广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2015年
10期
118-120,133
,共4页
合成雌激素%毛细管电泳%β-环糊精
閤成雌激素%毛細管電泳%β-環糊精
합성자격소%모세관전영%β-배호정
synthetic estrogens%micellar capillary electrophoresis%β-cyclodextrin
建立了以β-环糊精为添加剂的胶束电动毛细管电泳-电化学检测分析结构、性质相似的合成雌激素新方法。系统考察分离缓冲体系以及检测条件。在优化条件下,缓冲溶液为H3PO4(25 mmol/L)-SDS(30 mmol/L)-β-CD(7 mmol/L),应用负极电动进样正极检测的方式,对己烯雌酚,己烷雌酚和双烯雌酚的检出限分别为6.5×10-6 mol/L,4.8×10-6 mol/L和7.9×10-6 mol/L。并成功用于药物中己烯雌酚含量的测定,结果令人满意。
建立瞭以β-環糊精為添加劑的膠束電動毛細管電泳-電化學檢測分析結構、性質相似的閤成雌激素新方法。繫統攷察分離緩遲體繫以及檢測條件。在優化條件下,緩遲溶液為H3PO4(25 mmol/L)-SDS(30 mmol/L)-β-CD(7 mmol/L),應用負極電動進樣正極檢測的方式,對己烯雌酚,己烷雌酚和雙烯雌酚的檢齣限分彆為6.5×10-6 mol/L,4.8×10-6 mol/L和7.9×10-6 mol/L。併成功用于藥物中己烯雌酚含量的測定,結果令人滿意。
건립료이β-배호정위첨가제적효속전동모세관전영-전화학검측분석결구、성질상사적합성자격소신방법。계통고찰분리완충체계이급검측조건。재우화조건하,완충용액위H3PO4(25 mmol/L)-SDS(30 mmol/L)-β-CD(7 mmol/L),응용부겁전동진양정겁검측적방식,대기희자분,기완자분화쌍희자분적검출한분별위6.5×10-6 mol/L,4.8×10-6 mol/L화7.9×10-6 mol/L。병성공용우약물중기희자분함량적측정,결과령인만의。
A method based on micellar capillary electrophoresis with β-cyclodextrin has been developed for the determination of synthetic estrogens ( including diethylstilbestrol, dienoestrol and hexestrol ) . The effects of several important factors that influence on separation and detection were investigated. Under the optimum conditions, the estrogens could be separated in 25 mmol/L H3 PO4 and 30 mmol/L SDS containing 7 mmol/Lβ-CD at an applied voltage of -20 kV. The limits of detection for diethylstilbestrol, dienoestrol and hexestrol were 6. 5 × 10-6 , 4. 8 × 10-6 and 7. 9 × 10-6 ( S/N=3 ) , respectively. The method also had been applied to the analysis of practical samples spiked with synthetic estrogens, and results are satisfactory.
