CAJ | 학술논문

目的：建立高效液相色谱法测定大花罗布麻叶中异槲皮苷含量的方法。方法采用高效液相色谱法对样品中异槲皮苷进行定量分析，以十八烷基硅烷键合硅胶为填充剂；甲醇-乙腈-0．3％磷酸（11∶10∶79）为流动相，柱温为35℃，检测波长为354 nm。结果异槲皮苷进样量在浓度为0．0202～0．2020 mg／mL （r ＝0．9999）范围内与其峰面积呈良好线性关系。平均回收率为104．45％（n ＝6），RSD 为0．45％。10批不同产地样品中异槲皮苷的含量为0．320％～0．392％，平均含量为0．37％。结论 HPLC 色谱法对大花罗布麻叶中异槲皮苷进行测定，方法简便，精确，重现性好，为有效制订质量标准提供科学依据。
목적：건립고효액상색보법측정대화라포마협중이곡피감함량적방법。방법채용고효액상색보법대양품중이곡피감진행정량분석，이십팔완기규완건합규효위전충제；갑순-을정-0．3％린산（11∶10∶79）위류동상，주온위35℃，검측파장위354 nm。결과이곡피감진양량재농도위0．0202～0．2020 mg／mL （r ＝0．9999）범위내여기봉면적정량호선성관계。평균회수솔위104．45％（n ＝6），RSD 위0．45％。10비불동산지양품중이곡피감적함량위0．320％～0．392％，평균함량위0．37％。결론 HPLC 색보법대대화라포마협중이곡피감진행측정，방법간편，정학，중현성호，위유효제정질량표준제공과학의거。
Objective To establish a method for the determination of isoquercitrin in leaves of Pocvnum hendersonii (Hook.f.)Woodson by HPLC.Methods HPLC method was adopted for a quantitative analysis of isoquercitrin.It was carried out on C18 column,with the mobile phase of methnal-acetonitrile-0.3% phosphoric acid water (11∶ 10∶ 79),the flow rate of 1.00 ml/min,the column temperature of 35℃ and the wavelength for detection of 354 nm.Results There were good linear relationships between the peak area and concentration at the range of 0.020 2~0.202 mg/mL for isoquercitrin (r =0.999 9).The average recovery rate of isoquercitrin was 104.45% (n =6),with RSD of 0.45%.The average content of ten different samples was 0.37%.Conclusion HPLC method to determine the isoquercitrin in leaves of Pocvnum hendersonii (Hook.f.)Woodson was proved to be simple,accurate and reproducible,which can provide scientific basis for quality control of leaves of Pocvnum hendersonii (Hook.f.)Woodson.