CAJ | 학술논문

目的建立人脂肪组织中分离血管周围干细胞（PSCs）的方法，并研究其在脂肪组织细胞中所占的比例，为血管周围干细胞作为骨和软骨组织工程新的种子细胞奠定基础。 　　方法取人的脂肪组织分别用 I 型胶原酶和 II 型胶原酶消化得到血管基质成分（SVF），用细胞计数仪及流式细胞仪检测 SVF 中细胞密度、活细胞比例和 PSCs 细胞所占的比例。 　　结果用细胞计数仪分析得出用 II 型胶原酶消化脂肪组织所得到的 SVF 中活细胞比例更高，且差异具有统计学意义（P<0.01），而细胞密度有增高趋势（P=0.18）。用流式细胞仪检测得出使用 II 型胶原酶消化脂肪组织所得 SVF中 PSCs 比例更高，且差异具有统计学意义（P<0.01）。使用 I 型胶原酶消化每10 ml 脂肪组织中可以分离得到PSCs 活细胞为（7.98±2.38）×105个，使用 II 型胶原酶消化则每10 ml 脂肪组织中可以分离得到 PSCs 活细胞为（16.68±4.56）×105个。 　　结论使用 II 型胶原酶消化脂肪组织可以得到更多的血管周围干细胞 PSCs，其在脂肪组织中的含量可以满足骨和软骨损伤后自体细胞移植修复的需要。
목적건립인지방조직중분리혈관주위간세포（PSCs）적방법，병연구기재지방조직세포중소점적비례，위혈관주위간세포작위골화연골조직공정신적충자세포전정기출。 　　방법취인적지방조직분별용 I 형효원매화 II 형효원매소화득도혈관기질성분（SVF），용세포계수의급류식세포의검측 SVF 중세포밀도、활세포비례화 PSCs 세포소점적비례。 　　결과용세포계수의분석득출용 II 형효원매소화지방조직소득도적 SVF 중활세포비례경고，차차이구유통계학의의（P<0.01），이세포밀도유증고추세（P=0.18）。용류식세포의검측득출사용 II 형효원매소화지방조직소득 SVF중 PSCs 비례경고，차차이구유통계학의의（P<0.01）。사용 I 형효원매소화매10 ml 지방조직중가이분리득도PSCs 활세포위（7.98±2.38）×105개，사용 II 형효원매소화칙매10 ml 지방조직중가이분리득도 PSCs 활세포위（16.68±4.56）×105개。 　　결론사용 II 형효원매소화지방조직가이득도경다적혈관주위간세포 PSCs，기재지방조직중적함량가이만족골화연골손상후자체세포이식수복적수요。
Objective To establish the method of sorting out perivascular stem cells (PSCs) from human adipose tissue and study the proportion of these cells in adipose tissue cells. This research is to explore new seed cells for the bone and cartilage tissue engineering. Methods Stromal vascular fraction (SVF) was got from human adipose tissue that was digested by collagenase type I or collagenase type II. The cell density, proportion of living cells and proportion of PSCs in SVF were tested by the cell count and flow cytometry (FCM). Results The proportion of living cells in SVF digested by collagenase type II was much higher through analyzing by the cell count and the difference was statistically significant (P<0.01). The cellular density in SVF digested by collagenase type II tend to increase (P=0.18). The proportion of PSCs in SVF digested by collagenase type II was much higher through analyzing by the flow cytometry, and the difference was statistically significant (P<0.01). (7.98 ± 2.38) × 105 PSCs were got in 10 ml human adipose tissue digested by collagenase type I, while (16.68 ± 4.56) × 105 PSCs were got in 10 ml human adipose tissue digested by collagenase type II. Conclusion A higher amount of PSCs can be got from human adipose digested by collagenase type II, and the content of PSCs in the adipose tissue can satisfy the needs of autologous cell transplantation for the bone and cartilage repair.