CAJ | 학술논문

建立了固相萃取-超高效液相色谱-串联质谱（ SPE-UPLC-MS／MS）同时测定食品接触塑料制品中10种苯并三唑类（ BZTs）紫外吸收剂的方法。食品接触塑料制品使用甲醇-二氯甲烷混合溶剂超声提取，C18固相萃取柱净化后用 Waters ACQUITY UPLC BEH C18色谱柱（100 mm×2.1 mm，1.7μm）分离，甲醇和0.1％（v／v）甲酸水溶液为流动相进行梯度洗脱，多反应监测（ MRM）模式检测，外标法定量分析。结果表明，10种 BZTs 在线性范围内线性关系良好，线性相关系数（ r2）均大于0.996；检出限为0.6~1.6μg／kg；3个水平下的加标回收率为75.2％~85.3％，相对标准偏差为1.0％~5.7％。该方法准确、简便、快速、检出限低，可用于食品接触塑料制品中10种 BZTs的同时检测。
건립료고상췌취-초고효액상색보-천련질보（ SPE-UPLC-MS／MS）동시측정식품접촉소료제품중10충분병삼서류（ BZTs）자외흡수제적방법。식품접촉소료제품사용갑순-이록갑완혼합용제초성제취，C18고상췌취주정화후용 Waters ACQUITY UPLC BEH C18색보주（100 mm×2.1 mm，1.7μm）분리，갑순화0.1％（v／v）갑산수용액위류동상진행제도세탈，다반응감측（ MRM）모식검측，외표법정량분석。결과표명，10충 BZTs 재선성범위내선성관계량호，선성상관계수（ r2）균대우0.996；검출한위0.6~1.6μg／kg；3개수평하적가표회수솔위75.2％~85.3％，상대표준편차위1.0％~5.7％。해방법준학、간편、쾌속、검출한저，가용우식품접촉소료제품중10충 BZTs적동시검측。
A sensitive method was developed for the simultaneous determination of ten benzo-triazole ultraviolet stabilizers in food contact plastic materials by ultra performance liquid chro-matography-tandem mass spectrometry( UPLC-MS/MS). The sample was extracted by metha-nol-dichloromethane,and purified by a C18 solid-phase extraction( SPE)column. The separa-tion was performed by using water containing 0. 1%( v/v ) formic acid and methanol as the mobile phases with gradient elution at a flow rate of 0. 3 mL/min. The electrospray ionization ( ESI)source in positive ion mode was used for the analysis of the ten benzotriazole ultraviolet stabilizers in multiple reaction monitoring( MRM)mode. The results showed that the standard curves were obtained with good correlation coefficients(r2﹥0. 996)in their linear concentration ranges. The limits of detection( LODs,S/N=3)for the ten benzotriazole ultraviolet stabilizers were in the range of 0. 6-1. 6 μg/kg. The mean recoveries for the ten benzotriazole ultraviolet stabilizers at three spiked levels( low,medium and high ) were 75. 2%-85. 3% with relative standard deviations of 1. 0%-5. 7%. Ten kinds of food contact plastic materials were tested,and 2,2′-methylenebis(6-(benzotriazol-2-yl)-4-tert-octylphenol)(UV-360)was found in a sample of polyethylene( PE ) material. The method is accurate,simple,rapid and feasible for the simultaneous determination of benzotriazole ultraviolet stabilizers in food plastic materials.