CAJ | 학술논문

目的：检测白细胞介素-23（IL-23）在舌鳞状细胞癌组织中表达水平与临床预后的关系，研究舌鳞状细胞癌细胞系SCC9经IL-23处理后抗凋亡及耐药能力的变化。方法免疫组织化学法检测28例舌鳞状细胞癌患者组织中IL-23的表达情况；实时定量聚合酶链反应（qRT-PCR）检测不同浓度的IL-23处理后，SCC9中无翅基因相关整合位点（Wnt）1及c-myc的mRNA表达水平；结合小分子干扰RNA（siRNA），Western?blot法检测IL-23对SCC9细胞的β-连环蛋白（β-catenin）和B淋巴细胞瘤-2（Bcl-2）、三磷酸腺苷结合转运蛋白G超家族成员2（ABCG2）、P-糖蛋白（P-gp）表达影响及潜在机制；甲基噻唑基四唑法检测SCC9细胞在IL-23作用下对顺铂的抗凋亡能力改变。结果舌鳞状细胞癌组织中IL-23表达强度与淋巴结转移、神经侵犯及治疗复发有相关性（P<0.05）；?IL-23可增强癌细胞抗凋亡蛋白Bcl-2和耐药相关蛋白ABCG2、P-gp的表达，并且与Wnt通路活化程度密切相关；IL-23能够显著加强SCC9细胞对顺铂的抵抗性（P<0.01）。结论 IL-23通过上调舌鳞状细胞癌细胞的Wnt通路增强其抗凋亡及耐药能力。
목적：검측백세포개소-23（IL-23）재설린상세포암조직중표체수평여림상예후적관계，연구설린상세포암세포계SCC9경IL-23처리후항조망급내약능력적변화。방법면역조직화학법검측28례설린상세포암환자조직중IL-23적표체정황；실시정량취합매련반응（qRT-PCR）검측불동농도적IL-23처리후，SCC9중무시기인상관정합위점（Wnt）1급c-myc적mRNA표체수평；결합소분자간우RNA（siRNA），Western?blot법검측IL-23대SCC9세포적β-련배단백（β-catenin）화B림파세포류-2（Bcl-2）、삼린산선감결합전운단백G초가족성원2（ABCG2）、P-당단백（P-gp）표체영향급잠재궤제；갑기새서기사서법검측SCC9세포재IL-23작용하대순박적항조망능력개변。결과설린상세포암조직중IL-23표체강도여림파결전이、신경침범급치료복발유상관성（P<0.05）；?IL-23가증강암세포항조망단백Bcl-2화내약상관단백ABCG2、P-gp적표체，병차여Wnt통로활화정도밀절상관；IL-23능구현저가강SCC9세포대순박적저항성（P<0.01）。결론 IL-23통과상조설린상세포암세포적Wnt통로증강기항조망급내약능력。
ObjectiveThis?study?aims?to?detect?the?expression?level?of?interleukin-23?(IL-23)?in?tongue?squamous?cell?carcinoma?tissues?and?its?relationship?with?clinical?prognosis,?as?well?as?explore?the?anti-apoptotic?and?drug?resistance?of?the?tongue?squamous?cell?line-SCC9?before?and?after?treatment?with?IL-23.?Methods???The?expression?of?IL-23?in?tumor?tissues?from?28?tongue?cancer?patients?was?analyzed?by?immunohistochemistry?assay.?Quantitative?real-time?polymerase?chain?reac-tion?(qRT-PCR)?was?used?to?detect?the?mRNA?expression?of?Wingless-related?integration?site?(Wnt)1?and?c-myc?in?SCC9?cells?treated with different IL-23 concentrations. After interferencing the β-catenin with small interfering RNA (siRNA), the expres-sion of β-catenin, B-cell lymphoma-2 (Bcl-2), ATP-binding cassette sub-family G member 2 (ABCG2), and permeability-glycoprotein?(P-gp)?in?SCC9?was?measured?by?Western?blot?analysis.?The?effect?of?IL-23?on?the?apoptotic?resistance?of?SCC9?to?cisplatin?was?examined?by?methyl?thiazolyl?tetrazolium?test.?Results???The?expression?of?IL-23?in?tongue?cancer?tissues?was?correlated?with?lymphatic?metastasis,?nerve?invasion,?and?the?recurrence?after?therapy?(P<0.05).?After?dealing?with?IL-23,?SCC9?showed?the?upregulation?effect?of?Bcl-2,?ABCG2?and?P-gp?expressions.?IL-23?was?closely?related?to?the?activation?level?of?the?Wnt?pathway?and?significantly?strengthened?the?resistance?to?cisplatin?(P<0.01).?Conclusion???IL-23?activates?the?Wnt?pathway?in?tongue?squamous?cell?carcinoma,?thereby?enhancing?its?resistance?to?apoptosis?and?drug.