CAJ | 학술논문

对广东省粤西地区的5个荔枝品种进行核糖体DNA(rDNA)内转录间隔区(ITS)的PCR扩增，并用限制酶HhaI、Hae芋、EcoR玉、Hind芋、Rsa玉对扩增产物进行酶切分析。5种荔枝属植物的ITS基因序列长度为600~800 bp，PCR产物经过5种酶切后，5种荔枝属植物的PCR产物均切出位于600~800 bp之间的主带，妃子笑的主带与其余4种差异明显；结果表明，荔枝品种中的白糖罂、新兴、糯米糍、尚书怀4种的亲缘性比较近，妃子笑与这4种的亲缘性较远。
대광동성월서지구적5개려지품충진행핵당체DNA(rDNA)내전록간격구(ITS)적PCR확증，병용한제매HhaI、Hae우、EcoR옥、Hind우、Rsa옥대확증산물진행매절분석。5충려지속식물적ITS기인서렬장도위600~800 bp，PCR산물경과5충매절후，5충려지속식물적PCR산물균절출위우600~800 bp지간적주대，비자소적주대여기여4충차이명현；결과표명，려지품충중적백당앵、신흥、나미자、상서부4충적친연성비교근，비자소여저4충적친연성교원。
The PCR amplifications of ITS in Ribosomal DNA(rDNA)of five kinds of litchis grown in Guangdong Province were researched,using restriction enzyme HhaI,HaeⅢ,EcoR Ⅰ,HindⅢ,RsaⅠinto the enzyme digestion analysis.All these five kinds of litchis’ ITS genetic sequence lengths were 600~800 bp.After the enzyme digestion of PCR amplification,five kinds of litchis’ PCR amplifications were digested in the position of main belt between 600~800 bp.The litchis of Feizixiao were different from all other four spices in the main belt.The research results also revealed that the litchis of Baitangying,Xinxing,Nuomici and Shangshuhuai were relatively closed in the aspect of affinity,while the litchis of Feizixiao were more distant.