CAJ | 학술논문

目的：探讨创伤愈合过程中神经生长因子（NGF）调控降钙素基因相关肽（CGRP）的表达，以及影响MG-63细胞增殖的作用机制。方法加入不同质量浓度的NGF刺激MG-63细胞，1、2、3、4?d后收集样本，采用酶联免疫吸附测定法（ELISA）检测CGRP的表达，实时荧光定量聚合酶链反应（RT-QPCR）检测CGRP?mRNA的表达，采用细胞计数法（CCK-8）检测MG-63细胞的增殖；在MG-63细胞中加入NGF受体阻断剂，采用RT-QPCR和CCK-8检测CGRP?mRNA的表达及MG-63细胞的增殖效率。结果在NGF作用下，MG-63细胞分泌的CGRP表达量明显上调，随NGF质量浓度升高，CGRP表达量也相应升高，具有浓度依赖性，CGRP表达量随NGF作用时间延长也相应增加（P<0.05）；随NGF质量浓度升高和作用时间延长，MG-63细胞的增殖效率也相应增加（P<0.05）。加入NGF受体阻断剂后此作用相应减弱（P<0.05）。结论在创伤愈合过程中NGF能通过上调CGRP的表达量影响MG-63细胞的增殖，从而促进创伤愈合。
목적：탐토창상유합과정중신경생장인자（NGF）조공강개소기인상관태（CGRP）적표체，이급영향MG-63세포증식적작용궤제。방법가입불동질량농도적NGF자격MG-63세포，1、2、3、4?d후수집양본，채용매련면역흡부측정법（ELISA）검측CGRP적표체，실시형광정량취합매련반응（RT-QPCR）검측CGRP?mRNA적표체，채용세포계수법（CCK-8）검측MG-63세포적증식；재MG-63세포중가입NGF수체조단제，채용RT-QPCR화CCK-8검측CGRP?mRNA적표체급MG-63세포적증식효솔。결과재NGF작용하，MG-63세포분비적CGRP표체량명현상조，수NGF질량농도승고，CGRP표체량야상응승고，구유농도의뢰성，CGRP표체량수NGF작용시간연장야상응증가（P<0.05）；수NGF질량농도승고화작용시간연장，MG-63세포적증식효솔야상응증가（P<0.05）。가입NGF수체조단제후차작용상응감약（P<0.05）。결론재창상유합과정중NGF능통과상조CGRP적표체량영향MG-63세포적증식，종이촉진창상유합。
ObjectiveTo?investigate?the?nerve?growth?factor?(NGF)?regulating?the?expression?of?calcitonin?gene-related?peptide?(CGRP)?in?promoting?the?proliferation?of?osteoblast-like?cell?(MG-63)?and?thus?illustrate?the?mechanism?of?the?NGF?in?wound?healing.?Methods???Different?concentrations?of?NGF?were?used?to?stimulate?MG-63.?The?expression?of?CGRP?was?detected?by?real-time?quantitative?polymerase?chain?reaction?(RT-QPCR)?and?enzyme-linked?immunosorbent?assay?after?1,?2,?3,?and?4?days.?The?proliferation?of?MG-63?was?detected?by?cell?counting?kit-8?(CCK-8).?The?expression?of?CGRP?mRNA?and?the?proliferation?of?MG-63?were?then?detected?by?RT-QPCR?and?CCK-8?after?adding?the?NGF?receptor?blocker.?Results???Com-pared?with?the?blank?control?group,?the?expression?of?CGRP?significantly?increased?by?stimulating?the?NGF.?The?expression?of?CGRP?was?positively?related?to?the?concentration?of?NGF?(P<0.05).?Moreover,?the?expression?of?CGRP?increased?by?pro-longing?the?NGF?stimulation?time.?The?proliferation?of?MG-63?increased?after?stimulating?the?NGF?(P<0.05).?After?adding?the?NGF?receptor?blocker,?the?expression?of?CGRP?and?the?proliferation?of?MG-63?correspondingly?decreased?(P<0.05).?Conclusion???NGF?can?up-regulate?the?expression?of?CGRP?and?increase?the?proliferation?of?MG-63.?Therefore,?NGF?plays?a?significant?role?in?wound?healing.