CAJ | 학술논문

目的：探讨粉防己碱对支气管哮喘(简称哮喘)小鼠肺组织核因子-κB(NF-κB)、诱导型一氧化氮合酶(iNOS)表达、气道炎症和气道高反应性的影响。方法将32只 SPF 级 BALB/c 小鼠随机分为正常组、哮喘组、地塞米松组(激素组)和粉防己碱组(Tet 组)。卵白蛋白(OVA)致敏和激发建立哮喘小鼠模型。末次激发24 h 后，肺功能仪测定小鼠气道阻力；HE 染色观察气道炎症细胞浸润；ELISA 检测血清总 IgE、OVA 特异性 IgE(OVA-sIgE)及 BALF 中 Th2细胞因子 IL-4和 IL-13水平；显微镜下计BALF 中细胞总数，瑞氏染色计嗜酸粒细胞分类计数；Western blot 检测肺组织 NF-κB 和 iNOS 蛋白表达水平。结果与正常组比较，哮喘组气道阻力、气道炎症浸润、BALF 炎症细胞总数和嗜酸粒细胞分类计数、血清总 IgE 和 OVA-sIgE、BALF 中 IL-4和 IL-13以及 NF-κB 和 iNOS 蛋白表达水平均显著增高(P <0.05)；与哮喘组比较，激素和 Tet 干预组上述各项指标均显著降低(P <0.05)。结论粉防己碱可下调哮喘小鼠肺组织 NF-κB 和 iNOS 表达并抑制气道炎症和气道高反应性。
목적：탐토분방기감대지기관효천(간칭효천)소서폐조직핵인자-κB(NF-κB)、유도형일양화담합매(iNOS)표체、기도염증화기도고반응성적영향。방법장32지 SPF 급 BALB/c 소서수궤분위정상조、효천조、지새미송조(격소조)화분방기감조(Tet 조)。란백단백(OVA)치민화격발건립효천소서모형。말차격발24 h 후，폐공능의측정소서기도조력；HE 염색관찰기도염증세포침윤；ELISA 검측혈청총 IgE、OVA 특이성 IgE(OVA-sIgE)급 BALF 중 Th2세포인자 IL-4화 IL-13수평；현미경하계BALF 중세포총수，서씨염색계기산립세포분류계수；Western blot 검측폐조직 NF-κB 화 iNOS 단백표체수평。결과여정상조비교，효천조기도조력、기도염증침윤、BALF 염증세포총수화기산립세포분류계수、혈청총 IgE 화 OVA-sIgE、BALF 중 IL-4화 IL-13이급 NF-κB 화 iNOS 단백표체수평균현저증고(P <0.05)；여효천조비교，격소화 Tet 간예조상술각항지표균현저강저(P <0.05)。결론분방기감가하조효천소서폐조직 NF-κB 화 iNOS 표체병억제기도염증화기도고반응성。
Objective To investigate the effect of tetrandrine (Tet)on the expression of nuclear factor-κB (NF-κB),inducible nitric oxide synthase (iNOS),airway inflammation and hyperresponsiveness in a murine model of bronchial asthma (asthma).Methods Thirty-two SPF BALB/c mice were randomly divided into 4 groups,including control group,asthma group,dexamethasone group(glucocorticoid group) and atetrandrine group (Tet group).Mice were sensitized and challenged by ovalbumin (OVA).Twenty-four hours after the last challenge,airway resistance was measured by pulmonary function detector. Hematoxylin & eosin (HE)staining was used to observe the airway inflammatory cells infiltration.Levels of total IgE and OVA-specific IgE (OVA-sIgE)in serum and Th2 cytokines (IL-4 and IL-13 ) in bronchoalveolar lavage fluid (BALF)were detected by enzyme-linked immunosorbent assay (ELISA). Total number of inflammatory cells in BALF were counted with a microscope.Smears of BALF cells were stained with Wright′s staining for eosinophils differential count.The protein expression of NF-κB and iNOS were determined by western blot analysis.Results Compared to the control group,the airway resistance,airway eosinophilia,total inflammatory cells and differential eosinophils count in BALF,total IgE and OVA-sIgE in serum,Th2 cytokines (IL-4 and IL-13)in BALF,as well as the protein expression of NF-κB and iNOS were significantly increased in asthma group (P < 0.05 ).In comparison with the asthma group,all the above indicators were remarkably decreased by treatment with either dexamethasone or tetrandrine (P < 0.05 ).Conclusions Tetrandrine may inhibit airway inflammation and hyperresponsiveness through downregulation of the expression of NF-κB and iNOS in asthmatic mice.