陕西中医
陝西中醫
협서중의
SHAANXI JOURNAL OF TRADITIONAL CHINESE MEDICINE
2015年
6期
753-755
,共3页
郝改梅%韩静%徐涛%何洁%吴晏%武志黔%袁悦莹%吕甜甜%王宏亮%王伟%毕力夫
郝改梅%韓靜%徐濤%何潔%吳晏%武誌黔%袁悅瑩%呂甜甜%王宏亮%王偉%畢力伕
학개매%한정%서도%하길%오안%무지검%원열형%려첨첨%왕굉량%왕위%필력부
糖尿病神经病变/中医药疗法%动物实验%大鼠%复方血栓通胶囊
糖尿病神經病變/中醫藥療法%動物實驗%大鼠%複方血栓通膠囊
당뇨병신경병변/중의약요법%동물실험%대서%복방혈전통효낭
Diabetic peripheral neuropathy/traditional Chinese medicine therapy%Animal experimentationt Rat%Xueshuantong capsule
目的：观察复方血栓通胶囊对糖尿病大鼠周围神经病变的疗效并探讨其作用机制。方法：选取SD大鼠32只，禁食12 h ，一次性腹腔注射链脲佐菌素（65 mg／kg ）诱导糖尿病模型，造模后将大鼠随机分为模型组和复方血栓通胶囊组。另选取11只大鼠做为正常对照组。每组每天干预1次，12周后检测大鼠的热痛潜伏期、神经血流量、微循环血流灌注量及神经传导速度，并观察坐骨神经病理变化。结果：与正常组比较，模型组大鼠热痛潜伏期延长，神经血流量减少，神经传导速度减慢；髓鞘松解；耳部及上肢末端微循环血流灌注量明显减少；下肢末端血流灌注量减少。与模型组比较，复方血栓通胶囊组大鼠热痛潜伏期缩短，神经血流量增大，神经传导速度增快，神经束膜整齐，耳部及上肢末端微循环血流灌注量明显增加，下肢末端血流灌注量增加。结论：复方血栓通可能是通过增加周围神经组织的微循环灌注量，对糖尿病大鼠周围神经病变进行改善。
目的：觀察複方血栓通膠囊對糖尿病大鼠週圍神經病變的療效併探討其作用機製。方法：選取SD大鼠32隻，禁食12 h ，一次性腹腔註射鏈脲佐菌素（65 mg／kg ）誘導糖尿病模型，造模後將大鼠隨機分為模型組和複方血栓通膠囊組。另選取11隻大鼠做為正常對照組。每組每天榦預1次，12週後檢測大鼠的熱痛潛伏期、神經血流量、微循環血流灌註量及神經傳導速度，併觀察坐骨神經病理變化。結果：與正常組比較，模型組大鼠熱痛潛伏期延長，神經血流量減少，神經傳導速度減慢；髓鞘鬆解；耳部及上肢末耑微循環血流灌註量明顯減少；下肢末耑血流灌註量減少。與模型組比較，複方血栓通膠囊組大鼠熱痛潛伏期縮短，神經血流量增大，神經傳導速度增快，神經束膜整齊，耳部及上肢末耑微循環血流灌註量明顯增加，下肢末耑血流灌註量增加。結論：複方血栓通可能是通過增加週圍神經組織的微循環灌註量，對糖尿病大鼠週圍神經病變進行改善。
목적：관찰복방혈전통효낭대당뇨병대서주위신경병변적료효병탐토기작용궤제。방법：선취SD대서32지，금식12 h ，일차성복강주사련뇨좌균소（65 mg／kg ）유도당뇨병모형，조모후장대서수궤분위모형조화복방혈전통효낭조。령선취11지대서주위정상대조조。매조매천간예1차，12주후검측대서적열통잠복기、신경혈류량、미순배혈류관주량급신경전도속도，병관찰좌골신경병리변화。결과：여정상조비교，모형조대서열통잠복기연장，신경혈류량감소，신경전도속도감만；수초송해；이부급상지말단미순배혈류관주량명현감소；하지말단혈류관주량감소。여모형조비교，복방혈전통효낭조대서열통잠복기축단，신경혈류량증대，신경전도속도증쾌，신경속막정제，이부급상지말단미순배혈류관주량명현증가，하지말단혈류관주량증가。결론：복방혈전통가능시통과증가주위신경조직적미순배관주량，대당뇨병대서주위신경병변진행개선。
Objective:To observe the effect of compound Xueshuantong(XST) capsule on diabetic peripheral neu‐ropathy(DPN) in rats and to explore its mechanism of action .Methods :32 SD rats were selected and fasted for 12h . Streptozotocin(STZ ,65mg/kg) was intraperitoneally injected to rats to induce diabetes .The diabetic rats were randomly divided into the model group and the XST group .Other 11 rats were recruited as the normal control group .The interven‐tion was carried out once daily and continued for 12 weeks .The threshold of hot pain test ,nerve blood flow ,microcircu‐latory perfusion ,nerve conduction velocity and pathological changes were observed .Results:Compared with the normal group ,the latency of hot pain was delayed ,the nerve blood flow was reduced and the conduction velocity was decreased in the model group .Myelin sheaths was loose .The microcirculatory perfusion of ear and upper end was decreased;while the perfusion of lower extremities reduced .Compared with the model group ,the latency of hot pain was shortened ,the nerve blood flow was reduced and the conduction velocity was increased in XST group .The perineurium was in order .The mi‐crocirculatory perfusion of ear and upper end was increase ,while the perfusion of lower extremities induced .Conclusion :XST can improve the DPN in rats by increasing the microcirculatory perfusion of peripheral nerve tissue .
