CAJ | 학술논문

为了实现蛋白质组的深度覆盖，特别是低丰度蛋白质的定性鉴定和定量分析，目前常用的方法是采用更长或装填更小粒径填料的毛细管色谱柱，但因此带来的问题是色谱柱反向柱压显著升高。针对以上问题发展了一种简单的毛细管色谱柱电加热装置制作方法，并将该装置安装于液相色谱-质谱联用系统，分别以牛血清白蛋白（ BSA）酶切肽段混合物和酵母蛋白（ yeast）酶切肽段混合物为样品，从柱压和柱效两方面对该装置的性能进行了评价。实验结果表明，所制作的毛细管柱电加热装置安装在装填粒径为3μm 反相色谱填料的毛细管柱上，在最佳电流（100 mA）下对 BSA及 yeast酶切肽段混合物进行分离时的柱压比不加电流时的柱压降低至少50％，柱效略有升高。这说明所制作的毛细管色谱柱电加热装置能显著降低柱压，为在较低的柱压条件下选择更小粒径色谱颗粒填料的毛细管色谱柱提供了一种有效的方法。
위료실현단백질조적심도복개，특별시저봉도단백질적정성감정화정량분석，목전상용적방법시채용경장혹장전경소립경전료적모세관색보주，단인차대래적문제시색보주반향주압현저승고。침대이상문제발전료일충간단적모세관색보주전가열장치제작방법，병장해장치안장우액상색보-질보련용계통，분별이우혈청백단백（ BSA）매절태단혼합물화효모단백（ yeast）매절태단혼합물위양품，종주압화주효량방면대해장치적성능진행료평개。실험결과표명，소제작적모세관주전가열장치안장재장전립경위3μm 반상색보전료적모세관주상，재최가전류（100 mA）하대 BSA급 yeast매절태단혼합물진행분리시적주압비불가전류시적주압강저지소50％，주효략유승고。저설명소제작적모세관색보주전가열장치능현저강저주압，위재교저적주압조건하선택경소립경색보과립전료적모세관색보주제공료일충유효적방법。
For deep coverage of proteome,especially in performing qualitative identification and quantitative analysis of low-abundance proteins,the most commonly used method is the application of a longer capillary chromatographic column or a capillary column packed with smaller particle sizes. However,this causes another problem,the very high back pressure which results in liquid leaks in some connection parts in a liquid chromatograph. To solve this problem,an electric heating apparatus was developed to raise the temperature of a capillary column for reducing its back pressure,which was further applied in a capillary high perform-ance liquid chromatography-tandem mass spectrometry system( cHPLC-MS/MS),and evalua-ted in the terms of chromatographic column back pressure and chromatographic column effi-ciency using bovine serum albumin( BSA)tryptic digests and yeast tryptic digests,separately. The results showed that at the optimum current,our electric heating apparatus could reduce the column pressure of a capillary column packed with 3 μm packing materials by at least 50%during the separation of BSA tryptic digestion and yeast tryptic digestion,compared with that without electric heating. The column efficiency was also increased slightly. This suggested that the electric heating apparatus can significantly reduce the column pressure,which provides an efficient way to use capillary chromatographic columns packed with smaller sizes of particles at a lower pressure.