CAJ | 학술논문

本研究提出了一种基于 HPLC 的茶叶黄酮苷类物质检测方法，采用此方法分析了西湖龙井茶中黄酮苷类物质在不同冲泡条件下的浸出特性，并通过Dot值（浓度与阈值的比值）分析黄酮苷类物质对茶汤滋味的影响。结果表明：（1）该检测方法可以较好地分离和测定茶叶中11种黄酮苷类物质；（2）传统冲泡条件下，西湖龙井茶中以杨梅素-3-O-半乳糖苷（Myr-gala）和槲皮素-3-O-芸香糖苷（Que-rut）为主，随着冲泡温度的提高和时间的延长，11种黄酮苷类物质除了山柰酚-3-O-芸香糖苷（Kae-rut）外，都呈不同程度的增加，其中槲皮素-3-O-芸香糖苷（Que-rut）和槲皮素-3-O-半乳糖苷（Que-gala）的浸出速率较快，而杨梅素-3-O-鼠李糖苷（Myr-rha）和牡荆素-2"-O-鼠李糖苷（Vit-rha）的浸出速率较慢；（3）通过Dot值分析，槲皮素-3-O-芸香糖苷（Que-rut）、槲皮素-3-O-半乳糖苷（Que-gala）和杨梅素-3-O-半乳糖苷（Myr-gala）的Dot值均高于10，可能是茶汤滋味的重要贡献物质。
본연구제출료일충기우 HPLC 적다협황동감류물질검측방법，채용차방법분석료서호룡정다중황동감류물질재불동충포조건하적침출특성，병통과Dot치（농도여역치적비치）분석황동감류물질대다탕자미적영향。결과표명：（1）해검측방법가이교호지분리화측정다협중11충황동감류물질；（2）전통충포조건하，서호룡정다중이양매소-3-O-반유당감（Myr-gala）화곡피소-3-O-예향당감（Que-rut）위주，수착충포온도적제고화시간적연장，11충황동감류물질제료산내분-3-O-예향당감（Kae-rut）외，도정불동정도적증가，기중곡피소-3-O-예향당감（Que-rut）화곡피소-3-O-반유당감（Que-gala）적침출속솔교쾌，이양매소-3-O-서리당감（Myr-rha）화모형소-2"-O-서리당감（Vit-rha）적침출속솔교만；（3）통과Dot치분석，곡피소-3-O-예향당감（Que-rut）、곡피소-3-O-반유당감（Que-gala）화양매소-3-O-반유당감（Myr-gala）적Dot치균고우10，가능시다탕자미적중요공헌물질。
The study proposed a standard analyzing method based on high performance liquid chromatography combined with ultraviolet (UV) to quantify extracting characteristics of flavone glycosides in Xihulongjing tea under different brewing conditions, and their contributions to taste was estimated by Dot factor (Dose-over-Threshold). Results indicated that: (1) This method can be used to separate and quantify eleven flavone and flavonol glycosides in tea; (2) In traditional brewing conditions, Myr-gala and Que-rut were the principal flavonol glycosides in Xihulongjing tea. With brewing temperature and time rising, eleven flavo ne and flavonol glycosides except Kae-rut increased variably with the raising of brewing temperature and the prolonging of brewing time, Que-rut and Que-gala leached fastly, while Myr-rha and Vit-rha leached slowly among 11 flavone and flavonol glycosides; (3)All of the Dot factors of Que-rut、Que-gala and Myr-gala were higher than 10. According to the definition of Dot factor, they may be showed a significant contributor to tea taste.