CAJ | 학술논문

目的：在情绪应激下心血管疾病复合模型大鼠构建的基础上，研究情绪和心血管疾病间的关系。通过观察、对比益气活血方对复合病模型大鼠心肌组织、脑海马体内脂联素含量的影响，为基础实验研究和临床心血管疾病防治、用药提供新的理论设想和依据。方法采取腹腔注射异丙肾上腺素合并慢性不可预见性情绪刺激法构建大鼠复合模型。将实验动物分为空白组、ISO模型组（单纯心肌缺血模型）、复合模型组（心肌缺血合并情志刺激模型）、中药组、西药组5组。采用超声心动图监测和行为学实验作为模型大鼠构建成功的依据。观察、对比大鼠心脏功能，行为学指标，心肌组织和脑海马体中脂联素各组用药后的变化情况。结果实验32d后，复合模型组和ISO模型组射血分数（EF）和短轴缩短率（FS）均低于空白组，差异有统计学意义（P<0.01）；复合模型组、ISO模型组心肌组织、脑海马体中的脂联素含量均低于空白组，差异有统计学意义（P<0.01或P<0.05）；中药组、西药组大鼠脂联素含量均高于复合模型组，差异有统计学意义（P<0.01或P<0.05）。结论益气活血药可以改善复合模型大鼠心脏功能和行为学指标，从而使模型大鼠机体走上良性循环道路，其原因可能与其对脂联素的作用有关。
목적：재정서응격하심혈관질병복합모형대서구건적기출상，연구정서화심혈관질병간적관계。통과관찰、대비익기활혈방대복합병모형대서심기조직、뇌해마체내지련소함량적영향，위기출실험연구화림상심혈관질병방치、용약제공신적이론설상화의거。방법채취복강주사이병신상선소합병만성불가예견성정서자격법구건대서복합모형。장실험동물분위공백조、ISO모형조（단순심기결혈모형）、복합모형조（심기결혈합병정지자격모형）、중약조、서약조5조。채용초성심동도감측화행위학실험작위모형대서구건성공적의거。관찰、대비대서심장공능，행위학지표，심기조직화뇌해마체중지련소각조용약후적변화정황。결과실험32d후，복합모형조화ISO모형조사혈분수（EF）화단축축단솔（FS）균저우공백조，차이유통계학의의（P<0.01）；복합모형조、ISO모형조심기조직、뇌해마체중적지련소함량균저우공백조，차이유통계학의의（P<0.01혹P<0.05）；중약조、서약조대서지련소함량균고우복합모형조，차이유통계학의의（P<0.01혹P<0.05）。결론익기활혈약가이개선복합모형대서심장공능화행위학지표，종이사모형대서궤체주상량성순배도로，기원인가능여기대지련소적작용유관。
Objective The purpose of this article is to through the animal experiment,study the effect of Yiqi-huoxue medicine on the emotional stress of myocardial ischemia model rats,adponectin contant in heart and brain,to provide a theoretical ideas and basis for Yiqi-huoxue medicine in prevention and treatment of cardiovascular diseases. Methods Adopt the method of intraperitoneal injection of epinephrine(5 mg/kg · d),and chronic unpredictability emotional stimuli to make complexsrats under emotional stress state. Animals are divided into five group:Blank group,ISO model group,Complex model group,Chinese medicine group,Western medicine groups. Using ultrasonic electrocardiogram,monitoring method to record the heart function,using the RT-PCR method for determinate the level of adponectin in heart and brain. According to these indexes of every group in the experimental process,respectively to observe contrast the changes of different groups after 14 and 28 days,treatment. Results After 32 days,the ejection fraction(EF)of complex model group,and fractional shortening(FS)were lower than those of blank group,the difference was statistically significant(P<0.01);adponectin contents in complex model group and ISO model group were higher than that in blank group,the difference was statistically significant(P<0.01 or P<0.05);adponectin contents in traditional Chinese medicine group and Western medicine group were lower than the complex model group,the difference was statistically significant(P<0.01 or P<0.05). Conclusion The reason why Yiqi-huoxue medicine can increase cardiac ejection fraction of rats in complex model group may be according to its effect on adponectin.