CAJ | 학술논문

目的：评价包载 PTH（1-34）聚乳酸微球的缓释效应对前成骨细胞 MC3T3-E1成骨分化的影响。方法：将 MC3T3-E1细胞分为空白培养液对照组、持续性和间歇性给药组、未载药 PLGA 微球组和载药 PLGA 微球组。通过碱性磷酸酶（ALP）染色、茜素红染色和 Reverse Transcription PCR 观察负载 PTH（1-34）的 PLGA 微球对细胞成骨分化的影响。结果：释放终浓度为10－9 mol／L 的载药 PLGA 微球组可以提高成骨细胞 ALP 活性，促进矿化，上调 RUNX2、ALP 和 VEGF 基因的表达。结论：PLGA微球缓释 PTH（1-34）可促进成骨细胞分化。
목적：평개포재 PTH（1-34）취유산미구적완석효응대전성골세포 MC3T3-E1성골분화적영향。방법：장 MC3T3-E1세포분위공백배양액대조조、지속성화간헐성급약조、미재약 PLGA 미구조화재약 PLGA 미구조。통과감성린산매（ALP）염색、천소홍염색화 Reverse Transcription PCR 관찰부재 PTH（1-34）적 PLGA 미구대세포성골분화적영향。결과：석방종농도위10－9 mol／L 적재약 PLGA 미구조가이제고성골세포 ALP 활성，촉진광화，상조 RUNX2、ALP 화 VEGF 기인적표체。결론：PLGA미구완석 PTH（1-34）가촉진성골세포분화。
Objective:To determin the effect of PLGA microspheres loading with PTH(1 34)[PTH(1 34)/PLGA]on the differentiation of MC3T3E1 cells.Methods:MC3T3E1 cells were divided into control group,continuous or intermittent PTH(1 34)adminstration groups,PLGA microsphere group and PTH(1 34)/PLGA group.Osteogenesis differentiation was observed by alkaline phosphatase activity(ALP),alizarin red staining and RTPCR.Results:The PTH(1 34)/PLGA with 1 0 -9 mol/L final release concentration enhanced ALP activity and mineralization,increased the mRNA expression of RUNX2,ALP and VEGF.Conclusion:Controlledrelease of PTH(1 34)from PLGA microspheres can promote the osteogenesis differentiation of MC3T3E1 cells.