CAJ | 학술논문

目的研究甘草悬浮培养细胞对芦荟大黄素的生物转化。 　　方法将芦荟大黄素与甘草细胞悬浮培养细胞共孵育7d后，培养液经由大孔吸附树脂处理，细胞用甲醇提取，合并两部分浸膏后依次采用 Sephadex LH-20凝胶柱色谱、正相硅胶柱色谱、反相半制备 HPLC 等技术进行分离纯化，利用质谱和核磁共振波谱技术进行结构鉴定。 　　结果分离得到了一个芦荟大黄素的选择性糖基化产物——芦荟大黄素-(3-羟甲基)-O-β-D-葡萄糖苷。 　　结论甘草悬浮培养细胞对芦荟大黄素具有位置选择性糖基化能力。
목적연구감초현부배양세포대호회대황소적생물전화。 　　방법장호회대황소여감초세포현부배양세포공부육7d후，배양액경유대공흡부수지처리，세포용갑순제취，합병량부분침고후의차채용 Sephadex LH-20응효주색보、정상규효주색보、반상반제비 HPLC 등기술진행분리순화，이용질보화핵자공진파보기술진행결구감정。 　　결과분리득도료일개호회대황소적선택성당기화산물——호회대황소-(3-간갑기)-O-β-D-포도당감。 　　결론감초현부배양세포대호회대황소구유위치선택성당기화능력。
Objective To investigate the biotransformation of aloe-emodin by cell suspension cultures of Glycyrrhiza uralensis. Methods Aloe-emodin was added into cell suspension cultures of Glycyrrhiza uralensis and incubated for another 7 d. To obtain residues, the culture medium was processed by a macroporous adsorbent resin column or the cells was extracted with MeOH, and then these two residues was separated and purified by Sephadex LH-20 column chromatography, silica gel column chromatography and reversed-phase semi-preparative HPLC, successively. Through the processes, a metabolite was achieved. The structure of the metabolite was elucidated on the basis of spectroscopic analyses. Results A regio-selectively glycosylated product, aloe-emodin-3-(hydroxymethyl)-O-β-D-glucoside, was isolated from the biotransformation of aloe-emodin by G. uralensis cell suspension cultures. Conclusion The result indicates that the G. uralensis cell suspension cultures possess the regio-selective glycosylated capacity for aloe-emodin.