山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
20期
7-9
,共3页
王贲士%乔录新%张玉林%封晓昆%陈德喜%郭洪亮
王賁士%喬錄新%張玉林%封曉昆%陳德喜%郭洪亮
왕분사%교록신%장옥림%봉효곤%진덕희%곽홍량
结肠癌%肝癌%线粒体%p53%tRNAleu区序列%突变
結腸癌%肝癌%線粒體%p53%tRNAleu區序列%突變
결장암%간암%선립체%p53%tRNAleu구서렬%돌변
colonic carcinoma%liver carcinoma%mitochondria%p53%sequence of tRNAleu region%mutation
目的：研究肿瘤患者线粒体DNA（ mtDNA）中tRNAleu区序列3264位点上T→C突变与肿瘤发生及p53突变的关系。方法提取接受手术治疗的28例肝癌及35例结肠癌患者的癌组织与癌旁组织总DNA，采用PCR法扩增mtDNA中的tRNAleu区以及p53基因全长并测序。结果所有癌组织中未见3264位点T→C的突变，但有16例在其他位点发现了突变；所有癌旁组织中未见3264位点T→C的突变，有3例在其他位点发生突变。在癌组织中发现17例p53基因突变；结肠癌组织p53突变9例，其中存在tRNAleu区突变6例、无tRNAleu区突变3例，P＜0．05；肝癌组织p53突变8例，其中存在tRNAlen区突变5例，无tRNAlen区突变3例，P＜0．05。结论 tRNAleu区3264位点上T→C的突变暂不能认为与肿瘤的发生存在直接关系，该区域其他位点的突变可能是由于p53突变后丧失对该基因的保护而继发产生的。
目的：研究腫瘤患者線粒體DNA（ mtDNA）中tRNAleu區序列3264位點上T→C突變與腫瘤髮生及p53突變的關繫。方法提取接受手術治療的28例肝癌及35例結腸癌患者的癌組織與癌徬組織總DNA，採用PCR法擴增mtDNA中的tRNAleu區以及p53基因全長併測序。結果所有癌組織中未見3264位點T→C的突變，但有16例在其他位點髮現瞭突變；所有癌徬組織中未見3264位點T→C的突變，有3例在其他位點髮生突變。在癌組織中髮現17例p53基因突變；結腸癌組織p53突變9例，其中存在tRNAleu區突變6例、無tRNAleu區突變3例，P＜0．05；肝癌組織p53突變8例，其中存在tRNAlen區突變5例，無tRNAlen區突變3例，P＜0．05。結論 tRNAleu區3264位點上T→C的突變暫不能認為與腫瘤的髮生存在直接關繫，該區域其他位點的突變可能是由于p53突變後喪失對該基因的保護而繼髮產生的。
목적：연구종류환자선립체DNA（ mtDNA）중tRNAleu구서렬3264위점상T→C돌변여종류발생급p53돌변적관계。방법제취접수수술치료적28례간암급35례결장암환자적암조직여암방조직총DNA，채용PCR법확증mtDNA중적tRNAleu구이급p53기인전장병측서。결과소유암조직중미견3264위점T→C적돌변，단유16례재기타위점발현료돌변；소유암방조직중미견3264위점T→C적돌변，유3례재기타위점발생돌변。재암조직중발현17례p53기인돌변；결장암조직p53돌변9례，기중존재tRNAleu구돌변6례、무tRNAleu구돌변3례，P＜0．05；간암조직p53돌변8례，기중존재tRNAlen구돌변5례，무tRNAlen구돌변3례，P＜0．05。결론 tRNAleu구3264위점상T→C적돌변잠불능인위여종류적발생존재직접관계，해구역기타위점적돌변가능시유우p53돌변후상실대해기인적보호이계발산생적。
Objective To investigate the relationships of T→C mutation of 3264 site in tRNAleu region of mitochon-drial DNA (mtDNA) with tumorigenesis and p53 mutation.Methods The whole DNA was extracted from carcinoma and adjacent tissues of 28 patients with liver carcinoma and 35 patients with colon cancer .Then tRNAleu region of mtDNA and p53 gene were amplified by PCR and sequenced .Results No T→C mutation was found in 3264 site, but 16 cases of mu-tations in the other sites were discovered in the carcinoma tissues .No T→C mutation was found in 3264 site, but 3 cases of mutations were discovered in the adjacent tissues .Seventeen cases of p 53 gene mutation were found in the carcinoma tis-sues;9 cases of p53 mutation were found in patients with colon carcinoma , including 6 cases of tRNAleu mutation and 3 cases of non-tRNAleu mutation (P<0.05);and 8 cases of p53 mutation were found in patients with liver cancer , inclu-ding 5 cases of tRNAleu mutation and 3 cases of non-tRNAleu mutation (P<0.05).Conclusions The T→C mutation in 3264 site of tRNAleu region can′t be considered to have relationship with tumorigenesis .The mutations in other sites of tR-NAleu region are due to p53 mutation, so the gene loses its protection , and thus the mutation occurs .
