中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
22期
1762-1764
,共3页
蒋丽潇%李东明%尚盼盼%孙婷婷%肖秀美
蔣麗瀟%李東明%尚盼盼%孫婷婷%肖秀美
장려소%리동명%상반반%손정정%초수미
皮肤疾病,细菌性%微生物敏感性试验%抗药性,细菌
皮膚疾病,細菌性%微生物敏感性試驗%抗藥性,細菌
피부질병,세균성%미생물민감성시험%항약성,세균
Skin diseases,bacterial%Microbial sensitivity tests%Drug resistance,bacterial
目的 了解感染相关皮肤病细菌多样性及其耐药性.方法 回顾性分析2010年3月至2011年5月北京大学第三医院皮肤科临床拟诊为皮肤细菌感染或继发细菌感染且标本细菌培养阳性的54例患者资料.标本来自皮损痂皮或组织、血液、脓液、渗出液等.应用VITEKⅡ全自动细菌鉴定仪进行细菌鉴定,K-B法进行药敏试验,结果按CLSI-M100.S21标准判读.结果 54例患者共鉴定出63株细菌,分布于12个属,共22种.涉及的菌种有表皮葡萄球菌、金黄色葡萄球菌、头状葡萄球菌、人葡萄球菌、滕黄微球菌、粪肠球菌D群、甲型溶血链球菌、化脓链球菌A群、无乳链球菌、棒状杆菌属、枯草芽孢杆菌、蜡样芽胞杆菌、鲍曼不动杆菌、洛菲不动杆菌、铜绿假单胞菌、阴沟肠杆菌、放射根瘤菌、少动鞘氨醇单胞菌、干燥奈瑟菌、奈瑟菌属某些菌等.甲氧西林耐药凝固酶阴性葡萄球菌占所分离凝固酶阴性葡萄球菌的46.4%(13/28).葡萄球菌属对氨苄西林、青霉素、阿奇霉素、头孢西丁、克林霉素、复方磺胺甲(噁)唑的耐药率分别为88.6% (31/35)、88.6%(31/35)、68.6% (24/35)、37.1(13/35)、28.6(10/35)、26.5(9/34).革兰阴性杆菌对阿莫西林、丁胺卡那霉素、头孢他啶等药物较敏感.结论 皮肤细菌感染病原菌种类繁多,耐药菌株的出现或为难治性感染的重要原因.
目的 瞭解感染相關皮膚病細菌多樣性及其耐藥性.方法 迴顧性分析2010年3月至2011年5月北京大學第三醫院皮膚科臨床擬診為皮膚細菌感染或繼髮細菌感染且標本細菌培養暘性的54例患者資料.標本來自皮損痂皮或組織、血液、膿液、滲齣液等.應用VITEKⅡ全自動細菌鑒定儀進行細菌鑒定,K-B法進行藥敏試驗,結果按CLSI-M100.S21標準判讀.結果 54例患者共鑒定齣63株細菌,分佈于12箇屬,共22種.涉及的菌種有錶皮葡萄毬菌、金黃色葡萄毬菌、頭狀葡萄毬菌、人葡萄毬菌、滕黃微毬菌、糞腸毬菌D群、甲型溶血鏈毬菌、化膿鏈毬菌A群、無乳鏈毬菌、棒狀桿菌屬、枯草芽孢桿菌、蠟樣芽胞桿菌、鮑曼不動桿菌、洛菲不動桿菌、銅綠假單胞菌、陰溝腸桿菌、放射根瘤菌、少動鞘氨醇單胞菌、榦燥奈瑟菌、奈瑟菌屬某些菌等.甲氧西林耐藥凝固酶陰性葡萄毬菌佔所分離凝固酶陰性葡萄毬菌的46.4%(13/28).葡萄毬菌屬對氨芐西林、青黴素、阿奇黴素、頭孢西丁、剋林黴素、複方磺胺甲(噁)唑的耐藥率分彆為88.6% (31/35)、88.6%(31/35)、68.6% (24/35)、37.1(13/35)、28.6(10/35)、26.5(9/34).革蘭陰性桿菌對阿莫西林、丁胺卡那黴素、頭孢他啶等藥物較敏感.結論 皮膚細菌感染病原菌種類繁多,耐藥菌株的齣現或為難治性感染的重要原因.
목적 료해감염상관피부병세균다양성급기내약성.방법 회고성분석2010년3월지2011년5월북경대학제삼의원피부과림상의진위피부세균감염혹계발세균감염차표본세균배양양성적54례환자자료.표본래자피손가피혹조직、혈액、농액、삼출액등.응용VITEKⅡ전자동세균감정의진행세균감정,K-B법진행약민시험,결과안CLSI-M100.S21표준판독.결과 54례환자공감정출63주세균,분포우12개속,공22충.섭급적균충유표피포도구균、금황색포도구균、두상포도구균、인포도구균、등황미구균、분장구균D군、갑형용혈련구균、화농련구균A군、무유련구균、봉상간균속、고초아포간균、사양아포간균、포만불동간균、락비불동간균、동록가단포균、음구장간균、방사근류균、소동초안순단포균、간조내슬균、내슬균속모사균등.갑양서림내약응고매음성포도구균점소분리응고매음성포도구균적46.4%(13/28).포도구균속대안변서림、청매소、아기매소、두포서정、극림매소、복방광알갑(오)서적내약솔분별위88.6% (31/35)、88.6%(31/35)、68.6% (24/35)、37.1(13/35)、28.6(10/35)、26.5(9/34).혁란음성간균대아막서림、정알잡나매소、두포타정등약물교민감.결론 피부세균감염병원균충류번다,내약균주적출현혹위난치성감염적중요원인.
Objective To explore the bacterial diversity and resistance in infection-related skin disorders.Methods The samples of blood,pyogenic fluid,exudate and skin dander were collected from 54 outpatients of chronic and recurrent skin disease and cultured for positive pathogens in the dermatological department of Peking University Third hospital from March 2010 to May 2011.Also their drug susceptibilities were examined.Results Among 63 bacterial strains of 22 species in 12 genus,the pathogens were Staphylococcus epidermidis,Staphylococcus aureus,Micrococcus luteus,group A Streptococcus pyogenes,Staphylococcus agalactiae,Corynebacterium sp.,Bacillus subtilis,Bacillus cereus,Acinetobacter baumanii,A.lwoffii,Pseudomonas aeruginosa,Enterobacter cloacae,Rhizobium radiobacter,Sphingomonas paucimobilis,Enterococcus faecalis,Neisseria sicca and Neisseria gonorrhoeae.The percentage of methicillin-resistant coagulase negative staphylococci (MRCNS) was 46.4% (13/28) while the resistant rates of Styphylococci to ampicillin,penicillin,azithromycin,cefoxitin,clindamycin and SMZ-TMP were 88.6% (31/35),88.6% (31/35),68.6% (24/35),37.1 (13/35),28.6 (10/35) and 26.5 (9/34) respectively.Gram negative bacilli were sensitive to ampicillin,amikacin sulfate,ceftazidime.Conclusion There are a wide range of pathogenic bacterial species among refractory infection of outpatients.And drug resistance is among the reasons for refractory infections.