CAJ | 학술논문

目的：检测白细胞介素-21（IL-21）和破骨细胞核因子κB受体活化因子配体（RANKL）在根尖囊肿和根尖肉芽肿中的表达，分析两者在根尖周病中的关系，探讨IL-21在根尖周炎发病机制中的作用。方法收集根尖囊肿23例和根尖肉芽肿32例作为实验组，记录相关病例的病损大小及有无叩痛表现；10例健康牙龈组织为对照组。利用免疫组织化学法检测所有样本中IL-21和RANKL蛋白的表达水平，分析IL-21的表达水平与RANKL表达、根尖病灶大小及叩痛的相关性。结果所有病变组织均可检测到IL-21阳性细胞，而健康牙龈组织则未检测到IL-21的表达。根尖囊肿和肉芽肿中IL-21的表达强度分别为59.92±6.57和36.80±6.81，RANKL的表达强度分别为68.81±18.59和36.12±14.87。根尖囊肿组两种蛋白的表达水平均高于肉芽肿组（P<0.05）。相关性分析表明，IL-21的表达水平与RANKL及根尖病灶大小均呈正相关关系（P<0.05）。结论 IL-21存在于人慢性根尖周炎病损组织中，其表达水平与RANKL的表达量及病损大小呈正相关关系；IL-21可能通过促进RANKL蛋白的表达参与慢性根尖周炎的发病机制。
목적：검측백세포개소-21（IL-21）화파골세포핵인자κB수체활화인자배체（RANKL）재근첨낭종화근첨육아종중적표체，분석량자재근첨주병중적관계，탐토IL-21재근첨주염발병궤제중적작용。방법수집근첨낭종23례화근첨육아종32례작위실험조，기록상관병례적병손대소급유무고통표현；10례건강아간조직위대조조。이용면역조직화학법검측소유양본중IL-21화RANKL단백적표체수평，분석IL-21적표체수평여RANKL표체、근첨병조대소급고통적상관성。결과소유병변조직균가검측도IL-21양성세포，이건강아간조직칙미검측도IL-21적표체。근첨낭종화육아종중IL-21적표체강도분별위59.92±6.57화36.80±6.81，RANKL적표체강도분별위68.81±18.59화36.12±14.87。근첨낭종조량충단백적표체수평균고우육아종조（P<0.05）。상관성분석표명，IL-21적표체수평여RANKL급근첨병조대소균정정상관관계（P<0.05）。결론 IL-21존재우인만성근첨주염병손조직중，기표체수평여RANKL적표체량급병손대소정정상관관계；IL-21가능통과촉진RANKL단백적표체삼여만성근첨주염적발병궤제。
ObjectiveThis?study?aimed?to?detect?the?immunoexpression?of?interleukin-21?(IL-21)?and?receptor?activator?of?nuclear?factor κB ligand (RANKL) in periapical granulomas (PGs) and radicular cysts (RCs). The interaction of IL-21 with RANKL?and?its?role?in?periapical?pathogenesis?were?also?speculated.?Methods A total of 32 PGs and 23 RCs were selected as?experimental?samples.?Lesion?size?and?occurrence?of?tenderness?were?recorded.?Up?to?10?healthy?gingival?tissues?were?collected?as?normal?control?samples.?All?tissues?were?subjected?to?immunohistochemical?analysis?with?anti-human?IL-21?and?RANKL?polyclonal?antibodies.?The?correlations?of?IL-21?with?RANKL,?lesion?size,?and?the?occurrence?of?tenderness?of?the?PGs and RCs were evaluated.Results???IL-21-positive?cells?were?detected?in?all?periapical?lesion?tissues?but?not?in?normal?tissues.?In?the?cyst?group?and?granuloma?group,?the?corresponding?expression?levels?of?IL-21?were?59.92±6.57?and?36.80± 6.81,?whereas?those?of?RANKL?were?68.81±18.59?and?36.12±14.87,?respectively.?Moreover,?t-test?revealed?a?significantly?higher expression of IL-21 and RANKL in RCs than in PGs (P<0.05).?IL-21?and?RANKL?were?positively?correlated?in?both?PGs and RCs (P<0.05).?Furthermore,?IL-21?was?correlated?with?lesion?size?(P<0.05).?Conclusion???This?study?demonstrated?that?IL-21?is?potentially?involved?in?the?pathogenesis?of?apical?periodontitis?lesions.?A?role?in?the?exacerbation?of?chronic?inflammation,?as?well?as?in?bone?resorption,?is?suspected.?Further?studies?are?required?to?elucidate?the?specific?functions?of?IL-21?in?periradicular?inflammatory?processes.