CAJ | 학술논문

目的检测原发性高血压患者单个核细胞线粒体呼吸功能，探讨外周血单个核细胞线粒体呼吸功能与血压之间的关系。方法分别纳入原发性高血压患者和对照组的健康志愿者各20例，应用 Oxygraph-2K 线粒体呼吸功能测定仪对参与者外周血单个核细胞线粒体呼吸功能进行测定，并对线粒体呼吸功能指标与血压进行相关性分析。结果原发性高血压组单个核细胞的呼吸控制比（RCR）、基态呼吸、线粒体复合物（C）Ⅰ的氧化磷酸化的能力（CⅠOXPHOS ）、CⅡOXPHOS、CⅠ＋ⅡOXPHOS、CⅠ＋Ⅱ非耦联状态下的电子传递能力（CⅠ＋ⅡETS ）明显低于对照组（P ＜0．05），其中 RCR 与 CⅠOXPHOS同收缩压及舒张压呈负相关。结论原发性高血压患者外周血单个核细胞线粒体存在呼吸功能障碍，同时线粒体呼吸功能与血压变化相关，提示外周血单个核细胞的线粒体呼吸功能在原发性高血压发病中有一定作用。
목적검측원발성고혈압환자단개핵세포선립체호흡공능，탐토외주혈단개핵세포선립체호흡공능여혈압지간적관계。방법분별납입원발성고혈압환자화대조조적건강지원자각20례，응용 Oxygraph-2K 선립체호흡공능측정의대삼여자외주혈단개핵세포선립체호흡공능진행측정，병대선립체호흡공능지표여혈압진행상관성분석。결과원발성고혈압조단개핵세포적호흡공제비（RCR）、기태호흡、선립체복합물（C）Ⅰ적양화린산화적능력（CⅠOXPHOS ）、CⅡOXPHOS、CⅠ＋ⅡOXPHOS、CⅠ＋Ⅱ비우련상태하적전자전체능력（CⅠ＋ⅡETS ）명현저우대조조（P ＜0．05），기중 RCR 여 CⅠOXPHOS동수축압급서장압정부상관。결론원발성고혈압환자외주혈단개핵세포선립체존재호흡공능장애，동시선립체호흡공능여혈압변화상관，제시외주혈단개핵세포적선립체호흡공능재원발성고혈압발병중유일정작용。
Objective To detect the mitochondrial respiratory functions of human peripheral blood mononuclear cells in essential hypertensive patients,explore the relationship between mitochondrial respiratory function and blood pressure.Methods Totally 40 cases of participants were enrolled in our study,including 20 cases of essential hypertensive patients and 20 cases of healthy controls.Mitochondrial respiratory functions of subjects were detected by Oxygraph-2K,and the relationship between blood pres-sure and mitochondrial respiratory function was analyzed.Results The values of respiratory control ratio (RCR),routine respiratory rate,CⅠOXPHOS ,CⅡOXPHOS ,CⅠ +ⅡOXPHOS and CⅡETS in hypertension group were significantly reduced compared with those in normal controls.Meanwhile,the values of RCR and CⅠOXPHOS were negatively correlated with SBP and DBP.Conclusion Mitochondria respiratory dysfunc-tion of peripheral blood mononuclear cells occurred in essential hypertensive patients,which was correlated with blood pressure changes,indicating that the mitochondrial respiratory functions of peripheral blood mononuclear cells may play a role in the pathogenesis of essential hypertension.