CAJ | 학술논문

目的：探讨阻断降钙素基因相关肽（CGRP）受体活性修饰蛋白（RAMP1）在 MG-63细胞中表达对促成骨样 MG-63细胞增殖作用的影响。方法：体外转录合成法合成及筛选 RAMP1 siRNA，传代培养的 MG-63细胞分为：RAMP1 siRNA 干扰组、空载体组、空白对照组。实时聚合酶链反应、免疫荧光法分别检测降钙素受体样受体（CRLR）、受体组分蛋白（RCP）在 MG-63细胞中 mRNA 表达及膜上的分布变化。结果：转染 siRNA 后 MG-63细胞 RAMP1、CRLR mRNA 和荧光强度明显下调（P ＜0．05），RAMP1干扰组 RCP mRNA 的表达及荧光强度高于其它2组（P ＜0．05）。RAMP1 siRNA 干扰后，MG-63细胞的增殖被抑制（P ＜0．05）。结论：转染 RAMP1 siRNA 降低了 MG-63细胞 CRLR 的表达，抑制了 MG-63细胞的增殖能力。
목적：탐토조단강개소기인상관태（CGRP）수체활성수식단백（RAMP1）재 MG-63세포중표체대촉성골양 MG-63세포증식작용적영향。방법：체외전록합성법합성급사선 RAMP1 siRNA，전대배양적 MG-63세포분위：RAMP1 siRNA 간우조、공재체조、공백대조조。실시취합매련반응、면역형광법분별검측강개소수체양수체（CRLR）、수체조분단백（RCP）재 MG-63세포중 mRNA 표체급막상적분포변화。결과：전염 siRNA 후 MG-63세포 RAMP1、CRLR mRNA 화형광강도명현하조（P ＜0．05），RAMP1간우조 RCP mRNA 적표체급형광강도고우기타2조（P ＜0．05）。RAMP1 siRNA 간우후，MG-63세포적증식피억제（P ＜0．05）。결론：전염 RAMP1 siRNA 강저료 MG-63세포 CRLR 적표체，억제료 MG-63세포적증식능력。
Objective:To investigate the effect of blocking the expression of receptor activity modifying protein 1 (RAMP1 )on calcito-nin gene-related peptide(CGRP)-induced MG-63 cell proliferation.Methods:RAMP1 siRNA was synthesized and screened by tran-scription in vitro.The subcultured MG-63 cells were divided into the following groups:RAMP1 siRNA interference group,empty vector group and blank control group.The mRNA expression and the membrane distribution changes of the calcitonin receptor-like receptor (CRLR)and the receptor component protein (RCP)in MG-63 cells were examined by real-time PCR and immunofluorescence method respectively.Results:RAMP1 and CRLR mRNA and the fluorescence intensity of MG-63 cells decreased after transfection by RAMP1 siRNA(P <0.05).In RAMP1 interference group,the expression of RCP mRNA and the fluorescence intensity were higher than those in the other two groups(P <0.05).After RAMP1 siRNA interference,the proliferation of MG-63 cells was inhibited(P <0.05). Conclusion:RAMP1 siRNA transfection may reduce CRLR expression and inhibite the proliferation of MG-63 cell.