中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2015年
5期
341-345
,共5页
邵晓晓%林心心%郑金珏%林秀清%姜利佳%郑亮%李士林%郑超%蒋益
邵曉曉%林心心%鄭金玨%林秀清%薑利佳%鄭亮%李士林%鄭超%蔣益
소효효%림심심%정금각%림수청%강리가%정량%리사림%정초%장익
结肠炎,溃疡性%受体, TNF相关凋亡诱导配体%多态性,单核苷酸
結腸炎,潰瘍性%受體, TNF相關凋亡誘導配體%多態性,單覈苷痠
결장염,궤양성%수체, TNF상관조망유도배체%다태성,단핵감산
Colitis,ulcerative%Receptors,TNF-related apoptosis-inducing ligand%Polymorphism,single nucleotide
目的:探讨TRAIL受体基因多态性与溃疡性结肠炎( UC)的关系。方法从2008年1月至2012年12月从温州市4家大型综合性医院共收集380例UC患者[男215例,女165例,平均年龄(42.63±14.61)岁]和539名健康对照者[男290名,女249名,平均年龄(41.29±15.86)岁]。采用微测序技术检测 DR4( rs20575、rs13278062), DR5( rs1047266), DcR2( rs1133782)及 OPG (rs3102735)5种单核苷酸多态性(SNP)。采用χ2检验或Fisher精确概率法在UC组和对照组之间比较各SNP的等位基因及基因型频率差异,非条件Logistic回归分析5种SNP与UC患者临床病理特征的关系,Logistic多元回归分析UC的危险因素及因素间的相乘交互作用。结果与对照组比较, UC组中DR4(rs20575)的突变等位基因(G)和基因型(CG+GG)频率均增高(3.55%比1.95%,χ2=4.512,P=0.034;6.58%比3.71%,χ2=3.938,P=0.047),而DcR2(rs1133782)的突变等位基因(A)和基因型(GA+AA)频率均降低(6.18%比9.09%,χ2=5.183,P=0.023;11.32%比17.44%,χ2=6.589,P=0.010)。 UC组中OPG(rs3102735)的突变等位基因(T)和基因型(TT)频率均显著高于对照组(86.32%比81.54%,χ2=7.385,P=0.007;75.26%比66.98%,χ2=7.346,P=0.007)。 DcR2(rs1133782)野生基因型(GG)是 UC 的独立危险因素(OR =4.937,95%CI:2.320~10.504,P <0.001);DR4(rs20575)基因型(CC)分别与DcR2(rs1133782)基因型(GG)和OPG(rs3102735)基因型(TT)之间存在相乘交互作用(OR=0.322,95%CI:0.164~0.633,P=0.001; OR=1.580,95%CI:1.165~2.144,P=0.003)。结论 DR4(rs20575),DcR2(rs1133782)及OPG(rs3102735)基因多态性与UC相关。 DcR2(rs1133782)基因突变可能是UC的保护因素。 DR4(rs20575)分别与DcR2(rs1133782)和OPG( rs3102735)存在交互作用,对UC具有协同影响。(中华检验医学杂志,2015,38:341-345)
目的:探討TRAIL受體基因多態性與潰瘍性結腸炎( UC)的關繫。方法從2008年1月至2012年12月從溫州市4傢大型綜閤性醫院共收集380例UC患者[男215例,女165例,平均年齡(42.63±14.61)歲]和539名健康對照者[男290名,女249名,平均年齡(41.29±15.86)歲]。採用微測序技術檢測 DR4( rs20575、rs13278062), DR5( rs1047266), DcR2( rs1133782)及 OPG (rs3102735)5種單覈苷痠多態性(SNP)。採用χ2檢驗或Fisher精確概率法在UC組和對照組之間比較各SNP的等位基因及基因型頻率差異,非條件Logistic迴歸分析5種SNP與UC患者臨床病理特徵的關繫,Logistic多元迴歸分析UC的危險因素及因素間的相乘交互作用。結果與對照組比較, UC組中DR4(rs20575)的突變等位基因(G)和基因型(CG+GG)頻率均增高(3.55%比1.95%,χ2=4.512,P=0.034;6.58%比3.71%,χ2=3.938,P=0.047),而DcR2(rs1133782)的突變等位基因(A)和基因型(GA+AA)頻率均降低(6.18%比9.09%,χ2=5.183,P=0.023;11.32%比17.44%,χ2=6.589,P=0.010)。 UC組中OPG(rs3102735)的突變等位基因(T)和基因型(TT)頻率均顯著高于對照組(86.32%比81.54%,χ2=7.385,P=0.007;75.26%比66.98%,χ2=7.346,P=0.007)。 DcR2(rs1133782)野生基因型(GG)是 UC 的獨立危險因素(OR =4.937,95%CI:2.320~10.504,P <0.001);DR4(rs20575)基因型(CC)分彆與DcR2(rs1133782)基因型(GG)和OPG(rs3102735)基因型(TT)之間存在相乘交互作用(OR=0.322,95%CI:0.164~0.633,P=0.001; OR=1.580,95%CI:1.165~2.144,P=0.003)。結論 DR4(rs20575),DcR2(rs1133782)及OPG(rs3102735)基因多態性與UC相關。 DcR2(rs1133782)基因突變可能是UC的保護因素。 DR4(rs20575)分彆與DcR2(rs1133782)和OPG( rs3102735)存在交互作用,對UC具有協同影響。(中華檢驗醫學雜誌,2015,38:341-345)
목적:탐토TRAIL수체기인다태성여궤양성결장염( UC)적관계。방법종2008년1월지2012년12월종온주시4가대형종합성의원공수집380례UC환자[남215례,녀165례,평균년령(42.63±14.61)세]화539명건강대조자[남290명,녀249명,평균년령(41.29±15.86)세]。채용미측서기술검측 DR4( rs20575、rs13278062), DR5( rs1047266), DcR2( rs1133782)급 OPG (rs3102735)5충단핵감산다태성(SNP)。채용χ2검험혹Fisher정학개솔법재UC조화대조조지간비교각SNP적등위기인급기인형빈솔차이,비조건Logistic회귀분석5충SNP여UC환자림상병리특정적관계,Logistic다원회귀분석UC적위험인소급인소간적상승교호작용。결과여대조조비교, UC조중DR4(rs20575)적돌변등위기인(G)화기인형(CG+GG)빈솔균증고(3.55%비1.95%,χ2=4.512,P=0.034;6.58%비3.71%,χ2=3.938,P=0.047),이DcR2(rs1133782)적돌변등위기인(A)화기인형(GA+AA)빈솔균강저(6.18%비9.09%,χ2=5.183,P=0.023;11.32%비17.44%,χ2=6.589,P=0.010)。 UC조중OPG(rs3102735)적돌변등위기인(T)화기인형(TT)빈솔균현저고우대조조(86.32%비81.54%,χ2=7.385,P=0.007;75.26%비66.98%,χ2=7.346,P=0.007)。 DcR2(rs1133782)야생기인형(GG)시 UC 적독립위험인소(OR =4.937,95%CI:2.320~10.504,P <0.001);DR4(rs20575)기인형(CC)분별여DcR2(rs1133782)기인형(GG)화OPG(rs3102735)기인형(TT)지간존재상승교호작용(OR=0.322,95%CI:0.164~0.633,P=0.001; OR=1.580,95%CI:1.165~2.144,P=0.003)。결론 DR4(rs20575),DcR2(rs1133782)급OPG(rs3102735)기인다태성여UC상관。 DcR2(rs1133782)기인돌변가능시UC적보호인소。 DR4(rs20575)분별여DcR2(rs1133782)화OPG( rs3102735)존재교호작용,대UC구유협동영향。(중화검험의학잡지,2015,38:341-345)
Objective To investigate associations of UC with the polymorphisms of TRAIL receptors.Methods From January 2008 to December 2012, 380 consecutive UC patients [215 males and 165 females, the average age was (42.63 ±14.61) years] as well as 539 sex-and age-matched healthy individuals [290 males and 249 females, the average age was (41.29 ±15.86) years] were recruited from four large scale comprehensive hospitals in Wenzhou city.Five single nucleotide polymorphisms of DR4 (rs20575, rs13278062), DR5(rs1047266), DcR2(rs1133782) and OPG (rs3102735) were detected by a SNaPshot technique.Distributions of mutant alleles and genotypes for targeted polymorphisms in TRAIL receptors were analyzed by Chi-square test or Fisher′s exact test. By means of unconditional Logistic regression analysis, it evaluated associations between the polymorphisms and the risk of UC attack as well as the clinical features of UC patients.Furthermore, an unconditional Logistic multiple regression analysis was employed to investigate the independent risk factors of UC and their multiplicative interaction effects on UC.Results The frequencies of mutant allele (G) and genotype (CG+GG) of DR4(rs20575) were higher in UC patients than those in the controls (3.55%vs 1.95%,χ2 =4.512, P=0.034;6.58%vs 3.71%,χ2=3.938, P=0.047, respectively).However, the frequeucies of mutant allele ( A) and genotype ( GA+AA) of DcR2(rs1133782) were decreased in UC patients compared to the controls(6.18%vs 9.09%,χ2=5.183, P=0.023; 11.32% vs 17.44%, χ2 =6.589, P=0.010, respectively).The frequencies of mutant allele (T) and homozygote (TT) of OPG(rs3102735) were significantly higher in UC patients than in the controls (86.32% vs 81.54%, χ2 =7.385, P=0.007;75.26% vs 66.98%, χ2 =7.346, P=0.007, respectively) .Furthermore, the genotype (GG) of DcR2 (rs1133782) was found to be the independent risk factor for UC attack (OR=4.937, 95%CI:2.320-10.504, P<0.001).Moreover, the (GG) of DcR2(rs1133782) and (CC) of DR4(rs20575) had an interactive effect on UC (OR=0.322, 95%CI:0.164-0.633, P=0.001).The same conclusion was drawn for the ( GG) of DR4( rs20575) and (TT) of OPG(rs3102735) (OR=1.580, 95%CI:1.165-2.144, P=0.003).Conclusions The genetic polymorphisms of DR4 ( rs20575 ) , DcR2 ( rs1133782 ) and OPG ( rs3102735 ) were associated with UC. The mutation of DcR2(rs1133782) might play a protective role in UC.Moreover, the DcR2(rs1133782) and DR4(rs20575) gene had a collaborative effect on UC.So did the DR4(rs20575) and OPG(rs3102735) genes.