现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
12期
1641-1643
,共3页
PD-L1%CIK%肺腺癌%RNAi
PD-L1%CIK%肺腺癌%RNAi
PD-L1%CIK%폐선암%RNAi
PD-L1%CIK%lung adenocarcinoma%RNAi
目的:观察沉默肺癌A549细胞的程序性死亡受体1(programmed death ligand 1,PD-L1)基因表达后,CIK细胞对其体外抑癌的作用.方法:分离外周血单个核细胞(peripheral blood mononuclear cell,PBMC),加入细胞因子(cytokine,CK),体外细胞因子诱导为杀伤细胞(cytokine induced killer cells,CIK cells).将已构建的PD-L1 shRNA重组质粒pGPU6/PD-L1,应用脂质体转染法转染入A549细胞.RT-PCR法检测PD-L1基因的表达;Western blotting法检测PD-L1蛋白的表达;CCK8法检测CIK细胞对各组A549细胞的杀伤率.结果:成功将pGPU6/PD-L1转染入A549细胞;RT-PCR结果显示pGPU6/PD-L1使A549细胞PD-L1基因表达水平降低;Western blotting结果显示pGPU6/PD-L1转染A549细胞使PD-L1蛋白表达减少;CCK8结果显示pGPU6/PD-L1能够增强CIK细胞对A549细胞的体外抑瘤能力.结论:下调肺癌细胞A549的PD-L1 mRNA表达,MTT证明能够增强CIK细胞对其体外杀伤作用.
目的:觀察沉默肺癌A549細胞的程序性死亡受體1(programmed death ligand 1,PD-L1)基因錶達後,CIK細胞對其體外抑癌的作用.方法:分離外週血單箇覈細胞(peripheral blood mononuclear cell,PBMC),加入細胞因子(cytokine,CK),體外細胞因子誘導為殺傷細胞(cytokine induced killer cells,CIK cells).將已構建的PD-L1 shRNA重組質粒pGPU6/PD-L1,應用脂質體轉染法轉染入A549細胞.RT-PCR法檢測PD-L1基因的錶達;Western blotting法檢測PD-L1蛋白的錶達;CCK8法檢測CIK細胞對各組A549細胞的殺傷率.結果:成功將pGPU6/PD-L1轉染入A549細胞;RT-PCR結果顯示pGPU6/PD-L1使A549細胞PD-L1基因錶達水平降低;Western blotting結果顯示pGPU6/PD-L1轉染A549細胞使PD-L1蛋白錶達減少;CCK8結果顯示pGPU6/PD-L1能夠增彊CIK細胞對A549細胞的體外抑瘤能力.結論:下調肺癌細胞A549的PD-L1 mRNA錶達,MTT證明能夠增彊CIK細胞對其體外殺傷作用.
목적:관찰침묵폐암A549세포적정서성사망수체1(programmed death ligand 1,PD-L1)기인표체후,CIK세포대기체외억암적작용.방법:분리외주혈단개핵세포(peripheral blood mononuclear cell,PBMC),가입세포인자(cytokine,CK),체외세포인자유도위살상세포(cytokine induced killer cells,CIK cells).장이구건적PD-L1 shRNA중조질립pGPU6/PD-L1,응용지질체전염법전염입A549세포.RT-PCR법검측PD-L1기인적표체;Western blotting법검측PD-L1단백적표체;CCK8법검측CIK세포대각조A549세포적살상솔.결과:성공장pGPU6/PD-L1전염입A549세포;RT-PCR결과현시pGPU6/PD-L1사A549세포PD-L1기인표체수평강저;Western blotting결과현시pGPU6/PD-L1전염A549세포사PD-L1단백표체감소;CCK8결과현시pGPU6/PD-L1능구증강CIK세포대A549세포적체외억류능력.결론:하조폐암세포A549적PD-L1 mRNA표체,MTT증명능구증강CIK세포대기체외살상작용.
