国际儿科学杂志
國際兒科學雜誌
국제인과학잡지
INTERNATIONAL JOURNAL OF PEDIATRICS
2015年
3期
331-333,337
,共4页
顾卉%黄天楚%梅妍%袁正伟
顧卉%黃天楚%梅妍%袁正偉
고훼%황천초%매연%원정위
微小RNA%神经管缺陷%产前诊断%胎儿
微小RNA%神經管缺陷%產前診斷%胎兒
미소RNA%신경관결함%산전진단%태인
MicroRNA%Neural tube defect%Prenatal diagnosis%Fetus
目的 母亲血清中微小RNA(microRNA,miRNA)的发现为无创性产前诊断开辟了新途径.但是对神经管缺陷胎儿母亲血清中妊娠相关的miRNA的研究甚少.该文旨在研究微小RNA-423(mi-croRNA-423,miR-423)在神经管缺陷胎儿孕妇血清中的异常表达及其作为潜在诊断标志物的临床价值.方法 33例产前超声检查确诊为胎儿神经管缺陷的患儿为研究对象,其中脊柱裂22例,无脑儿11例;33例胎儿健康孕妇为对照组.所有孕妇均于清晨空腹抽外周静脉血5ml离心后取血清,提取血清总RNA,用Real-time RT-PCR方法测定miR-423表达水平.并用ROC曲线分析用miR-423诊断胎儿神经管缺陷的价值.结果 神经管缺陷胎儿孕妇血清中miR-423含量(0.96±0.14)明显低于健康胎儿孕妇对照组(2.28±0.43),P＜0.05.ROC分析miR-423曲线下面积为0.711(95％ CI:0.566～0.856)(P＜0.05).另外,对不同类型的神经管缺陷孕妇血清中的miR-423表达水平分析发现,只有在无脑儿中表达降低(0.58±0.08)差异有统计学意义.结论 孕妇血清中miR-423可作为胎儿神经管缺陷的无创性产前诊断标志物,具有潜在的临床价值,可能预示胎儿神经管缺陷严重程度.
目的 母親血清中微小RNA(microRNA,miRNA)的髮現為無創性產前診斷開闢瞭新途徑.但是對神經管缺陷胎兒母親血清中妊娠相關的miRNA的研究甚少.該文旨在研究微小RNA-423(mi-croRNA-423,miR-423)在神經管缺陷胎兒孕婦血清中的異常錶達及其作為潛在診斷標誌物的臨床價值.方法 33例產前超聲檢查確診為胎兒神經管缺陷的患兒為研究對象,其中脊柱裂22例,無腦兒11例;33例胎兒健康孕婦為對照組.所有孕婦均于清晨空腹抽外週靜脈血5ml離心後取血清,提取血清總RNA,用Real-time RT-PCR方法測定miR-423錶達水平.併用ROC麯線分析用miR-423診斷胎兒神經管缺陷的價值.結果 神經管缺陷胎兒孕婦血清中miR-423含量(0.96±0.14)明顯低于健康胎兒孕婦對照組(2.28±0.43),P＜0.05.ROC分析miR-423麯線下麵積為0.711(95％ CI:0.566～0.856)(P＜0.05).另外,對不同類型的神經管缺陷孕婦血清中的miR-423錶達水平分析髮現,隻有在無腦兒中錶達降低(0.58±0.08)差異有統計學意義.結論 孕婦血清中miR-423可作為胎兒神經管缺陷的無創性產前診斷標誌物,具有潛在的臨床價值,可能預示胎兒神經管缺陷嚴重程度.
목적 모친혈청중미소RNA(microRNA,miRNA)적발현위무창성산전진단개벽료신도경.단시대신경관결함태인모친혈청중임신상관적miRNA적연구심소.해문지재연구미소RNA-423(mi-croRNA-423,miR-423)재신경관결함태인잉부혈청중적이상표체급기작위잠재진단표지물적림상개치.방법 33례산전초성검사학진위태인신경관결함적환인위연구대상,기중척주렬22례,무뇌인11례;33례태인건강잉부위대조조.소유잉부균우청신공복추외주정맥혈5ml리심후취혈청,제취혈청총RNA,용Real-time RT-PCR방법측정miR-423표체수평.병용ROC곡선분석용miR-423진단태인신경관결함적개치.결과 신경관결함태인잉부혈청중miR-423함량(0.96±0.14)명현저우건강태인잉부대조조(2.28±0.43),P＜0.05.ROC분석miR-423곡선하면적위0.711(95％ CI:0.566～0.856)(P＜0.05).령외,대불동류형적신경관결함잉부혈청중적miR-423표체수평분석발현,지유재무뇌인중표체강저(0.58±0.08)차이유통계학의의.결론 잉부혈청중miR-423가작위태인신경관결함적무창성산전진단표지물,구유잠재적림상개치,가능예시태인신경관결함엄중정도.
Objective The discovery of microRNA (miRNA) in maternal serum has opened up new possibilities for non-invasive prenatal diagnosis.However,our understanding of these pregnancy-related miRNA in the serum of pregnant women with fetuses with neural tube defects (NTDs) is still limited.This article is to study the dysregulated expression of microRNA-423 (miR-423) in the serum of pregnant women with neural tube defect(NTD) fetuses and its potential role as a biomarker for non-invasive prenatal diagnosis of fetal NTD.Methods Thirty-three pregnant women whose fetuses were diagnosed as neural tube defects by ultrasound (22 cases of spina bifida and 11 cases of anencephaly)and 33 normal pregnant women were selected.Peripheral venous blood of each pregnant woman was obtained early in the morning,the serum was purified from blood by centrifugation,then total RNA was isolated from serum and the miR-423 levels were detected by real-time RT-PCR.The ROC curve was used for assessing the diagnostic accuracy of miR-423 for fetal NTD.Results We revealed miR-423 with signifcant down-regulation in expression in serum of pregnant women with NTD fetuses (0.96 ±0.14) compared as women with normal pregnancies(2.28 ±0.43) (P ＜0.05).We performed ROC analysis of data from the 33 case-control pairs.The expression of miR-423 could distinguish NTD cases from normal controls,with an AUC of 0.711 (95 ％ CI:0.566 ～ 0.856) (P ＜ 0.05).Moreover,the expression of miR423 decreased only in serum of pregnant women with anencephaly fetuses(0.58 ±0.08)by the analysis in different forms of NTD.Conclusion miR-423 is deregulated in the serum of pregnant women with NTD fetuses and highlight the clinical potential of miR-423 as biomarker for diagnosis and prognostication of fetal NTD.
