CAJ | 학술논문

目的：比较在体外培养下下颌骨髁突软骨与股骨头软骨的生长发育状况。方法：取新生小鼠的髁突软骨和股骨头软骨进行组织培养，在培养前和培养6周后进行大体观察、HE 染色、茜素红染色以及 PCNA 免疫组化研究。结果：大体观察可以看到髁突软骨培养后，软骨内发生了异常改变，而软骨面积几乎没有变化（P ＞0．05）；HE 染色显示部分软骨层结构消失，茜素红染色证实了软骨基质发生了钙化。股骨头软骨培养后，软骨内尚未观察到异常，而软骨面积增大明显（P ＜0．05）；HE 染色显示肥大细胞层增厚，茜素红染色显示股骨头软骨基质尚未出现钙化。PCNA 免疫组化研究均提示两类软骨在培养后依然具有增殖能力。结论：在体外培养下，髁突软骨基质可以自发钙化。
목적：비교재체외배양하하합골과돌연골여고골두연골적생장발육상황。방법：취신생소서적과돌연골화고골두연골진행조직배양，재배양전화배양6주후진행대체관찰、HE 염색、천소홍염색이급 PCNA 면역조화연구。결과：대체관찰가이간도과돌연골배양후，연골내발생료이상개변，이연골면적궤호몰유변화（P ＞0．05）；HE 염색현시부분연골층결구소실，천소홍염색증실료연골기질발생료개화。고골두연골배양후，연골내상미관찰도이상，이연골면적증대명현（P ＜0．05）；HE 염색현시비대세포층증후，천소홍염색현시고골두연골기질상미출현개화。PCNA 면역조화연구균제시량류연골재배양후의연구유증식능력。결론：재체외배양하，과돌연골기질가이자발개화。
Objective:To study the difference of the development between mandibular condylar cartilage and femoral head cartilage in vitro.Methods:Mandibular condyles and femoral heads were sampled from 1 2 neonatal mice and cultured in vitro.The samples before culture and after 6-week culture were examined by gross observation,HE staining,Alizarin Red staining and PCNA immunohistochem-istry respectively.Results:After in vitro culture,abnormal changes were observed in condyle cartilage,but the surface area of condyle cartilage was not changed(P >0.05).HE staining showed partial cartilage layer structure disappearance and the Alizarin Red staining confirmed calcification in the cartilage matrix.However,calcification was not found in femoral head cartilage,and the surface area of femoral head cartilage increased(P <0.05).HE staining showed the hypertrophied layer was thicker after culture than before and the Alizarin Red staining showed there was no calcification in the femoral cartilage matrix.The immunohistochemistry displayed PCNA posi-tive expression in both cartilage after culture.Conclusion:In vitro,the mandibular condylar cartilage matrix can be spontaneously cal-cificated.