪甲苷处理对细胞ERK通路的活化情况的影响。结果：100μg／ml黄芪甲苷处理胃癌细胞S G C790148 h后，细胞凋亡比例未发生明显变化，细胞侵袭能力显著降低，M M P‐2和M M P‐9分子的m RN A和蛋白水平均下降，此外，细胞内的磷酸化ERK蛋白水平在黄芪甲苷处理后表达减少。结论：在体外黄芪甲苷能够通过减少细胞MMP‐2和MMP‐9的表达而发挥抑制胃癌细胞侵袭的能力，而且其抑侵袭作用一定程度上是通过抑制细胞外信号调节激酶（ERK ）信号通路实现的。
目的：研究黃芪甲苷對胃癌細胞SGC7901侵襲能力的影響併探討其可能的機製。方法：用終濃度為100μg ／m l黃芪甲苷處理實驗組細胞48 h ，之後採用流式細胞儀檢測對照組和實驗組細胞凋亡情況，T ransw ell細胞侵襲實驗檢測黃芪甲苷對細胞侵襲能力的影響，併採用PCR和Western‐blot方法檢測黃芪甲苷對細胞MMP‐2和MMP‐9錶達的影響，最後採用Western‐blot方法檢測黃芪甲苷處理對細胞ERK通路的活化情況的影響。結果：100μg／ml黃芪甲苷處理胃癌細胞S G C790148 h後，細胞凋亡比例未髮生明顯變化，細胞侵襲能力顯著降低，M M P‐2和M M P‐9分子的m RN A和蛋白水平均下降，此外，細胞內的燐痠化ERK蛋白水平在黃芪甲苷處理後錶達減少。結論：在體外黃芪甲苷能夠通過減少細胞MMP‐2和MMP‐9的錶達而髮揮抑製胃癌細胞侵襲的能力，而且其抑侵襲作用一定程度上是通過抑製細胞外信號調節激酶（ERK ）信號通路實現的。
목적：연구황기갑감대위암세포SGC7901침습능력적영향병탐토기가능적궤제。방법：용종농도위100μg ／m l황기갑감처리실험조세포48 h ，지후채용류식세포의검측대조조화실험조세포조망정황，T ransw ell세포침습실험검측황기갑감대세포침습능력적영향，병채용PCR화Western‐blot방법검측황기갑감대세포MMP‐2화MMP‐9표체적영향，최후채용Western‐blot방법검측황기갑감처리대세포ERK통로적활화정황적영향。결과：100μg／ml황기갑감처리위암세포S G C790148 h후，세포조망비례미발생명현변화，세포침습능력현저강저，M M P‐2화M M P‐9분자적m RN A화단백수평균하강，차외，세포내적린산화ERK단백수평재황기갑감처리후표체감소。결론：재체외황기갑감능구통과감소세포MMP‐2화MMP‐9적표체이발휘억제위암세포침습적능력，이차기억침습작용일정정도상시통과억제세포외신호조절격매（ERK ）신호통로실현적。
Objective:To investigate the effects of astragaloside IV on the invasive capacity of gastric canc‐er cell line SGC7901 and to explore the potential mechanisms involved .Methods:The SGC7901 cell was treated with or without 100μg/ml astragaloside IV for 48h .After astragaloside IV administration ,the apoptotic cells between the control group and the experimental group were detected by flow cytometry ,the invasive capacity was measured by tr‐answell invasion assay ,the expression of MMP‐2 and MMP‐9 was leveled by PCR and Western‐blot .Finally ,the status of the ERK pathway was analyzed by Western‐blot to further elucidate the impact of astragaloside IV on SGC7901 cells .Results :After being treated with or without 100μg/ml astragaloside IV for 48h ,the SGC7901 cell possessed non‐significant proportion of apoptosis .The invasive capacity ,the MMP‐2 and the MMP‐9 expression were obviously impaired after astragaloside IV treatment .Besides ,Western‐blot assay showed that the phosphorylation of ERK was decreased in the experimental group .Conclusion:Astragaloside IV can inhibit the invasive capacity of gas‐tric cancer cells through diminishing the MMP‐2 and the MMP‐9 expression .And the anti‐invasion effects of astra‐galoside IV is possibly mediated by inactivating the ERK pathway .
