海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2015年
5期
59-60
,共2页
甘草配方颗粒%甘草酸%高效液相色谱法
甘草配方顆粒%甘草痠%高效液相色譜法
감초배방과립%감초산%고효액상색보법
Glycyrrhizae Granula%Glycyrrhizic acid%HPLC
目的：建立甘草配方颗粒中甘草酸的含量测定方法。方法采用 C18色谱柱，以甲醇-0.2mol· L －1醋酸铵（65∶35，醋酸调节 pH 值至4.0）为流动相，流速为1.0mL· min －1，检测波长为250nm。结果甘草酸在0.492～7.872μg之间呈现良好的线性关系，r＝0.9999；平均加样回收率为99.9％，RSD＝1.2％（n＝6）。结论本方法重复性好，能准确测定甘草配方颗粒中甘草酸的含量。
目的：建立甘草配方顆粒中甘草痠的含量測定方法。方法採用 C18色譜柱，以甲醇-0.2mol· L －1醋痠銨（65∶35，醋痠調節 pH 值至4.0）為流動相，流速為1.0mL· min －1，檢測波長為250nm。結果甘草痠在0.492～7.872μg之間呈現良好的線性關繫，r＝0.9999；平均加樣迴收率為99.9％，RSD＝1.2％（n＝6）。結論本方法重複性好，能準確測定甘草配方顆粒中甘草痠的含量。
목적：건립감초배방과립중감초산적함량측정방법。방법채용 C18색보주，이갑순-0.2mol· L －1작산안（65∶35，작산조절 pH 치지4.0）위류동상，류속위1.0mL· min －1，검측파장위250nm。결과감초산재0.492～7.872μg지간정현량호적선성관계，r＝0.9999；평균가양회수솔위99.9％，RSD＝1.2％（n＝6）。결론본방법중복성호，능준학측정감초배방과립중감초산적함량。
OBJECTIVE To establish the method for determining of glycyrrhizic acid in Glycyrrhizae Granula by HPLC.METHODS C18 column was used with the mobile phase consisted of methanol-0.2mol· L-1 ammonium acetate (65∶35,adjusted pH to 4.0 with CH3COOH),the flow rate was 1.0mL· min -1,the detection wavelength was 250nm.RESULTS the calibration curve was linear at a range of 0.492~7.872μg for glycyrrhizic acid ( r=0.9999),the average recovery was 99.9%and RSD was 1.2%(n=6).CONCLUSION the method was accurate with a good reproducibility and can be used as a quantitative analysis of Glycyrrhizic acid in Glycyrrhizae Granula.
