CAJ | 학술논문

弓形虫感染可激发宿主产生Th1型免疫反应，因此研究弓形虫疫苗尤其需要关注系统性T细胞免疫应答与保护力的关系。本研究被弓形虫Pru株包囊口服感染BALB／c小鼠，成功建立了弓形虫慢性感染小鼠模型。慢性感染小鼠的脾细胞用弓形虫可溶性抗原刺激后可以产生特异性增殖。进一步分析发现弓形虫特异性CD3＋T、 CD4＋T和CD8＋T细胞都可以产生IFN-γ。本研究建立的弓形虫慢性感染小鼠模型可以产生较强的系统性T细胞免疫应答，为弓形虫疫苗研制的效果评价提供重要参考。
궁형충감염가격발숙주산생Th1형면역반응，인차연구궁형충역묘우기수요관주계통성T세포면역응답여보호력적관계。본연구피궁형충Pru주포낭구복감염BALB／c소서，성공건립료궁형충만성감염소서모형。만성감염소서적비세포용궁형충가용성항원자격후가이산생특이성증식。진일보분석발현궁형충특이성CD3＋T、 CD4＋T화CD8＋T세포도가이산생IFN-γ。본연구건립적궁형충만성감염소서모형가이산생교강적계통성T세포면역응답，위궁형충역묘연제적효과평개제공중요삼고。
Toxoplasma gondii infection elicits a Th1-biased inflammatory response.As the protection induced by T.gondii vaccine is highly correlated with the systemic T-cell immune response, here we detected the cellular immune responses in chronically infected BALB/c mice.Splenocytes collected from chronically infected mice exhibited stronger lymphocyte proliferation after the stimulation of cells with soluble tachyzoite antigens.What is more, IFN-γsecretion was detected in CD3 +T, CD4 +T and CD8 +T cells after the stimulation of splenocytes with antigens.In summary, T.gondii chronic infected mice showed stronger systemic T-cell immune response, which would provide valuable data for the evaluation of the efficiency of T.gondii vaccine.