国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2015年
6期
532-534
,共3页
白芍%紫外分光光度法%白芍总苷%含量测定
白芍%紫外分光光度法%白芍總苷%含量測定
백작%자외분광광도법%백작총감%함량측정
Paeonia lactifloriapall.%UV spectrophotometry%Total glucosides of paeony%Quantitative determination
目的：建立紫外分光光度法测定白芍饮片中白芍总苷的含量。方法采用80%乙醇提取白芍饮片，提取液除去乙醇后，经乙醚萃取除去杂质，用正丁醇萃取制备供试品溶液。以芍药苷为对照品，在最大吸收波长230 nm处进行含量测定。结果芍药苷在0.0402～0.2011 mg/ml范围内线性关系良好。平均回收率为101.81%，精密度、稳定性、重复性、加样回收率试验中RSD值均小于2%。白芍中总苷含量约为40 mg/g。结论本方法简便、灵敏度高、准确性好，适用于白芍饮片中总苷的含量测定。
目的：建立紫外分光光度法測定白芍飲片中白芍總苷的含量。方法採用80%乙醇提取白芍飲片，提取液除去乙醇後，經乙醚萃取除去雜質，用正丁醇萃取製備供試品溶液。以芍藥苷為對照品，在最大吸收波長230 nm處進行含量測定。結果芍藥苷在0.0402～0.2011 mg/ml範圍內線性關繫良好。平均迴收率為101.81%，精密度、穩定性、重複性、加樣迴收率試驗中RSD值均小于2%。白芍中總苷含量約為40 mg/g。結論本方法簡便、靈敏度高、準確性好，適用于白芍飲片中總苷的含量測定。
목적：건립자외분광광도법측정백작음편중백작총감적함량。방법채용80%을순제취백작음편，제취액제거을순후，경을미췌취제거잡질，용정정순췌취제비공시품용액。이작약감위대조품，재최대흡수파장230 nm처진행함량측정。결과작약감재0.0402～0.2011 mg/ml범위내선성관계량호。평균회수솔위101.81%，정밀도、은정성、중복성、가양회수솔시험중RSD치균소우2%。백작중총감함량약위40 mg/g。결론본방법간편、령민도고、준학성호，괄용우백작음편중총감적함량측정。
ObjectiveA method was proposed for the determination of the concent of total glucoside of peaony(TGP) fromPaeonia lactifloriaPall. by UV spectrophotometry.MethodsBased on the extract with 80% acidities alcohol, extracted by aether and n-butyl alcohol, TGP was obtained fromPaeonialactifloriaPall.. The determination was established with paeoniflorin which was the main component of TGP as a contrast by UV spectrophotometry at 230 nm.ResultsA good liner relationship between concentrationof paeoniflorin and its absorbance was obtained,and the liner range was between 0.040 2~0.201 1 mg/ml. The averagerecovery was 101.81%.ConclusionThis method is simple rapid and reliable. It could be used for the semi-quantitative determination of TGP of paeony in paeonialactifloria pall..
