茶叶科学
茶葉科學
다협과학
2015年
3期
233-238
,共6页
TF1%EGCG%Caco-2细胞模型%吸收规律
TF1%EGCG%Caco-2細胞模型%吸收規律
TF1%EGCG%Caco-2세포모형%흡수규률
TF1%EGCG%Caco-2 model%absorption regularity
为了探究茶黄素单体（TF1）与表没食子儿茶素没食子酸酯（EGCG）在生物体中的吸收规律，本实验建立了体外 Caco-2单层细胞模型，模拟小肠对 TF1与 EGCG的吸收，并研究了浓度和时间对吸收的影响。结果表明，在10~100μmol·L-1范围内，TF1与 EGCG 在 Caco-2单层细胞模型中的吸收呈现表观渗透系数 Papp随浓度增加而上升的规律。但两者的外排率也呈现同样的规律，并且外排率上升的幅度均大于二者吸收率上升的幅度。由于 TF1与 EGCG 在细胞单层模型中的 Papp 均小于1×10-6 cm·s -1，说明两者都属于难吸收的药物，但是TF1在Caco-2细胞模型中的吸收率高于EGCG。因其外排比均大于2，说明两者在细胞模型上的外排是被动转运。从吸收时间看，TF1的外排规律与EGCG一致。
為瞭探究茶黃素單體（TF1）與錶沒食子兒茶素沒食子痠酯（EGCG）在生物體中的吸收規律，本實驗建立瞭體外 Caco-2單層細胞模型，模擬小腸對 TF1與 EGCG的吸收，併研究瞭濃度和時間對吸收的影響。結果錶明，在10~100μmol·L-1範圍內，TF1與 EGCG 在 Caco-2單層細胞模型中的吸收呈現錶觀滲透繫數 Papp隨濃度增加而上升的規律。但兩者的外排率也呈現同樣的規律，併且外排率上升的幅度均大于二者吸收率上升的幅度。由于 TF1與 EGCG 在細胞單層模型中的 Papp 均小于1×10-6 cm·s -1，說明兩者都屬于難吸收的藥物，但是TF1在Caco-2細胞模型中的吸收率高于EGCG。因其外排比均大于2，說明兩者在細胞模型上的外排是被動轉運。從吸收時間看，TF1的外排規律與EGCG一緻。
위료탐구다황소단체（TF1）여표몰식자인다소몰식자산지（EGCG）재생물체중적흡수규률，본실험건립료체외 Caco-2단층세포모형，모의소장대 TF1여 EGCG적흡수，병연구료농도화시간대흡수적영향。결과표명，재10~100μmol·L-1범위내，TF1여 EGCG 재 Caco-2단층세포모형중적흡수정현표관삼투계수 Papp수농도증가이상승적규률。단량자적외배솔야정현동양적규률，병차외배솔상승적폭도균대우이자흡수솔상승적폭도。유우 TF1여 EGCG 재세포단층모형중적 Papp 균소우1×10-6 cm·s -1，설명량자도속우난흡수적약물，단시TF1재Caco-2세포모형중적흡수솔고우EGCG。인기외배비균대우2，설명량자재세포모형상적외배시피동전운。종흡수시간간，TF1적외배규률여EGCG일치。
In order to explore the absorption regularity of TF1 and EGCG in the organism. This research used the Caco-2 cell monolayer model in vitro to simulate the absorption of TF1 and EGCG in the small intestine. The influences of concentration and time on the absorption regularity of TF1 and EGCG in Caco-2 cell monolayer model were investigated. The results illustrated that the absorption of TF1 and EGCG in Caco-2 model showed that the apparent permeability coefficient raised with the increasing of concentrations of two compound in the range of 10~100 μ mol·L-1. The efflux rates of the TF1 and EGCG showed the same rules as absorption. However, the increasing range of efflux rate was higher than that of absorption rate. The values of Papp about TF 1 and EGCG in the cell model were lower than 1×10-6 cm·s -1, which indicated that both of them belonged to the kind of drugs which were difficult to absorb. However, with comparing the absorption rate, TF1 was higher than EGCG in this model. Both of efflux transport showed the passive process because the efflux rates of TF 1 and EGCG were larger than 2 and higher than in cell model. Because the efflux regulation of TF1 was in accordance with EGCG by temporal variation, it suggested that both of them were the substrate of the same efflux protein.
