实用口腔医学杂志
實用口腔醫學雜誌
실용구강의학잡지
JOURNAL OF PRACTICAL STOMATOLOGY
2015年
3期
309-312
,共4页
浓缩生长因子(CGF)%骨结合%成骨细胞%种植体
濃縮生長因子(CGF)%骨結閤%成骨細胞%種植體
농축생장인자(CGF)%골결합%성골세포%충식체
Concentrate growth factors(CGF)%Osseointegration%Osteoblast%Implant
目的:探讨浓缩生长因子提取液(CGFe)对 MC3T3-E1细胞增殖、分化的影响。方法:实验组和对照组分别使用含有及不含有 CGFe 的α-MEM培养液培养 MC3T3-E1细胞。培养1、3、5 d 后 MTT 法测定细胞增殖,ALP 试剂盒检测碱性磷酸酶活性;培养7、14 d 后茜素红染色观察钙结节形成;荧光实时定量 PCR 测定 RUNX2和 OSX mRNA 的表达。结果:随着培养时间延长,实验组的细胞增殖、碱性磷酸酶活性、钙结节数目及基因表达量均高于对照组(P <0.05)。结论:浓缩生长因子提取液能有效地促进 MC3T3-E1细胞的增殖、分化。
目的:探討濃縮生長因子提取液(CGFe)對 MC3T3-E1細胞增殖、分化的影響。方法:實驗組和對照組分彆使用含有及不含有 CGFe 的α-MEM培養液培養 MC3T3-E1細胞。培養1、3、5 d 後 MTT 法測定細胞增殖,ALP 試劑盒檢測堿性燐痠酶活性;培養7、14 d 後茜素紅染色觀察鈣結節形成;熒光實時定量 PCR 測定 RUNX2和 OSX mRNA 的錶達。結果:隨著培養時間延長,實驗組的細胞增殖、堿性燐痠酶活性、鈣結節數目及基因錶達量均高于對照組(P <0.05)。結論:濃縮生長因子提取液能有效地促進 MC3T3-E1細胞的增殖、分化。
목적:탐토농축생장인자제취액(CGFe)대 MC3T3-E1세포증식、분화적영향。방법:실험조화대조조분별사용함유급불함유 CGFe 적α-MEM배양액배양 MC3T3-E1세포。배양1、3、5 d 후 MTT 법측정세포증식,ALP 시제합검측감성린산매활성;배양7、14 d 후천소홍염색관찰개결절형성;형광실시정량 PCR 측정 RUNX2화 OSX mRNA 적표체。결과:수착배양시간연장,실험조적세포증식、감성린산매활성、개결절수목급기인표체량균고우대조조(P <0.05)。결론:농축생장인자제취액능유효지촉진 MC3T3-E1세포적증식、분화。
Objective:To evaluate the effect of concentrate growth factor extract(CGFe)on the proliferation and differentiation of MC3T3-E1 osteoblasts.Methods:MC3T3-1 cells were cultured with and without CGFe respectively.At 1 ,3,5 d of culture the pro-liferation of the cells was detected by MTT assay,the activity of alkaline phosphatase (ALP)of the cells was examined by ALP kit;at 1 4,21 d of culture the calcium nodus formation was observed by Alizarin red staining;RUNX2 and OSX expression was quantified by real-time PCR.Results:With the extension of culture time,the proliferation,ALP activity,the calcium nodus and the level of RUNX2 and OSX mRNA of the experimental group were increased more than those of the control group.Conclusion:CGFe may pro-mote the proliferation and differentiation of osteoblasts.