中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2015年
5期
329-332
,共4页
徐安平%夏勇%纪玲%喻晶%周宇%李璐
徐安平%夏勇%紀玲%喻晶%週宇%李璐
서안평%하용%기령%유정%주우%리로
β地中海贫血%血红蛋白A ,糖基化%血红蛋白A2%毛细管电泳法
β地中海貧血%血紅蛋白A ,糖基化%血紅蛋白A2%毛細管電泳法
β지중해빈혈%혈홍단백A ,당기화%혈홍단백A2%모세관전영법
β-thalassemia%Hemoglobin A,glycosylated%Hemoglobin A2%Capillary electrophoresis
目的探讨β地中海贫血对毛细管电泳法等8种糖化血红蛋白检测系统的影响，以及毛细管电泳糖化血红蛋白系统检测β地中海贫血中HbA2的应用。方法收集2014年1至8月北京大学深圳医院确诊的非β地中海贫血的样本338例，其中正常样本258名，α地中海贫血80例（基因型为－－SEA／αα）；以及β地中海贫血且不合并其他血红蛋白病样本（α珠蛋白基因正常，β珠蛋白基因异常且为杂合子）225例。采用毛细管电泳法（ Capillary 2 Flex Piercing）、酶法（ Norudia N HbA1c ）、免疫透射比浊法（ one HbA1c FS）、化学发光免疫法（ ARCHITECT HbA1c Reagent Kit）、亲和层析高压液相色谱法（Ultra2）和离子交换高压液相色谱法（HA8160、Variant ⅡTurbo和Variant ⅡTurbo 2．0），对正常和β地中海贫血各76例样本进行HbA1c检测，比较不同检测系统HbA1c结果的相关性。毛细管电泳糖化血红蛋白检测仪和毛细管电泳血红蛋白检测仪分别检测338例非β地中海贫血样本和225例β地中海贫血样本的HbA2，通过ROC曲线计算毛细管电泳糖化血红蛋白检测仪HbA2，建立筛查β地中海贫血的截断值。结果对于正常样本，7种检测系统与参比系统（ Ultra2）的相关性良好，相关系数都＞0．98；对于β地中海贫血样本，除Variant ⅡTurbo与参比系统相关系数为0．972外，其余检测系统相关系数都＞0．98。除Variant ⅡTurbo外，正常样本和β地中海贫血样本HbA1c结果的偏倚差异无统计学意义。毛细管电泳糖化血红蛋白检测系统和毛细管电泳血红蛋白检测系统的HbA2有良好的相关性，相关系数为0．993。毛细管电泳糖化血红蛋白检测系统HbA2筛查β地中海贫血的截断值设定为3．38时，敏感性为98．2％，特异性为100％。结论除Variant ⅡTurbo外，β地中海贫血对于其他糖化血红蛋白检测系统无明显影响。毛细管电泳糖化血红蛋白检测系统在检测糖化血红蛋白的同时，具有筛查β地中海贫血的功能。（中华检验医学杂志，2015，38：329－332）
目的探討β地中海貧血對毛細管電泳法等8種糖化血紅蛋白檢測繫統的影響，以及毛細管電泳糖化血紅蛋白繫統檢測β地中海貧血中HbA2的應用。方法收集2014年1至8月北京大學深圳醫院確診的非β地中海貧血的樣本338例，其中正常樣本258名，α地中海貧血80例（基因型為－－SEA／αα）；以及β地中海貧血且不閤併其他血紅蛋白病樣本（α珠蛋白基因正常，β珠蛋白基因異常且為雜閤子）225例。採用毛細管電泳法（ Capillary 2 Flex Piercing）、酶法（ Norudia N HbA1c ）、免疫透射比濁法（ one HbA1c FS）、化學髮光免疫法（ ARCHITECT HbA1c Reagent Kit）、親和層析高壓液相色譜法（Ultra2）和離子交換高壓液相色譜法（HA8160、Variant ⅡTurbo和Variant ⅡTurbo 2．0），對正常和β地中海貧血各76例樣本進行HbA1c檢測，比較不同檢測繫統HbA1c結果的相關性。毛細管電泳糖化血紅蛋白檢測儀和毛細管電泳血紅蛋白檢測儀分彆檢測338例非β地中海貧血樣本和225例β地中海貧血樣本的HbA2，通過ROC麯線計算毛細管電泳糖化血紅蛋白檢測儀HbA2，建立篩查β地中海貧血的截斷值。結果對于正常樣本，7種檢測繫統與參比繫統（ Ultra2）的相關性良好，相關繫數都＞0．98；對于β地中海貧血樣本，除Variant ⅡTurbo與參比繫統相關繫數為0．972外，其餘檢測繫統相關繫數都＞0．98。除Variant ⅡTurbo外，正常樣本和β地中海貧血樣本HbA1c結果的偏倚差異無統計學意義。毛細管電泳糖化血紅蛋白檢測繫統和毛細管電泳血紅蛋白檢測繫統的HbA2有良好的相關性，相關繫數為0．993。毛細管電泳糖化血紅蛋白檢測繫統HbA2篩查β地中海貧血的截斷值設定為3．38時，敏感性為98．2％，特異性為100％。結論除Variant ⅡTurbo外，β地中海貧血對于其他糖化血紅蛋白檢測繫統無明顯影響。毛細管電泳糖化血紅蛋白檢測繫統在檢測糖化血紅蛋白的同時，具有篩查β地中海貧血的功能。（中華檢驗醫學雜誌，2015，38：329－332）
목적탐토β지중해빈혈대모세관전영법등8충당화혈홍단백검측계통적영향，이급모세관전영당화혈홍단백계통검측β지중해빈혈중HbA2적응용。방법수집2014년1지8월북경대학심수의원학진적비β지중해빈혈적양본338례，기중정상양본258명，α지중해빈혈80례（기인형위－－SEA／αα）；이급β지중해빈혈차불합병기타혈홍단백병양본（α주단백기인정상，β주단백기인이상차위잡합자）225례。채용모세관전영법（ Capillary 2 Flex Piercing）、매법（ Norudia N HbA1c ）、면역투사비탁법（ one HbA1c FS）、화학발광면역법（ ARCHITECT HbA1c Reagent Kit）、친화층석고압액상색보법（Ultra2）화리자교환고압액상색보법（HA8160、Variant ⅡTurbo화Variant ⅡTurbo 2．0），대정상화β지중해빈혈각76례양본진행HbA1c검측，비교불동검측계통HbA1c결과적상관성。모세관전영당화혈홍단백검측의화모세관전영혈홍단백검측의분별검측338례비β지중해빈혈양본화225례β지중해빈혈양본적HbA2，통과ROC곡선계산모세관전영당화혈홍단백검측의HbA2，건립사사β지중해빈혈적절단치。결과대우정상양본，7충검측계통여삼비계통（ Ultra2）적상관성량호，상관계수도＞0．98；대우β지중해빈혈양본，제Variant ⅡTurbo여삼비계통상관계수위0．972외，기여검측계통상관계수도＞0．98。제Variant ⅡTurbo외，정상양본화β지중해빈혈양본HbA1c결과적편의차이무통계학의의。모세관전영당화혈홍단백검측계통화모세관전영혈홍단백검측계통적HbA2유량호적상관성，상관계수위0．993。모세관전영당화혈홍단백검측계통HbA2사사β지중해빈혈적절단치설정위3．38시，민감성위98．2％，특이성위100％。결론제Variant ⅡTurbo외，β지중해빈혈대우기타당화혈홍단백검측계통무명현영향。모세관전영당화혈홍단백검측계통재검측당화혈홍단백적동시，구유사사β지중해빈혈적공능。（중화검험의학잡지，2015，38：329－332）
Objective To investigate effects of β-thalassemia on 8 HbA1c measurement systems including Capillary electrophoresis and application of HbA2 values of samples with β-thalassemia from Capillary 2 Flex Piercing HbA1c measurement systems.Methods 338 samples without β-thalassemia ( include 80 α-thalassemia with --SEA/ααgenotype) and 225 samples with β-thalassemia were collected in Peking University Shenzhen hospital from January 2014 to August 2014.Samples withβ-thalassemia are all heterozygotes with normal α-globin and abnormal β-globin, and without combining other haemoglobinopathies.76 normal samples and 76 samples with β-thalassemia were selected from above all samples, HbA1c values of which were given by capillary electrophoresis ( Capillary Flex 2 Piercing), enzymatic ( Norudia N HbA1c ), immune transmission turbidity ( oneHbA1c FS ), chemiluminescent immunoassay (ARCHITECT HbA1c Reagent Kit), boronate affinity HPLC (Trinity Ultra2)and ion exchange HPLC (HA8160, VariantⅡTurbo and Variant Ⅱ BioRad 2.0), respectively.HbA1c values given by the eight systems were compared.338 samples without β-thalassemia and 225 samples with β-thalassemia were measured by using Capillarys 2 Flex Piercing HbA1c system and capillary electrophoresis hemoglobin system, the cut-off values of HbA2 from Capillary 2 Flex Piercing HbA1c measurement system for β-thalassemia was <br> assessed by using ROC curve.Data statistics were done by using SPSS 19.0.Results The HbA1c values of normal samples were well correlated with the comparative system.The correlation coefficients for the 7 systems are all greater than 0.98.For samples withβ-thalassemia, the correlation coefficients are all greater than 0.98 except for Variant Ⅱ Turbo.The bias between normal samples and samples with β-thalassemia have no significant difference except for VariantⅡTurbo.HbA2 values from Capillary 2 Flex Piercing HbA1c measurement and capillary electrophoresis hemoglobin system were well correlated. The correlation coefficient is 0.993.When cut-off value of HbA2 from Capillary 2 Flex Piercing HbA1c measurement system for β-thalassemia is 3.38, sensitivity is 98.3%, specificity is 100% .Conclusions Samples withβ-thalassemia have no significant effects on HbA1c measurement syetems except for Variant Ⅱ Turbo.The Capillary 2 Flex Piercing HbA1c measurement syetem has the advantage of screeningβthalassemia by HbA2 while measuring HbA1c.
