CAJ | 학술논문

目的：探讨干扰素诱导跨膜蛋白3（ IFITM3）在结肠癌组织中的表达及其临床意义。方法采用实时定量PCR和免疫组化法检测正常结肠组织、结肠腺瘤和结肠腺癌组织中IFITM3 mRNA和蛋白的表达水平，分析其与结肠癌患者临床病理特征及预后的关系。结果 IFITM3 mRNA在正常结肠组织、结肠腺瘤、未转移结肠腺癌和转移结肠腺癌组织中的表达水平分别为4．49±0．69、7．32±0．76、12．19±1．34和18．37±0．61，差异有统计学意义（ P＜0．001）。 IFITM3蛋白在正常结肠组织、结肠腺瘤、未转移结肠腺癌和转移结肠腺癌组织中的阳性表达率分别为3．9％（2／51）、11．3％（14／124）、19．0％（19／100）和69．0％（78／113）。 IFITM3蛋白表达与肿瘤周围组织浸润、肝转移、结肠旁淋巴结转移、结肠系膜淋巴结转移、系膜根部淋巴结转移、腹网膜转移和美国癌症联合委员会（ AJCC）分期有关（均P＜0．05）。 IFITM3蛋白阳性表达结肠癌患者的5年生存率为40．2％， IFITM3蛋白阴性表达结肠癌患者的5年生存率为88．8％，差异有统计学意义（ P＜0．001）。结论 IFITM3表达与结肠癌转移程度及预后有关。
목적：탐토간우소유도과막단백3（ IFITM3）재결장암조직중적표체급기림상의의。방법채용실시정량PCR화면역조화법검측정상결장조직、결장선류화결장선암조직중IFITM3 mRNA화단백적표체수평，분석기여결장암환자림상병리특정급예후적관계。결과 IFITM3 mRNA재정상결장조직、결장선류、미전이결장선암화전이결장선암조직중적표체수평분별위4．49±0．69、7．32±0．76、12．19±1．34화18．37±0．61，차이유통계학의의（ P＜0．001）。 IFITM3단백재정상결장조직、결장선류、미전이결장선암화전이결장선암조직중적양성표체솔분별위3．9％（2／51）、11．3％（14／124）、19．0％（19／100）화69．0％（78／113）。 IFITM3단백표체여종류주위조직침윤、간전이、결장방림파결전이、결장계막림파결전이、계막근부림파결전이、복망막전이화미국암증연합위원회（ AJCC）분기유관（균P＜0．05）。 IFITM3단백양성표체결장암환자적5년생존솔위40．2％， IFITM3단백음성표체결장암환자적5년생존솔위88．8％，차이유통계학의의（ P＜0．001）。결론 IFITM3표체여결장암전이정도급예후유관。
Objective To investigate the expression of IFITM3 in colorectal carcinoma and its clinical significance.Methods 213 patients with colon ademocarcinoma and 214 patients with colon adenoma treated by surgery in our hospital from March 2008 to June 2010 were included in this study.The levels of IFITM3 in normal colon nucosa, adenoma, and adenocarcinoma tissues were detected by real-time PCR and immunochemistry, and its relationship with metastasis and prognosis in 213 colorectal cancer patients was analyzed.Results The IFITM3 mRNA level in metastatic tumor group was 18.37 ±0.61, significantly higher than that in the normal 4.49±0.69 and non-metastases groups (7.32±0.76;F=460.380, P<0.001 ) .The positive rate of IFITM3 protein expression in metastatic tumor group ( 69.0%) was significantly higher than that in the normal (3.9%),non-metastasies groups (19.0%) and adenoma groups ( 11.3%) .Our clinical analysis confirmed that the IFITM3 expression was associated with peritumoral invasion, hepatic metastases, metastases of para-colonic lymph nodes, mesocolonic lymph nodes and mesenteric root lymph nodes , omental metastasis and AJCC classification ( P<0.05 ) .Furthermore, the survival curve analysis showed that patients with lower IFITM3 level expression had a higher 5-year survival rate (88.8%) than that in the patients with higher expression (40.2%,P<0.001).C onclusions IFITM3 expression has a positive correlation with metastasis and prognosis in patients with colorectal carcinoma.