山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
20期
22-24
,共3页
陈旖鹛%张彤%李万根%陈勇霞%牛建民%王芳%李子昊
陳旖鹛%張彤%李萬根%陳勇霞%牛建民%王芳%李子昊
진의미%장동%리만근%진용하%우건민%왕방%리자호
妊娠并发症%线粒体DNA含量%妊娠糖尿病%妊娠早期
妊娠併髮癥%線粒體DNA含量%妊娠糖尿病%妊娠早期
임신병발증%선립체DNA함량%임신당뇨병%임신조기
pregnancy complications%mitochondrial DNA content%gestational diabetes mellitus%first trimester of pregnancy
目的：观察妊娠早期外周血白细胞线粒体DNA（ mtDNA）含量是否与妊娠糖尿病（ GDM）患病相关。方法选取妊娠＜12孕周者206例，应用实时定量PCR法检测研究对象外周血妊娠早期白细胞mtDNA含量的值，以mtDNA与核DNA（nDNA）含量比值（mtDNA／nDNA）表示；根据mtDNA含量中位数分为低mtDNA含量组（n＝103）和高mtDNA含量组（ n＝103）。测定妊娠早期空腹葡萄糖、空腹胰岛素等葡萄糖代谢指标，计算胰岛素抵抗指数（ HOMA-IR）；妊娠24～28周行口服葡萄糖耐量试验，分别统计两组GDM患病率。结果低mtDNA含量组和高mtDNA含量组妊娠24～28周GDM患病率分别为10．7％和6．8％，P＞0．05；两组妊娠早期空腹葡萄糖、胰岛素和HOMA-IR比较，P均＞0．05。结论妊娠早期外周血白细胞mtDNA含量与GDM无明显相关性。
目的：觀察妊娠早期外週血白細胞線粒體DNA（ mtDNA）含量是否與妊娠糖尿病（ GDM）患病相關。方法選取妊娠＜12孕週者206例，應用實時定量PCR法檢測研究對象外週血妊娠早期白細胞mtDNA含量的值，以mtDNA與覈DNA（nDNA）含量比值（mtDNA／nDNA）錶示；根據mtDNA含量中位數分為低mtDNA含量組（n＝103）和高mtDNA含量組（ n＝103）。測定妊娠早期空腹葡萄糖、空腹胰島素等葡萄糖代謝指標，計算胰島素牴抗指數（ HOMA-IR）；妊娠24～28週行口服葡萄糖耐量試驗，分彆統計兩組GDM患病率。結果低mtDNA含量組和高mtDNA含量組妊娠24～28週GDM患病率分彆為10．7％和6．8％，P＞0．05；兩組妊娠早期空腹葡萄糖、胰島素和HOMA-IR比較，P均＞0．05。結論妊娠早期外週血白細胞mtDNA含量與GDM無明顯相關性。
목적：관찰임신조기외주혈백세포선립체DNA（ mtDNA）함량시부여임신당뇨병（ GDM）환병상관。방법선취임신＜12잉주자206례，응용실시정량PCR법검측연구대상외주혈임신조기백세포mtDNA함량적치，이mtDNA여핵DNA（nDNA）함량비치（mtDNA／nDNA）표시；근거mtDNA함량중위수분위저mtDNA함량조（n＝103）화고mtDNA함량조（ n＝103）。측정임신조기공복포도당、공복이도소등포도당대사지표，계산이도소저항지수（ HOMA-IR）；임신24～28주행구복포도당내량시험，분별통계량조GDM환병솔。결과저mtDNA함량조화고mtDNA함량조임신24～28주GDM환병솔분별위10．7％화6．8％，P＞0．05；량조임신조기공복포도당、이도소화HOMA-IR비교，P균＞0．05。결론임신조기외주혈백세포mtDNA함량여GDM무명현상관성。
Objective To investigate whether white blood cell mitochondrial DNA ( mtDNA) content in the first tri-mester of pregnancy is associated with gestational diabetes mellitus ( GDM) .Methods Data and blood samples were col-lected from 206 females who were diagnosed as pregnancy within 12 weeks.The mtDNA quantification using nuclear DNA ( nDNA) as a reference was analyzed by real-time quantitative polymerase chain reaction ( PCR) .According to the median value of the mtDNA/nDNA ratio, the subjects were divided into two groups: lower mtDNA content group (n=103) and higher mtDNA content group (n=103).The fasting glucose and fasting insulin were measured and homeostasis model as-sessment of IR (HOMA-IR) was calculated.Oral glucose tolerance test (OGTT) was taken between week 24 and 28 of gestation, and the prevalence of GDM in the two groups was observed .Results In the first trimester of pregnancy from week 24 to week 28, the prevalence of GDM in the lower mtDNA content group and higher mtDNA content group was 10. 7%and 6.8%, respectively (P>0.05).There was no statistical significance in fasting glucose , fasting insulin and HO-MA-IR between thess two groups (all P>0.05).Conclusion The content of white blood cell mtDNA in the first trimester of pregnancy is not a risk factor for GDM .
