CAJ | 학술논문

氨基化合物在植物生长发育中起着重要作用。本文建立了一种基于柱前衍生-超高效液相色谱-质谱联用技术的植物提取液中氨基化合物代谢谱的分析方法。以烟草鲜叶为例，共检测出87种氨基化合物。其中43种氨基化合物的定量分析结果表明，方法的线性相关系数在0.993~0.999之间，线性范围可达到4个数量级，检出限为0.03~6.58 ng／mL，日内、日间精密度分别为0.7％~15.6％、0.8％~22.9％，回收率为74.4％~122.7％。采用该方法考察了打顶对不同部位烟草鲜叶中氨基化合物代谢谱的影响，结果显示打顶处理对上部叶影响最大，打顶后上部叶氮代谢主要流向生物碱合成方向。该方法充分利用了三重四极杆串联质谱和高分辨串联质谱技术的优势，可实现植物提取液中氨基代谢物的高选择性、高灵敏度分析。
안기화합물재식물생장발육중기착중요작용。본문건립료일충기우주전연생-초고효액상색보-질보련용기술적식물제취액중안기화합물대사보적분석방법。이연초선협위례，공검측출87충안기화합물。기중43충안기화합물적정량분석결과표명，방법적선성상관계수재0.993~0.999지간，선성범위가체도4개수량급，검출한위0.03~6.58 ng／mL，일내、일간정밀도분별위0.7％~15.6％、0.8％~22.9％，회수솔위74.4％~122.7％。채용해방법고찰료타정대불동부위연초선협중안기화합물대사보적영향，결과현시타정처리대상부협영향최대，타정후상부협담대사주요류향생물감합성방향。해방법충분이용료삼중사겁간천련질보화고분변천련질보기술적우세，가실현식물제취액중안기대사물적고선택성、고령민도분석。
Amino metabolites are important compounds that play a key role in plant growth and development. A metabolic profiling analysis method of amino metabolites in plant extract was developed based on pre-column derivatization-ultra high performance liquid chromatography-mass spectrometry. Using the tobacco leaf as an example,a total of 87 amino metabolites, including amino acids,amines,peptides,alkaloids etc. were detected. The repeatability of the method was good with RSDs of 85 amino metabolites between 1. 5% and 18. 8%. Forty-three amino metabolites validated by standard samples showed good linearity with the correlation coefficients of 0. 993-0. 999,covered linear range of four orders of magnitude. The limits of detection were 0. 03-6. 58 ng/mL. The intra-day and inter-day precisions were 0. 7%-15. 6% and 0. 8%-22. 9%,respectively. The recoveries were 74. 4%-122. 7%. The influence of topping on metabolic profiling of amino metabolites in fresh tobacco was investigated using the developed method. The results showed that the amino metabolites in the upper tobacco leaves were most affected than those in the middle and lower leaves. Metabolism of amino metabolites in the upper leaves after topping was mainly towards the alkaloid synthesis. The method integrated the advantages of triple quadrupole mass spectrometry and high resolution quadrupole-time of flight mass spectrometry. It can be used for metabolic profiling analysis of amino metabolites in plant extract with high sensitivity and selectivity.